Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5920 and Pf6N2E2_5921 are separated by 478 nucleotides Pf6N2E2_5921 and Pf6N2E2_5922 are separated by 90 nucleotides
Pf6N2E2_5920 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 6,738,867 to 6,740,090
_5920
Pf6N2E2_5921 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 6,740,569 to 6,741,195
_5921
Pf6N2E2_5922 - Transcriptional regulator, LysR family, at 6,741,286 to 6,742,284
_5922
Position (kb)
6740
6741
6742 Strain fitness (log2 ratio)
-2
-1
0
1 at 6739.722 kb on + strand, within Pf6N2E2_5920 at 6739.723 kb on - strand, within Pf6N2E2_5920 at 6739.789 kb on - strand, within Pf6N2E2_5920 at 6740.064 kb on + strand at 6740.064 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.120 kb on + strand at 6740.121 kb on - strand at 6740.130 kb on + strand at 6740.130 kb on + strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.149 kb on - strand at 6740.149 kb on - strand at 6740.381 kb on + strand at 6740.382 kb on - strand at 6740.426 kb on + strand at 6740.582 kb on + strand at 6740.583 kb on - strand at 6740.583 kb on - strand at 6740.596 kb on + strand at 6740.596 kb on + strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.679 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.202 kb on + strand at 6741.229 kb on + strand at 6741.230 kb on - strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.231 kb on + strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.232 kb on - strand at 6741.300 kb on - strand at 6741.300 kb on - strand at 6741.411 kb on + strand, within Pf6N2E2_5922 at 6741.411 kb on + strand, within Pf6N2E2_5922 at 6741.597 kb on - strand, within Pf6N2E2_5922 at 6741.814 kb on + strand, within Pf6N2E2_5922 at 6741.815 kb on - strand, within Pf6N2E2_5922 at 6741.815 kb on - strand, within Pf6N2E2_5922 at 6741.831 kb on + strand, within Pf6N2E2_5922 at 6741.831 kb on + strand, within Pf6N2E2_5922 at 6741.832 kb on - strand, within Pf6N2E2_5922 at 6741.874 kb on + strand, within Pf6N2E2_5922 at 6741.875 kb on - strand, within Pf6N2E2_5922 at 6741.875 kb on - strand, within Pf6N2E2_5922 at 6742.025 kb on - strand, within Pf6N2E2_5922 at 6742.071 kb on - strand, within Pf6N2E2_5922 at 6742.071 kb on - strand, within Pf6N2E2_5922 at 6742.138 kb on + strand, within Pf6N2E2_5922
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 6,739,722 + Pf6N2E2_5920 0.70 -0.1 6,739,723 - Pf6N2E2_5920 0.70 -0.6 6,739,789 - Pf6N2E2_5920 0.75 -1.1 6,740,064 + +0.9 6,740,064 + +0.9 6,740,078 + +0.9 6,740,078 + -0.1 6,740,078 + -0.1 6,740,079 - -0.6 6,740,079 - +0.9 6,740,079 - -0.6 6,740,120 + -0.3 6,740,121 - +0.9 6,740,130 + -1.1 6,740,130 + -0.6 6,740,131 - +0.9 6,740,131 - -0.1 6,740,131 - -0.1 6,740,148 + +0.9 6,740,148 + -1.1 6,740,148 + +0.9 6,740,148 + -0.1 6,740,148 + +0.9 6,740,149 - +0.9 6,740,149 - -0.2 6,740,381 + +0.9 6,740,382 - -0.1 6,740,426 + -0.7 6,740,582 + +0.9 6,740,583 - -1.0 6,740,583 - -0.7 6,740,596 + -1.4 6,740,596 + -0.1 6,740,597 - -0.6 6,740,597 - -1.1 6,740,597 - +0.5 6,740,679 - Pf6N2E2_5921 0.18 -1.1 6,740,825 - Pf6N2E2_5921 0.41 -1.3 6,740,825 - Pf6N2E2_5921 0.41 -0.1 6,740,990 + Pf6N2E2_5921 0.67 +0.9 6,740,990 + Pf6N2E2_5921 0.67 -0.6 6,740,990 + Pf6N2E2_5921 0.67 +0.9 6,740,991 - Pf6N2E2_5921 0.67 +0.9 6,740,991 - Pf6N2E2_5921 0.67 -1.4 6,741,025 + Pf6N2E2_5921 0.73 -0.1 6,741,025 + Pf6N2E2_5921 0.73 +0.9 6,741,025 + Pf6N2E2_5921 0.73 -0.6 6,741,202 + +0.1 6,741,229 + +0.9 6,741,230 - -0.1 6,741,231 + -0.6 6,741,231 + -0.5 6,741,231 + -0.1 6,741,231 + +0.9 6,741,231 + +0.9 6,741,231 + +0.8 6,741,231 + -0.1 6,741,231 + -1.1 6,741,231 + +0.9 6,741,231 + +0.9 6,741,231 + +0.2 6,741,232 - -1.4 6,741,232 - -0.1 6,741,232 - -0.6 6,741,232 - -1.4 6,741,232 - -0.1 6,741,232 - +0.9 6,741,232 - +0.9 6,741,300 - -0.1 6,741,300 - +0.9 6,741,411 + Pf6N2E2_5922 0.13 +0.9 6,741,411 + Pf6N2E2_5922 0.13 +0.4 6,741,597 - Pf6N2E2_5922 0.31 -0.1 6,741,814 + Pf6N2E2_5922 0.53 -1.4 6,741,815 - Pf6N2E2_5922 0.53 -1.8 6,741,815 - Pf6N2E2_5922 0.53 +0.9 6,741,831 + Pf6N2E2_5922 0.55 -0.6 6,741,831 + Pf6N2E2_5922 0.55 +0.9 6,741,832 - Pf6N2E2_5922 0.55 -1.6 6,741,874 + Pf6N2E2_5922 0.59 +0.9 6,741,875 - Pf6N2E2_5922 0.59 +0.9 6,741,875 - Pf6N2E2_5922 0.59 +0.9 6,742,025 - Pf6N2E2_5922 0.74 -1.4 6,742,071 - Pf6N2E2_5922 0.79 +0.9 6,742,071 - Pf6N2E2_5922 0.79 -0.8 6,742,138 + Pf6N2E2_5922 0.85 +0.6
Or see this region's nucleotide sequence