Experiment: Ying_AminoAcid20 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1613 and Pf6N2E2_1614 are separated by 221 nucleotides Pf6N2E2_1614 and Pf6N2E2_1615 are separated by 131 nucleotides
Pf6N2E2_1613 - short-chain dehydrogenase/reductase, at 1,886,760 to 1,887,503
_1613
Pf6N2E2_1614 - Transcriptional regulator, LysR family, at 1,887,725 to 1,888,630
_1614
Pf6N2E2_1615 - Aldo-keto reductase, at 1,888,762 to 1,889,757
_1615
Position (kb) 1887
1888
1889 Strain fitness (log2 ratio) -1
0
1
2 at 1886.745 kb on + strand at 1886.766 kb on - strand at 1886.787 kb on - strand at 1886.791 kb on + strand at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.201 kb on + strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.202 kb on - strand, within Pf6N2E2_1613 at 1887.460 kb on - strand at 1887.460 kb on - strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.489 kb on + strand at 1887.491 kb on + strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.492 kb on - strand at 1887.521 kb on + strand at 1887.521 kb on + strand at 1887.522 kb on - strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.533 kb on + strand at 1887.534 kb on - strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.551 kb on + strand at 1887.591 kb on + strand at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.879 kb on - strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.965 kb on + strand, within Pf6N2E2_1614 at 1887.966 kb on - strand, within Pf6N2E2_1614 at 1887.969 kb on + strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1887.970 kb on - strand, within Pf6N2E2_1614 at 1888.208 kb on - strand, within Pf6N2E2_1614 at 1888.336 kb on + strand, within Pf6N2E2_1614 at 1888.452 kb on + strand, within Pf6N2E2_1614 at 1888.453 kb on - strand, within Pf6N2E2_1614 at 1888.456 kb on - strand, within Pf6N2E2_1614 at 1888.512 kb on - strand, within Pf6N2E2_1614 at 1888.638 kb on - strand at 1888.667 kb on - strand at 1888.670 kb on + strand at 1888.723 kb on - strand at 1888.755 kb on + strand at 1888.756 kb on - strand at 1888.756 kb on - strand at 1888.756 kb on - strand at 1888.756 kb on - strand at 1888.840 kb on + strand at 1888.841 kb on - strand at 1888.930 kb on + strand, within Pf6N2E2_1615 at 1888.930 kb on + strand, within Pf6N2E2_1615 at 1888.930 kb on + strand, within Pf6N2E2_1615 at 1888.930 kb on + strand, within Pf6N2E2_1615 at 1888.930 kb on + strand, within Pf6N2E2_1615 at 1888.931 kb on - strand, within Pf6N2E2_1615 at 1888.941 kb on + strand, within Pf6N2E2_1615 at 1889.065 kb on + strand, within Pf6N2E2_1615 at 1889.066 kb on - strand, within Pf6N2E2_1615 at 1889.113 kb on + strand, within Pf6N2E2_1615 at 1889.125 kb on + strand, within Pf6N2E2_1615 at 1889.126 kb on - strand, within Pf6N2E2_1615 at 1889.128 kb on + strand, within Pf6N2E2_1615 at 1889.128 kb on + strand, within Pf6N2E2_1615 at 1889.128 kb on + strand, within Pf6N2E2_1615 at 1889.338 kb on - strand, within Pf6N2E2_1615
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2 remove 1,886,745 + +0.9 1,886,766 - -0.1 1,886,787 - +0.9 1,886,791 + +0.9 1,887,201 + Pf6N2E2_1613 0.59 +0.0 1,887,201 + Pf6N2E2_1613 0.59 -0.7 1,887,202 - Pf6N2E2_1613 0.59 -0.6 1,887,202 - Pf6N2E2_1613 0.59 -0.1 1,887,202 - Pf6N2E2_1613 0.59 -0.3 1,887,460 - -0.1 1,887,460 - -0.1 1,887,489 + -0.5 1,887,489 + +1.9 1,887,489 + +0.9 1,887,491 + +0.9 1,887,492 - -0.1 1,887,492 - +0.9 1,887,492 - -0.1 1,887,521 + -0.1 1,887,521 + -0.7 1,887,522 - +0.9 1,887,533 + +0.9 1,887,533 + +0.9 1,887,533 + +0.9 1,887,534 - -0.4 1,887,551 + -1.7 1,887,551 + +0.9 1,887,551 + -1.4 1,887,591 + +0.9 1,887,879 - Pf6N2E2_1614 0.17 +0.9 1,887,879 - Pf6N2E2_1614 0.17 -0.7 1,887,965 + Pf6N2E2_1614 0.26 +0.9 1,887,965 + Pf6N2E2_1614 0.26 +0.9 1,887,966 - Pf6N2E2_1614 0.27 +0.9 1,887,969 + Pf6N2E2_1614 0.27 +0.9 1,887,970 - Pf6N2E2_1614 0.27 -0.7 1,887,970 - Pf6N2E2_1614 0.27 -0.7 1,887,970 - Pf6N2E2_1614 0.27 -0.1 1,888,208 - Pf6N2E2_1614 0.53 +0.9 1,888,336 + Pf6N2E2_1614 0.67 +0.9 1,888,452 + Pf6N2E2_1614 0.80 +0.9 1,888,453 - Pf6N2E2_1614 0.80 -1.3 1,888,456 - Pf6N2E2_1614 0.81 -0.1 1,888,512 - Pf6N2E2_1614 0.87 +0.9 1,888,638 - -0.6 1,888,667 - -0.1 1,888,670 + +0.9 1,888,723 - -0.1 1,888,755 + -0.5 1,888,756 - +0.9 1,888,756 - -1.5 1,888,756 - +0.9 1,888,756 - +0.9 1,888,840 + +0.9 1,888,841 - +1.9 1,888,930 + Pf6N2E2_1615 0.17 -0.3 1,888,930 + Pf6N2E2_1615 0.17 -1.1 1,888,930 + Pf6N2E2_1615 0.17 +0.9 1,888,930 + Pf6N2E2_1615 0.17 -0.1 1,888,930 + Pf6N2E2_1615 0.17 +0.9 1,888,931 - Pf6N2E2_1615 0.17 -0.1 1,888,941 + Pf6N2E2_1615 0.18 +0.9 1,889,065 + Pf6N2E2_1615 0.30 +0.9 1,889,066 - Pf6N2E2_1615 0.31 -0.1 1,889,113 + Pf6N2E2_1615 0.35 -0.2 1,889,125 + Pf6N2E2_1615 0.36 -0.1 1,889,126 - Pf6N2E2_1615 0.37 +0.9 1,889,128 + Pf6N2E2_1615 0.37 +0.9 1,889,128 + Pf6N2E2_1615 0.37 -0.1 1,889,128 + Pf6N2E2_1615 0.37 +0.9 1,889,338 - Pf6N2E2_1615 0.58 +0.9
Or see this region's nucleotide sequence