Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_798 and Pf6N2E2_799 overlap by 48 nucleotides Pf6N2E2_799 and Pf6N2E2_800 are separated by 49 nucleotides Pf6N2E2_800 and Pf6N2E2_801 are separated by 47 nucleotides Pf6N2E2_801 and Pf6N2E2_802 are separated by 342 nucleotides
Pf6N2E2_798 - Ku domain protein, at 930,292 to 931,182
_798
Pf6N2E2_799 - hypothetical protein, at 931,135 to 931,344
_799
Pf6N2E2_800 - FIG00962948: hypothetical protein, at 931,394 to 931,723
_800
Pf6N2E2_801 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 931,771 to 932,919
_801
Pf6N2E2_802 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 933,262 to 934,269
_802
Position (kb) 931
932
933 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 930.815 kb on + strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.816 kb on - strand, within Pf6N2E2_798 at 930.968 kb on + strand, within Pf6N2E2_798 at 930.968 kb on + strand, within Pf6N2E2_798 at 931.295 kb on + strand, within Pf6N2E2_799 at 931.295 kb on + strand, within Pf6N2E2_799 at 931.320 kb on + strand, within Pf6N2E2_799 at 931.414 kb on - strand at 931.414 kb on - strand at 931.414 kb on - strand at 931.416 kb on + strand at 931.416 kb on + strand at 931.416 kb on + strand at 931.417 kb on - strand at 931.417 kb on - strand at 931.499 kb on + strand, within Pf6N2E2_800 at 931.499 kb on + strand, within Pf6N2E2_800 at 931.499 kb on + strand, within Pf6N2E2_800 at 931.500 kb on - strand, within Pf6N2E2_800 at 931.500 kb on - strand, within Pf6N2E2_800 at 931.501 kb on + strand, within Pf6N2E2_800 at 931.502 kb on - strand, within Pf6N2E2_800 at 931.502 kb on - strand, within Pf6N2E2_800 at 931.566 kb on + strand, within Pf6N2E2_800 at 931.566 kb on + strand, within Pf6N2E2_800 at 931.566 kb on + strand, within Pf6N2E2_800 at 931.566 kb on + strand, within Pf6N2E2_800 at 931.566 kb on + strand, within Pf6N2E2_800 at 931.567 kb on - strand, within Pf6N2E2_800 at 931.567 kb on - strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.570 kb on + strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.571 kb on - strand, within Pf6N2E2_800 at 931.779 kb on + strand at 931.779 kb on + strand at 931.780 kb on - strand at 931.780 kb on - strand at 931.780 kb on - strand at 932.107 kb on + strand, within Pf6N2E2_801 at 932.108 kb on - strand, within Pf6N2E2_801 at 932.108 kb on - strand, within Pf6N2E2_801 at 932.108 kb on - strand, within Pf6N2E2_801 at 932.117 kb on - strand, within Pf6N2E2_801 at 932.117 kb on - strand, within Pf6N2E2_801 at 932.281 kb on + strand, within Pf6N2E2_801 at 932.282 kb on - strand, within Pf6N2E2_801 at 932.292 kb on + strand, within Pf6N2E2_801 at 932.292 kb on + strand, within Pf6N2E2_801 at 932.293 kb on - strand, within Pf6N2E2_801 at 932.293 kb on - strand, within Pf6N2E2_801 at 932.293 kb on - strand, within Pf6N2E2_801 at 932.365 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.429 kb on + strand, within Pf6N2E2_801 at 932.430 kb on - strand, within Pf6N2E2_801 at 932.430 kb on - strand, within Pf6N2E2_801 at 932.430 kb on - strand, within Pf6N2E2_801 at 932.430 kb on - strand, within Pf6N2E2_801 at 932.554 kb on + strand, within Pf6N2E2_801 at 932.554 kb on + strand, within Pf6N2E2_801 at 932.554 kb on + strand, within Pf6N2E2_801 at 932.555 kb on - strand, within Pf6N2E2_801 at 932.587 kb on + strand, within Pf6N2E2_801 at 932.587 kb on + strand, within Pf6N2E2_801 at 932.588 kb on - strand, within Pf6N2E2_801 at 932.692 kb on + strand, within Pf6N2E2_801 at 932.692 kb on + strand, within Pf6N2E2_801 at 932.692 kb on + strand, within Pf6N2E2_801 at 932.860 kb on + strand at 932.860 kb on + strand at 932.861 kb on - strand at 932.866 kb on + strand at 932.866 kb on + strand at 932.866 kb on + strand at 932.866 kb on + strand at 932.866 kb on + strand at 932.866 kb on + strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.867 kb on - strand at 932.899 kb on + strand at 932.899 kb on + strand at 932.899 kb on + strand at 932.917 kb on + strand at 932.958 kb on + strand at 932.978 kb on + strand at 932.978 kb on + strand at 933.072 kb on + strand at 933.073 kb on - strand at 933.152 kb on - strand at 933.152 kb on - strand at 933.152 kb on - strand at 933.152 kb on - strand at 933.167 kb on + strand at 933.167 kb on + strand at 933.168 kb on - strand at 933.168 kb on - strand at 933.180 kb on - strand at 933.301 kb on + strand at 933.301 kb on + strand at 933.301 kb on + strand at 933.301 kb on + strand at 933.302 kb on - strand at 933.302 kb on - strand at 933.302 kb on - strand at 933.302 kb on - strand at 933.302 kb on - strand at 933.302 kb on - strand at 933.306 kb on + strand at 933.306 kb on + strand at 933.306 kb on + strand at 933.307 kb on - strand at 933.331 kb on + strand at 933.331 kb on + strand at 933.332 kb on - strand at 933.332 kb on - strand at 933.454 kb on + strand, within Pf6N2E2_802 at 933.455 kb on - strand, within Pf6N2E2_802 at 933.455 kb on - strand, within Pf6N2E2_802 at 933.455 kb on - strand, within Pf6N2E2_802 at 933.561 kb on + strand, within Pf6N2E2_802 at 933.562 kb on - strand, within Pf6N2E2_802 at 933.853 kb on + strand, within Pf6N2E2_802 at 933.854 kb on - strand, within Pf6N2E2_802 at 933.854 kb on - strand, within Pf6N2E2_802 at 933.862 kb on + strand, within Pf6N2E2_802 at 933.863 kb on - strand, within Pf6N2E2_802 at 933.878 kb on + strand, within Pf6N2E2_802 at 933.879 kb on - strand, within Pf6N2E2_802 at 933.879 kb on - strand, within Pf6N2E2_802 at 933.879 kb on - strand, within Pf6N2E2_802
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 930,815 + Pf6N2E2_798 0.59 -1.0 930,816 - Pf6N2E2_798 0.59 -0.1 930,816 - Pf6N2E2_798 0.59 +0.9 930,816 - Pf6N2E2_798 0.59 -0.1 930,816 - Pf6N2E2_798 0.59 -0.3 930,968 + Pf6N2E2_798 0.76 +0.9 930,968 + Pf6N2E2_798 0.76 +0.9 931,295 + Pf6N2E2_799 0.76 +0.9 931,295 + Pf6N2E2_799 0.76 +0.9 931,320 + Pf6N2E2_799 0.88 -0.1 931,414 - -0.6 931,414 - +0.9 931,414 - -0.8 931,416 + -0.1 931,416 + +0.9 931,416 + -0.7 931,417 - +0.9 931,417 - +0.9 931,499 + Pf6N2E2_800 0.32 +0.9 931,499 + Pf6N2E2_800 0.32 +0.9 931,499 + Pf6N2E2_800 0.32 +0.9 931,500 - Pf6N2E2_800 0.32 +1.4 931,500 - Pf6N2E2_800 0.32 +0.9 931,501 + Pf6N2E2_800 0.32 -0.6 931,502 - Pf6N2E2_800 0.33 -0.3 931,502 - Pf6N2E2_800 0.33 -1.0 931,566 + Pf6N2E2_800 0.52 +0.9 931,566 + Pf6N2E2_800 0.52 -0.7 931,566 + Pf6N2E2_800 0.52 +0.9 931,566 + Pf6N2E2_800 0.52 -0.1 931,566 + Pf6N2E2_800 0.52 +0.9 931,567 - Pf6N2E2_800 0.52 +0.9 931,567 - Pf6N2E2_800 0.52 -0.4 931,570 + Pf6N2E2_800 0.53 +0.9 931,570 + Pf6N2E2_800 0.53 +0.9 931,570 + Pf6N2E2_800 0.53 -0.4 931,570 + Pf6N2E2_800 0.53 -0.5 931,570 + Pf6N2E2_800 0.53 +0.3 931,570 + Pf6N2E2_800 0.53 -0.1 931,570 + Pf6N2E2_800 0.53 -1.1 931,571 - Pf6N2E2_800 0.54 -0.7 931,571 - Pf6N2E2_800 0.54 -0.8 931,571 - Pf6N2E2_800 0.54 +0.9 931,571 - Pf6N2E2_800 0.54 -0.1 931,571 - Pf6N2E2_800 0.54 +0.9 931,571 - Pf6N2E2_800 0.54 -0.4 931,571 - Pf6N2E2_800 0.54 -0.1 931,779 + -0.1 931,779 + -0.1 931,780 - +0.9 931,780 - +0.9 931,780 - +0.9 932,107 + Pf6N2E2_801 0.29 -1.1 932,108 - Pf6N2E2_801 0.29 +0.9 932,108 - Pf6N2E2_801 0.29 -0.1 932,108 - Pf6N2E2_801 0.29 -0.7 932,117 - Pf6N2E2_801 0.30 -0.1 932,117 - Pf6N2E2_801 0.30 -1.0 932,281 + Pf6N2E2_801 0.44 +0.9 932,282 - Pf6N2E2_801 0.44 -0.7 932,292 + Pf6N2E2_801 0.45 -0.1 932,292 + Pf6N2E2_801 0.45 -0.5 932,293 - Pf6N2E2_801 0.45 -2.9 932,293 - Pf6N2E2_801 0.45 +0.9 932,293 - Pf6N2E2_801 0.45 +0.9 932,365 + Pf6N2E2_801 0.52 -0.1 932,429 + Pf6N2E2_801 0.57 +0.9 932,429 + Pf6N2E2_801 0.57 -0.9 932,429 + Pf6N2E2_801 0.57 -0.1 932,429 + Pf6N2E2_801 0.57 -0.7 932,429 + Pf6N2E2_801 0.57 +0.9 932,429 + Pf6N2E2_801 0.57 +0.9 932,430 - Pf6N2E2_801 0.57 -2.1 932,430 - Pf6N2E2_801 0.57 +1.9 932,430 - Pf6N2E2_801 0.57 -1.1 932,430 - Pf6N2E2_801 0.57 -0.6 932,554 + Pf6N2E2_801 0.68 -1.0 932,554 + Pf6N2E2_801 0.68 +1.2 932,554 + Pf6N2E2_801 0.68 -0.1 932,555 - Pf6N2E2_801 0.68 -0.6 932,587 + Pf6N2E2_801 0.71 +0.9 932,587 + Pf6N2E2_801 0.71 -0.1 932,588 - Pf6N2E2_801 0.71 +0.9 932,692 + Pf6N2E2_801 0.80 +0.9 932,692 + Pf6N2E2_801 0.80 -0.1 932,692 + Pf6N2E2_801 0.80 -0.9 932,860 + +0.9 932,860 + +0.1 932,861 - +0.9 932,866 + -1.7 932,866 + -0.1 932,866 + -0.1 932,866 + -0.1 932,866 + +0.9 932,866 + -0.4 932,867 - -0.4 932,867 - +0.9 932,867 - -0.4 932,867 - +0.9 932,867 - +0.9 932,867 - +0.9 932,867 - -0.1 932,867 - +0.9 932,867 - +0.9 932,867 - -0.1 932,899 + +0.9 932,899 + -0.9 932,899 + +0.9 932,917 + +0.2 932,958 + +0.9 932,978 + +1.9 932,978 + +0.9 933,072 + -0.4 933,073 - +0.9 933,152 - +0.2 933,152 - +0.9 933,152 - -0.7 933,152 - -0.7 933,167 + -0.7 933,167 + +0.9 933,168 - -0.7 933,168 - -0.1 933,180 - +0.9 933,301 + +0.9 933,301 + +0.5 933,301 + -1.1 933,301 + +0.9 933,302 - +0.6 933,302 - +0.9 933,302 - +0.9 933,302 - +0.9 933,302 - +0.9 933,302 - +1.9 933,306 + -0.7 933,306 + +0.9 933,306 + +0.9 933,307 - +0.9 933,331 + +0.9 933,331 + +0.9 933,332 - -0.1 933,332 - +0.9 933,454 + Pf6N2E2_802 0.19 +0.9 933,455 - Pf6N2E2_802 0.19 -0.1 933,455 - Pf6N2E2_802 0.19 -0.1 933,455 - Pf6N2E2_802 0.19 -0.1 933,561 + Pf6N2E2_802 0.30 -0.1 933,562 - Pf6N2E2_802 0.30 +0.9 933,853 + Pf6N2E2_802 0.59 -0.1 933,854 - Pf6N2E2_802 0.59 +0.2 933,854 - Pf6N2E2_802 0.59 -0.7 933,862 + Pf6N2E2_802 0.60 -0.1 933,863 - Pf6N2E2_802 0.60 -0.1 933,878 + Pf6N2E2_802 0.61 -1.1 933,879 - Pf6N2E2_802 0.61 -1.2 933,879 - Pf6N2E2_802 0.61 +0.9 933,879 - Pf6N2E2_802 0.61 -0.5
Or see this region's nucleotide sequence