Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3740

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3738 and Pf6N2E2_3739 overlap by 4 nucleotidesPf6N2E2_3739 and Pf6N2E2_3740 overlap by 4 nucleotidesPf6N2E2_3740 and Pf6N2E2_3741 overlap by 8 nucleotidesPf6N2E2_3741 and Pf6N2E2_3742 overlap by 8 nucleotidesPf6N2E2_3742 and Pf6N2E2_3743 are separated by 2 nucleotides Pf6N2E2_3738 - General secretion pathway protein L, at 4,354,377 to 4,355,480 _3738 Pf6N2E2_3739 - General secretion pathway protein K, at 4,355,477 to 4,356,325 _3739 Pf6N2E2_3740 - General secretion pathway protein J, at 4,356,322 to 4,356,855 _3740 Pf6N2E2_3741 - General secretion pathway protein I, at 4,356,848 to 4,357,234 _3741 Pf6N2E2_3742 - General secretion pathway protein H, at 4,357,227 to 4,357,694 _3742 Pf6N2E2_3743 - General secretion pathway protein G, at 4,357,697 to 4,358,152 _3743 Position (kb) 4356 4357Strain fitness (log2 ratio) -3 -2 -1 0 1at 4355.464 kb on + strandat 4355.798 kb on - strand, within Pf6N2E2_3739at 4355.933 kb on + strand, within Pf6N2E2_3739at 4356.010 kb on + strand, within Pf6N2E2_3739at 4356.010 kb on + strand, within Pf6N2E2_3739at 4356.011 kb on - strand, within Pf6N2E2_3739at 4356.110 kb on + strand, within Pf6N2E2_3739at 4356.111 kb on - strand, within Pf6N2E2_3739at 4356.128 kb on + strand, within Pf6N2E2_3739at 4356.129 kb on - strand, within Pf6N2E2_3739at 4356.143 kb on + strand, within Pf6N2E2_3739at 4356.144 kb on - strand, within Pf6N2E2_3739at 4356.144 kb on - strand, within Pf6N2E2_3739at 4356.455 kb on - strand, within Pf6N2E2_3740at 4356.455 kb on - strand, within Pf6N2E2_3740at 4356.455 kb on - strand, within Pf6N2E2_3740at 4356.475 kb on + strand, within Pf6N2E2_3740at 4356.475 kb on + strand, within Pf6N2E2_3740at 4356.475 kb on + strand, within Pf6N2E2_3740at 4356.476 kb on - strand, within Pf6N2E2_3740at 4356.476 kb on - strand, within Pf6N2E2_3740at 4356.615 kb on + strand, within Pf6N2E2_3740at 4356.616 kb on - strand, within Pf6N2E2_3740at 4356.849 kb on + strandat 4356.849 kb on + strandat 4356.849 kb on + strandat 4356.850 kb on - strandat 4357.491 kb on + strand, within Pf6N2E2_3742at 4357.491 kb on + strand, within Pf6N2E2_3742at 4357.491 kb on + strand, within Pf6N2E2_3742at 4357.491 kb on + strand, within Pf6N2E2_3742at 4357.497 kb on + strand, within Pf6N2E2_3742at 4357.498 kb on - strand, within Pf6N2E2_3742at 4357.498 kb on - strand, within Pf6N2E2_3742at 4357.504 kb on + strand, within Pf6N2E2_3742at 4357.767 kb on + strand, within Pf6N2E2_3743at 4357.768 kb on - strand, within Pf6N2E2_3743at 4357.768 kb on - strand, within Pf6N2E2_3743at 4357.818 kb on + strand, within Pf6N2E2_3743

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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4,355,464 + -0.4
4,355,798 - Pf6N2E2_3739 0.38 +1.0
4,355,933 + Pf6N2E2_3739 0.54 +1.0
4,356,010 + Pf6N2E2_3739 0.63 +1.0
4,356,010 + Pf6N2E2_3739 0.63 +0.0
4,356,011 - Pf6N2E2_3739 0.63 +1.0
4,356,110 + Pf6N2E2_3739 0.75 -0.5
4,356,111 - Pf6N2E2_3739 0.75 -0.6
4,356,128 + Pf6N2E2_3739 0.77 +0.0
4,356,129 - Pf6N2E2_3739 0.77 +0.0
4,356,143 + Pf6N2E2_3739 0.78 +0.0
4,356,144 - Pf6N2E2_3739 0.79 -0.6
4,356,144 - Pf6N2E2_3739 0.79 -1.0
4,356,455 - Pf6N2E2_3740 0.25 -2.8
4,356,455 - Pf6N2E2_3740 0.25 +1.0
4,356,455 - Pf6N2E2_3740 0.25 -0.3
4,356,475 + Pf6N2E2_3740 0.29 -0.6
4,356,475 + Pf6N2E2_3740 0.29 +1.0
4,356,475 + Pf6N2E2_3740 0.29 +0.0
4,356,476 - Pf6N2E2_3740 0.29 +0.0
4,356,476 - Pf6N2E2_3740 0.29 -0.6
4,356,615 + Pf6N2E2_3740 0.55 +1.0
4,356,616 - Pf6N2E2_3740 0.55 -1.5
4,356,849 + +0.0
4,356,849 + +0.0
4,356,849 + +1.0
4,356,850 - -1.0
4,357,491 + Pf6N2E2_3742 0.56 +0.0
4,357,491 + Pf6N2E2_3742 0.56 +1.0
4,357,491 + Pf6N2E2_3742 0.56 +1.0
4,357,491 + Pf6N2E2_3742 0.56 -1.8
4,357,497 + Pf6N2E2_3742 0.58 -0.6
4,357,498 - Pf6N2E2_3742 0.58 +0.0
4,357,498 - Pf6N2E2_3742 0.58 +1.0
4,357,504 + Pf6N2E2_3742 0.59 -0.3
4,357,767 + Pf6N2E2_3743 0.15 +0.0
4,357,768 - Pf6N2E2_3743 0.16 +1.0
4,357,768 - Pf6N2E2_3743 0.16 +0.7
4,357,818 + Pf6N2E2_3743 0.27 +1.0

Or see this region's nucleotide sequence