Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4685 and Pf6N2E2_4686 are separated by 574 nucleotides Pf6N2E2_4686 and Pf6N2E2_4687 are separated by 144 nucleotides Pf6N2E2_4687 and Pf6N2E2_4688 are separated by 300 nucleotides
Pf6N2E2_4685 - L-serine dehydratase (EC 4.3.1.17), at 5,418,363 to 5,419,739
_4685
Pf6N2E2_4686 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,420,314 to 5,421,417
_4686
Pf6N2E2_4687 - FIG00953780: hypothetical protein, at 5,421,562 to 5,421,825
_4687
Pf6N2E2_4688 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 5,422,126 to 5,423,289
_4688
Position (kb) 5420
5421
5422 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5419.317 kb on + strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.318 kb on - strand, within Pf6N2E2_4685 at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.689 kb on + strand at 5419.690 kb on - strand at 5419.690 kb on - strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.691 kb on + strand at 5419.692 kb on - strand at 5419.887 kb on - strand at 5419.887 kb on - strand at 5419.898 kb on + strand at 5419.899 kb on - strand at 5419.899 kb on - strand at 5419.933 kb on + strand at 5419.934 kb on - strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.017 kb on + strand at 5420.018 kb on - strand at 5420.018 kb on - strand at 5420.059 kb on - strand at 5420.130 kb on + strand at 5420.130 kb on + strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.131 kb on - strand at 5420.141 kb on + strand at 5420.142 kb on - strand at 5420.171 kb on + strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.172 kb on - strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.217 kb on + strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.218 kb on - strand at 5420.252 kb on + strand at 5420.307 kb on + strand at 5420.307 kb on + strand at 5420.417 kb on + strand at 5420.426 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.459 kb on + strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.460 kb on - strand, within Pf6N2E2_4686 at 5420.461 kb on + strand, within Pf6N2E2_4686 at 5420.462 kb on - strand, within Pf6N2E2_4686 at 5420.562 kb on + strand, within Pf6N2E2_4686 at 5420.599 kb on - strand, within Pf6N2E2_4686 at 5420.781 kb on + strand, within Pf6N2E2_4686 at 5420.915 kb on - strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.058 kb on + strand, within Pf6N2E2_4686 at 5421.059 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.104 kb on - strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.128 kb on - strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.207 kb on - strand, within Pf6N2E2_4686 at 5421.254 kb on - strand, within Pf6N2E2_4686 at 5421.385 kb on - strand at 5421.415 kb on + strand at 5421.416 kb on - strand at 5421.479 kb on + strand at 5421.480 kb on - strand at 5421.488 kb on + strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.642 kb on + strand, within Pf6N2E2_4687 at 5421.643 kb on - strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.757 kb on + strand, within Pf6N2E2_4687 at 5421.818 kb on + strand at 5421.818 kb on + strand at 5421.819 kb on - strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.923 kb on - strand at 5421.923 kb on - strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.283 kb on - strand, within Pf6N2E2_4688
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 5,419,317 + Pf6N2E2_4685 0.69 -1.2 5,419,318 - Pf6N2E2_4685 0.69 +1.0 5,419,318 - Pf6N2E2_4685 0.69 +1.0 5,419,689 + -1.6 5,419,689 + +0.0 5,419,689 + +0.0 5,419,689 + -0.6 5,419,689 + +2.0 5,419,690 - -0.6 5,419,690 - +1.0 5,419,691 + -0.6 5,419,691 + -0.4 5,419,691 + -0.5 5,419,691 + +1.0 5,419,691 + +1.0 5,419,692 - +1.0 5,419,887 - +0.0 5,419,887 - +0.0 5,419,898 + +0.0 5,419,899 - +1.0 5,419,899 - -0.6 5,419,933 + -1.0 5,419,934 - +0.6 5,420,017 + +1.0 5,420,017 + +1.0 5,420,017 + -0.5 5,420,018 - +0.0 5,420,018 - +0.0 5,420,059 - +1.0 5,420,130 + +1.0 5,420,130 + -1.2 5,420,131 - +1.0 5,420,131 - +1.0 5,420,131 - -0.8 5,420,131 - -0.6 5,420,131 - -0.6 5,420,141 + +1.0 5,420,142 - +0.3 5,420,171 + +0.0 5,420,172 - -1.0 5,420,172 - +0.0 5,420,172 - +0.0 5,420,217 + -1.0 5,420,217 + -0.8 5,420,217 + +1.0 5,420,218 - -1.0 5,420,218 - +1.0 5,420,218 - +0.0 5,420,218 - +0.0 5,420,252 + -0.9 5,420,307 + -0.5 5,420,307 + -0.6 5,420,417 + -0.5 5,420,426 + Pf6N2E2_4686 0.10 -0.4 5,420,459 + Pf6N2E2_4686 0.13 +0.2 5,420,459 + Pf6N2E2_4686 0.13 +0.2 5,420,459 + Pf6N2E2_4686 0.13 +0.3 5,420,459 + Pf6N2E2_4686 0.13 +1.0 5,420,460 - Pf6N2E2_4686 0.13 -1.0 5,420,460 - Pf6N2E2_4686 0.13 +1.0 5,420,460 - Pf6N2E2_4686 0.13 +0.2 5,420,460 - Pf6N2E2_4686 0.13 -0.6 5,420,461 + Pf6N2E2_4686 0.13 +0.0 5,420,462 - Pf6N2E2_4686 0.13 +1.0 5,420,562 + Pf6N2E2_4686 0.22 +1.0 5,420,599 - Pf6N2E2_4686 0.26 +0.0 5,420,781 + Pf6N2E2_4686 0.42 +1.0 5,420,915 - Pf6N2E2_4686 0.54 +0.1 5,421,058 + Pf6N2E2_4686 0.67 +0.0 5,421,058 + Pf6N2E2_4686 0.67 +1.0 5,421,058 + Pf6N2E2_4686 0.67 +1.0 5,421,058 + Pf6N2E2_4686 0.67 +0.0 5,421,059 - Pf6N2E2_4686 0.67 +0.5 5,421,104 - Pf6N2E2_4686 0.72 +0.0 5,421,104 - Pf6N2E2_4686 0.72 +1.0 5,421,127 + Pf6N2E2_4686 0.74 +1.9 5,421,127 + Pf6N2E2_4686 0.74 -0.6 5,421,128 - Pf6N2E2_4686 0.74 -1.0 5,421,130 + Pf6N2E2_4686 0.74 +1.0 5,421,130 + Pf6N2E2_4686 0.74 +0.0 5,421,130 + Pf6N2E2_4686 0.74 +1.0 5,421,130 + Pf6N2E2_4686 0.74 +1.0 5,421,130 + Pf6N2E2_4686 0.74 +1.0 5,421,130 + Pf6N2E2_4686 0.74 +1.0 5,421,131 - Pf6N2E2_4686 0.74 +0.2 5,421,131 - Pf6N2E2_4686 0.74 +0.1 5,421,207 - Pf6N2E2_4686 0.81 +0.0 5,421,254 - Pf6N2E2_4686 0.85 -0.5 5,421,385 - -2.1 5,421,415 + +0.6 5,421,416 - +1.0 5,421,479 + +1.0 5,421,480 - +1.0 5,421,488 + -0.3 5,421,489 - -0.6 5,421,489 - +0.0 5,421,489 - +0.0 5,421,489 - +1.0 5,421,613 - Pf6N2E2_4687 0.19 +1.0 5,421,613 - Pf6N2E2_4687 0.19 +0.0 5,421,613 - Pf6N2E2_4687 0.19 +1.0 5,421,613 - Pf6N2E2_4687 0.19 +0.0 5,421,613 - Pf6N2E2_4687 0.19 -0.6 5,421,642 + Pf6N2E2_4687 0.30 +1.0 5,421,643 - Pf6N2E2_4687 0.31 +0.0 5,421,659 + Pf6N2E2_4687 0.37 +1.0 5,421,659 + Pf6N2E2_4687 0.37 +1.0 5,421,659 + Pf6N2E2_4687 0.37 +1.0 5,421,659 + Pf6N2E2_4687 0.37 -0.1 5,421,659 + Pf6N2E2_4687 0.37 +1.0 5,421,660 - Pf6N2E2_4687 0.37 +1.0 5,421,660 - Pf6N2E2_4687 0.37 -0.9 5,421,660 - Pf6N2E2_4687 0.37 +0.0 5,421,660 - Pf6N2E2_4687 0.37 -0.6 5,421,660 - Pf6N2E2_4687 0.37 -0.8 5,421,660 - Pf6N2E2_4687 0.37 +0.0 5,421,660 - Pf6N2E2_4687 0.37 +1.0 5,421,757 + Pf6N2E2_4687 0.74 +0.6 5,421,818 + +1.0 5,421,818 + +1.0 5,421,819 - +0.0 5,421,922 + +1.0 5,421,922 + +1.0 5,421,922 + -0.2 5,421,923 - -0.2 5,421,923 - -0.4 5,422,175 + -0.6 5,422,175 + +0.0 5,422,175 + -0.7 5,422,175 + +1.0 5,422,175 + +0.4 5,422,175 + -1.0 5,422,175 + +1.0 5,422,175 + -0.2 5,422,175 + -0.6 5,422,175 + +0.0 5,422,175 + -0.5 5,422,176 - -1.6 5,422,176 - +1.0 5,422,176 - -0.5 5,422,176 - +0.0 5,422,176 - -0.6 5,422,176 - +0.0 5,422,176 - +1.0 5,422,176 - +0.2 5,422,176 - +0.0 5,422,176 - +0.1 5,422,176 - +1.0 5,422,176 - -0.9 5,422,176 - -0.5 5,422,283 - Pf6N2E2_4688 0.13 +1.0
Or see this region's nucleotide sequence