Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1523

Experiment: Ying_AminoAcid20 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1522 and Pf6N2E2_1523 overlap by 11 nucleotidesPf6N2E2_1523 and Pf6N2E2_1524 are separated by 246 nucleotidesPf6N2E2_1524 and Pf6N2E2_1525 are separated by 51 nucleotides Pf6N2E2_1522 - Alcohol dehydrogenase (EC 1.1.1.1), at 1,780,102 to 1,781,085 _1522 Pf6N2E2_1523 - Universal stress protein family 5, at 1,781,075 to 1,781,968 _1523 Pf6N2E2_1524 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 1,782,215 to 1,782,778 _1524 Pf6N2E2_1525 - Universal stress protein family 5, at 1,782,830 to 1,783,741 _1525 Position (kb) 1781 1782Strain fitness (log2 ratio) -2 -1 0 1at 1780.465 kb on + strand, within Pf6N2E2_1522at 1780.604 kb on - strand, within Pf6N2E2_1522at 1780.666 kb on + strand, within Pf6N2E2_1522at 1780.666 kb on + strand, within Pf6N2E2_1522at 1780.666 kb on + strand, within Pf6N2E2_1522at 1780.666 kb on + strand, within Pf6N2E2_1522at 1780.667 kb on - strand, within Pf6N2E2_1522at 1781.006 kb on - strandat 1781.072 kb on + strandat 1781.073 kb on - strandat 1781.073 kb on - strandat 1781.112 kb on + strandat 1781.112 kb on + strandat 1781.113 kb on - strandat 1781.290 kb on + strand, within Pf6N2E2_1523at 1781.291 kb on - strand, within Pf6N2E2_1523at 1781.397 kb on + strand, within Pf6N2E2_1523at 1781.398 kb on - strand, within Pf6N2E2_1523at 1781.626 kb on + strand, within Pf6N2E2_1523at 1781.703 kb on - strand, within Pf6N2E2_1523at 1781.822 kb on + strand, within Pf6N2E2_1523at 1781.822 kb on + strand, within Pf6N2E2_1523at 1781.822 kb on + strand, within Pf6N2E2_1523at 1781.823 kb on - strand, within Pf6N2E2_1523at 1781.823 kb on - strand, within Pf6N2E2_1523at 1781.946 kb on - strandat 1782.476 kb on + strand, within Pf6N2E2_1524at 1782.476 kb on + strand, within Pf6N2E2_1524at 1782.476 kb on + strand, within Pf6N2E2_1524at 1782.477 kb on - strand, within Pf6N2E2_1524at 1782.477 kb on - strand, within Pf6N2E2_1524at 1782.477 kb on - strand, within Pf6N2E2_1524at 1782.662 kb on + strand, within Pf6N2E2_1524at 1782.662 kb on + strand, within Pf6N2E2_1524at 1782.663 kb on - strand, within Pf6N2E2_1524at 1782.663 kb on - strand, within Pf6N2E2_1524at 1782.771 kb on - strandat 1782.805 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 2
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1,780,465 + Pf6N2E2_1522 0.37 -0.4
1,780,604 - Pf6N2E2_1522 0.51 -0.1
1,780,666 + Pf6N2E2_1522 0.57 -1.1
1,780,666 + Pf6N2E2_1522 0.57 +0.1
1,780,666 + Pf6N2E2_1522 0.57 -0.4
1,780,666 + Pf6N2E2_1522 0.57 +0.9
1,780,667 - Pf6N2E2_1522 0.57 -0.4
1,781,006 - -0.1
1,781,072 + +0.9
1,781,073 - -0.7
1,781,073 - -0.5
1,781,112 + +0.9
1,781,112 + +0.4
1,781,113 - +0.9
1,781,290 + Pf6N2E2_1523 0.24 -0.1
1,781,291 - Pf6N2E2_1523 0.24 -1.1
1,781,397 + Pf6N2E2_1523 0.36 -1.9
1,781,398 - Pf6N2E2_1523 0.36 -0.1
1,781,626 + Pf6N2E2_1523 0.62 +0.9
1,781,703 - Pf6N2E2_1523 0.70 +0.9
1,781,822 + Pf6N2E2_1523 0.84 -0.1
1,781,822 + Pf6N2E2_1523 0.84 +0.9
1,781,822 + Pf6N2E2_1523 0.84 +0.9
1,781,823 - Pf6N2E2_1523 0.84 +0.9
1,781,823 - Pf6N2E2_1523 0.84 -0.1
1,781,946 - +0.9
1,782,476 + Pf6N2E2_1524 0.46 -0.6
1,782,476 + Pf6N2E2_1524 0.46 +0.9
1,782,476 + Pf6N2E2_1524 0.46 +0.9
1,782,477 - Pf6N2E2_1524 0.46 +0.9
1,782,477 - Pf6N2E2_1524 0.46 -1.1
1,782,477 - Pf6N2E2_1524 0.46 -1.7
1,782,662 + Pf6N2E2_1524 0.79 +0.1
1,782,662 + Pf6N2E2_1524 0.79 -0.1
1,782,663 - Pf6N2E2_1524 0.79 -0.1
1,782,663 - Pf6N2E2_1524 0.79 +0.9
1,782,771 - -0.7
1,782,805 - -0.1

Or see this region's nucleotide sequence