Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4714 and Pf6N2E2_4715 are separated by 153 nucleotides Pf6N2E2_4715 and Pf6N2E2_4716 are separated by 195 nucleotides
Pf6N2E2_4714 - GbcB Glycine betaine demethylase subunit B, at 5,450,351 to 5,451,517
_4714
Pf6N2E2_4715 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 5,451,671 to 5,452,711
_4715
Pf6N2E2_4716 - Membrane protein, putative, at 5,452,907 to 5,453,611
_4716
Position (kb) 5451
5452
5453 Strain fitness (log2 ratio) -2
-1
0
1 at 5450.798 kb on + strand, within Pf6N2E2_4714 at 5450.798 kb on + strand, within Pf6N2E2_4714 at 5450.798 kb on + strand, within Pf6N2E2_4714 at 5450.852 kb on + strand, within Pf6N2E2_4714 at 5450.852 kb on + strand, within Pf6N2E2_4714 at 5450.853 kb on - strand, within Pf6N2E2_4714 at 5450.884 kb on + strand, within Pf6N2E2_4714 at 5450.885 kb on - strand, within Pf6N2E2_4714 at 5450.918 kb on + strand, within Pf6N2E2_4714 at 5450.918 kb on + strand, within Pf6N2E2_4714 at 5450.918 kb on + strand, within Pf6N2E2_4714 at 5450.919 kb on - strand, within Pf6N2E2_4714 at 5450.919 kb on - strand, within Pf6N2E2_4714 at 5450.919 kb on - strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.101 kb on + strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.102 kb on - strand, within Pf6N2E2_4714 at 5451.179 kb on - strand, within Pf6N2E2_4714 at 5451.464 kb on + strand at 5451.465 kb on - strand at 5451.568 kb on + strand at 5451.673 kb on - strand at 5451.714 kb on - strand at 5451.778 kb on - strand, within Pf6N2E2_4715 at 5451.778 kb on - strand, within Pf6N2E2_4715 at 5451.778 kb on - strand, within Pf6N2E2_4715 at 5452.027 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.258 kb on - strand, within Pf6N2E2_4715 at 5452.476 kb on + strand, within Pf6N2E2_4715 at 5452.608 kb on + strand at 5452.674 kb on + strand at 5452.674 kb on + strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.729 kb on - strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.902 kb on + strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.903 kb on - strand at 5452.983 kb on - strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.009 kb on + strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.010 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.119 kb on - strand, within Pf6N2E2_4716 at 5453.145 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.272 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.364 kb on - strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.420 kb on + strand, within Pf6N2E2_4716 at 5453.421 kb on - strand, within Pf6N2E2_4716 at 5453.552 kb on + strand at 5453.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 5,450,798 + Pf6N2E2_4714 0.38 +1.0 5,450,798 + Pf6N2E2_4714 0.38 +1.0 5,450,798 + Pf6N2E2_4714 0.38 -1.0 5,450,852 + Pf6N2E2_4714 0.43 +0.0 5,450,852 + Pf6N2E2_4714 0.43 -1.8 5,450,853 - Pf6N2E2_4714 0.43 -0.3 5,450,884 + Pf6N2E2_4714 0.46 +1.0 5,450,885 - Pf6N2E2_4714 0.46 +0.0 5,450,918 + Pf6N2E2_4714 0.49 +1.0 5,450,918 + Pf6N2E2_4714 0.49 -1.0 5,450,918 + Pf6N2E2_4714 0.49 +1.0 5,450,919 - Pf6N2E2_4714 0.49 -1.3 5,450,919 - Pf6N2E2_4714 0.49 +0.0 5,450,919 - Pf6N2E2_4714 0.49 -0.0 5,451,101 + Pf6N2E2_4714 0.64 +1.0 5,451,101 + Pf6N2E2_4714 0.64 -0.5 5,451,101 + Pf6N2E2_4714 0.64 -1.0 5,451,101 + Pf6N2E2_4714 0.64 +1.0 5,451,101 + Pf6N2E2_4714 0.64 +0.0 5,451,101 + Pf6N2E2_4714 0.64 +0.0 5,451,101 + Pf6N2E2_4714 0.64 +0.0 5,451,101 + Pf6N2E2_4714 0.64 +1.0 5,451,101 + Pf6N2E2_4714 0.64 +0.0 5,451,101 + Pf6N2E2_4714 0.64 -0.2 5,451,102 - Pf6N2E2_4714 0.64 -0.6 5,451,102 - Pf6N2E2_4714 0.64 +1.0 5,451,102 - Pf6N2E2_4714 0.64 +1.0 5,451,102 - Pf6N2E2_4714 0.64 +0.0 5,451,102 - Pf6N2E2_4714 0.64 -0.3 5,451,102 - Pf6N2E2_4714 0.64 +1.0 5,451,179 - Pf6N2E2_4714 0.71 +0.0 5,451,464 + +0.0 5,451,465 - -0.6 5,451,568 + -0.8 5,451,673 - +0.2 5,451,714 - +0.0 5,451,778 - Pf6N2E2_4715 0.10 -0.4 5,451,778 - Pf6N2E2_4715 0.10 +0.0 5,451,778 - Pf6N2E2_4715 0.10 +1.0 5,452,027 - Pf6N2E2_4715 0.34 +0.0 5,452,258 - Pf6N2E2_4715 0.56 +1.0 5,452,258 - Pf6N2E2_4715 0.56 +0.0 5,452,476 + Pf6N2E2_4715 0.77 -0.6 5,452,608 + -1.0 5,452,674 + -2.0 5,452,674 + -1.0 5,452,729 - +0.0 5,452,729 - -2.0 5,452,729 - +1.0 5,452,729 - +1.0 5,452,902 + +1.0 5,452,902 + -0.6 5,452,902 + +0.0 5,452,902 + -1.3 5,452,903 - +0.0 5,452,903 - +1.0 5,452,903 - -1.3 5,452,983 - Pf6N2E2_4716 0.11 +0.0 5,453,009 + Pf6N2E2_4716 0.14 -0.1 5,453,009 + Pf6N2E2_4716 0.14 +0.0 5,453,009 + Pf6N2E2_4716 0.14 +1.0 5,453,009 + Pf6N2E2_4716 0.14 -0.2 5,453,009 + Pf6N2E2_4716 0.14 +0.0 5,453,010 - Pf6N2E2_4716 0.15 +1.0 5,453,010 - Pf6N2E2_4716 0.15 -0.6 5,453,010 - Pf6N2E2_4716 0.15 -0.1 5,453,010 - Pf6N2E2_4716 0.15 -0.3 5,453,010 - Pf6N2E2_4716 0.15 -2.0 5,453,010 - Pf6N2E2_4716 0.15 +0.0 5,453,010 - Pf6N2E2_4716 0.15 -1.3 5,453,010 - Pf6N2E2_4716 0.15 +1.0 5,453,119 - Pf6N2E2_4716 0.30 +1.0 5,453,119 - Pf6N2E2_4716 0.30 +1.0 5,453,145 - Pf6N2E2_4716 0.34 +0.2 5,453,272 - Pf6N2E2_4716 0.52 +1.0 5,453,272 - Pf6N2E2_4716 0.52 +0.0 5,453,364 - Pf6N2E2_4716 0.65 +0.7 5,453,364 - Pf6N2E2_4716 0.65 +0.0 5,453,420 + Pf6N2E2_4716 0.73 -0.4 5,453,420 + Pf6N2E2_4716 0.73 +1.0 5,453,420 + Pf6N2E2_4716 0.73 +1.0 5,453,421 - Pf6N2E2_4716 0.73 -0.4 5,453,552 + +1.0 5,453,673 - -1.0
Or see this region's nucleotide sequence