Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_413 and Pf6N2E2_414 are separated by 2 nucleotides Pf6N2E2_414 and Pf6N2E2_415 are separated by 17 nucleotides Pf6N2E2_415 and Pf6N2E2_416 overlap by 4 nucleotides
Pf6N2E2_413 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 481,369 to 482,442
_413
Pf6N2E2_414 - Chemotaxis protein CheD, at 482,445 to 482,921
_414
Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775
_415
Pf6N2E2_416 - Positive regulator of CheA protein activity (CheW), at 483,772 to 484,308
_416
Position (kb) 482
483 Strain fitness (log2 ratio) -2
-1
0
1
2 at 481.664 kb on + strand, within Pf6N2E2_413 at 481.664 kb on + strand, within Pf6N2E2_413 at 482.422 kb on + strand at 482.422 kb on + strand at 482.422 kb on + strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.423 kb on - strand at 482.442 kb on + strand at 482.461 kb on - strand at 482.461 kb on - strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.462 kb on + strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.463 kb on - strand at 482.481 kb on + strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.482 kb on - strand at 482.740 kb on + strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.741 kb on - strand, within Pf6N2E2_414 at 482.743 kb on - strand, within Pf6N2E2_414 at 482.743 kb on - strand, within Pf6N2E2_414 at 482.909 kb on - strand at 482.909 kb on - strand at 482.909 kb on - strand at 483.025 kb on - strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.090 kb on + strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.091 kb on - strand, within Pf6N2E2_415 at 483.240 kb on + strand, within Pf6N2E2_415 at 483.241 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.315 kb on - strand, within Pf6N2E2_415 at 483.393 kb on + strand, within Pf6N2E2_415 at 483.394 kb on - strand, within Pf6N2E2_415 at 483.465 kb on - strand, within Pf6N2E2_415 at 483.465 kb on - strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.548 kb on + strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.549 kb on - strand, within Pf6N2E2_415 at 483.588 kb on + strand, within Pf6N2E2_415 at 483.597 kb on + strand, within Pf6N2E2_415 at 483.597 kb on + strand, within Pf6N2E2_415 at 483.598 kb on - strand, within Pf6N2E2_415 at 483.612 kb on + strand, within Pf6N2E2_415 at 483.613 kb on - strand, within Pf6N2E2_415 at 483.691 kb on - strand, within Pf6N2E2_415 at 483.691 kb on - strand, within Pf6N2E2_415 at 483.859 kb on + strand, within Pf6N2E2_416 at 483.859 kb on + strand, within Pf6N2E2_416
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 481,664 + Pf6N2E2_413 0.27 -0.5 481,664 + Pf6N2E2_413 0.27 +0.9 482,422 + +1.9 482,422 + +0.9 482,422 + -0.1 482,423 - +0.9 482,423 - -1.7 482,423 - +0.9 482,423 - -0.9 482,423 - -0.1 482,423 - -2.1 482,423 - -0.2 482,423 - -0.7 482,442 + -0.0 482,461 - +0.9 482,461 - -0.5 482,462 + +0.9 482,462 + -1.9 482,462 + -0.7 482,462 + -0.7 482,462 + +0.3 482,462 + +0.9 482,462 + +0.9 482,463 - -0.4 482,463 - +0.7 482,463 - +0.7 482,463 - -0.7 482,463 - +0.9 482,463 - -1.9 482,463 - +0.9 482,463 - +0.9 482,481 + -1.3 482,482 - +0.9 482,482 - -2.2 482,482 - -0.1 482,482 - +0.9 482,482 - +0.9 482,740 + Pf6N2E2_414 0.62 +0.9 482,741 - Pf6N2E2_414 0.62 +1.2 482,741 - Pf6N2E2_414 0.62 +0.9 482,741 - Pf6N2E2_414 0.62 +0.9 482,743 - Pf6N2E2_414 0.62 +0.5 482,743 - Pf6N2E2_414 0.62 +0.9 482,909 - -0.7 482,909 - +0.9 482,909 - +0.9 483,025 - Pf6N2E2_415 0.10 +0.9 483,090 + Pf6N2E2_415 0.18 -0.1 483,090 + Pf6N2E2_415 0.18 -1.1 483,090 + Pf6N2E2_415 0.18 +0.9 483,090 + Pf6N2E2_415 0.18 +0.9 483,091 - Pf6N2E2_415 0.18 -1.1 483,091 - Pf6N2E2_415 0.18 -0.1 483,091 - Pf6N2E2_415 0.18 +0.9 483,240 + Pf6N2E2_415 0.36 +0.9 483,241 - Pf6N2E2_415 0.36 -0.1 483,315 - Pf6N2E2_415 0.45 -1.2 483,315 - Pf6N2E2_415 0.45 +0.9 483,393 + Pf6N2E2_415 0.54 +0.9 483,394 - Pf6N2E2_415 0.54 -0.1 483,465 - Pf6N2E2_415 0.63 -1.3 483,465 - Pf6N2E2_415 0.63 -0.5 483,548 + Pf6N2E2_415 0.73 -0.1 483,548 + Pf6N2E2_415 0.73 -0.1 483,548 + Pf6N2E2_415 0.73 -0.7 483,548 + Pf6N2E2_415 0.73 -0.5 483,548 + Pf6N2E2_415 0.73 +0.9 483,548 + Pf6N2E2_415 0.73 -2.2 483,549 - Pf6N2E2_415 0.73 +0.9 483,549 - Pf6N2E2_415 0.73 -0.7 483,549 - Pf6N2E2_415 0.73 -0.1 483,588 + Pf6N2E2_415 0.78 +0.9 483,597 + Pf6N2E2_415 0.79 -1.0 483,597 + Pf6N2E2_415 0.79 -0.7 483,598 - Pf6N2E2_415 0.79 +0.9 483,612 + Pf6N2E2_415 0.80 +0.9 483,613 - Pf6N2E2_415 0.81 +0.9 483,691 - Pf6N2E2_415 0.90 +0.9 483,691 - Pf6N2E2_415 0.90 +0.9 483,859 + Pf6N2E2_416 0.16 -0.1 483,859 + Pf6N2E2_416 0.16 +0.9
Or see this region's nucleotide sequence