Experiment: Ying_AminoAcid20 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3712 and Pf6N2E2_3713 are separated by 9 nucleotides Pf6N2E2_3713 and Pf6N2E2_3714 overlap by 4 nucleotides Pf6N2E2_3714 and Pf6N2E2_3715 overlap by 4 nucleotides Pf6N2E2_3715 and Pf6N2E2_3716 are separated by 146 nucleotides Pf6N2E2_3716 and Pf6N2E2_3717 overlap by 4 nucleotides
Pf6N2E2_3712 - Acyl carrier protein (ACP1), at 4,326,719 to 4,326,979
_3712
Pf6N2E2_3713 - Acyl carrier protein, at 4,326,989 to 4,327,243
_3713
Pf6N2E2_3714 - FIG017861: hypothetical protein, at 4,327,240 to 4,327,785
_3714
Pf6N2E2_3715 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 4,327,782 to 4,329,461
_3715
Pf6N2E2_3716 - FIG143263: Glycosyl transferase, at 4,329,608 to 4,330,342
_3716
Pf6N2E2_3717 - Lysophospholipid acyltransferase, at 4,330,339 to 4,331,283
_3717
Position (kb) 4327
4328
4329
4330 Strain fitness (log2 ratio) -1
0
1
2 at 4326.858 kb on + strand, within Pf6N2E2_3712 at 4326.882 kb on - strand, within Pf6N2E2_3712 at 4326.894 kb on + strand, within Pf6N2E2_3712 at 4327.007 kb on - strand at 4327.086 kb on - strand, within Pf6N2E2_3713 at 4327.135 kb on + strand, within Pf6N2E2_3713 at 4327.135 kb on + strand, within Pf6N2E2_3713 at 4327.136 kb on - strand, within Pf6N2E2_3713 at 4327.300 kb on + strand, within Pf6N2E2_3714 at 4327.528 kb on + strand, within Pf6N2E2_3714 at 4327.597 kb on + strand, within Pf6N2E2_3714 at 4327.597 kb on + strand, within Pf6N2E2_3714 at 4327.597 kb on + strand, within Pf6N2E2_3714 at 4327.598 kb on - strand, within Pf6N2E2_3714 at 4327.598 kb on - strand, within Pf6N2E2_3714 at 4327.598 kb on - strand, within Pf6N2E2_3714 at 4327.599 kb on + strand, within Pf6N2E2_3714 at 4327.599 kb on + strand, within Pf6N2E2_3714 at 4327.600 kb on - strand, within Pf6N2E2_3714 at 4327.641 kb on + strand, within Pf6N2E2_3714 at 4327.700 kb on - strand, within Pf6N2E2_3714 at 4327.700 kb on - strand, within Pf6N2E2_3714 at 4327.770 kb on - strand at 4328.215 kb on + strand, within Pf6N2E2_3715 at 4328.215 kb on + strand, within Pf6N2E2_3715 at 4328.216 kb on - strand, within Pf6N2E2_3715 at 4328.216 kb on - strand, within Pf6N2E2_3715 at 4328.304 kb on + strand, within Pf6N2E2_3715 at 4328.305 kb on - strand, within Pf6N2E2_3715 at 4328.305 kb on - strand, within Pf6N2E2_3715 at 4328.695 kb on - strand, within Pf6N2E2_3715 at 4328.764 kb on + strand, within Pf6N2E2_3715 at 4328.794 kb on + strand, within Pf6N2E2_3715 at 4328.794 kb on + strand, within Pf6N2E2_3715 at 4328.795 kb on - strand, within Pf6N2E2_3715 at 4328.795 kb on - strand, within Pf6N2E2_3715 at 4328.795 kb on - strand, within Pf6N2E2_3715 at 4329.390 kb on + strand at 4329.391 kb on - strand at 4329.391 kb on - strand at 4329.439 kb on + strand at 4329.439 kb on + strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.440 kb on - strand at 4329.459 kb on + strand at 4329.460 kb on - strand at 4329.528 kb on + strand at 4329.528 kb on + strand at 4329.529 kb on - strand at 4329.639 kb on - strand at 4329.639 kb on - strand at 4329.966 kb on - strand, within Pf6N2E2_3716 at 4329.996 kb on - strand, within Pf6N2E2_3716 at 4329.996 kb on - strand, within Pf6N2E2_3716 at 4330.038 kb on + strand, within Pf6N2E2_3716 at 4330.038 kb on + strand, within Pf6N2E2_3716 at 4330.039 kb on - strand, within Pf6N2E2_3716 at 4330.434 kb on - strand, within Pf6N2E2_3717
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 2 remove 4,326,858 + Pf6N2E2_3712 0.53 +0.1 4,326,882 - Pf6N2E2_3712 0.62 -0.9 4,326,894 + Pf6N2E2_3712 0.67 +1.0 4,327,007 - +1.6 4,327,086 - Pf6N2E2_3713 0.38 +1.0 4,327,135 + Pf6N2E2_3713 0.57 +0.4 4,327,135 + Pf6N2E2_3713 0.57 +1.6 4,327,136 - Pf6N2E2_3713 0.58 +0.0 4,327,300 + Pf6N2E2_3714 0.11 +1.0 4,327,528 + Pf6N2E2_3714 0.53 -0.3 4,327,597 + Pf6N2E2_3714 0.65 -0.3 4,327,597 + Pf6N2E2_3714 0.65 +1.0 4,327,597 + Pf6N2E2_3714 0.65 +1.0 4,327,598 - Pf6N2E2_3714 0.66 +0.0 4,327,598 - Pf6N2E2_3714 0.66 +1.0 4,327,598 - Pf6N2E2_3714 0.66 -0.1 4,327,599 + Pf6N2E2_3714 0.66 -1.0 4,327,599 + Pf6N2E2_3714 0.66 -0.6 4,327,600 - Pf6N2E2_3714 0.66 +1.0 4,327,641 + Pf6N2E2_3714 0.73 +0.0 4,327,700 - Pf6N2E2_3714 0.84 -0.6 4,327,700 - Pf6N2E2_3714 0.84 -0.4 4,327,770 - +1.0 4,328,215 + Pf6N2E2_3715 0.26 +0.4 4,328,215 + Pf6N2E2_3715 0.26 -0.3 4,328,216 - Pf6N2E2_3715 0.26 +0.0 4,328,216 - Pf6N2E2_3715 0.26 +1.0 4,328,304 + Pf6N2E2_3715 0.31 +0.0 4,328,305 - Pf6N2E2_3715 0.31 +0.4 4,328,305 - Pf6N2E2_3715 0.31 +2.0 4,328,695 - Pf6N2E2_3715 0.54 +0.0 4,328,764 + Pf6N2E2_3715 0.58 +0.0 4,328,794 + Pf6N2E2_3715 0.60 +0.4 4,328,794 + Pf6N2E2_3715 0.60 +0.6 4,328,795 - Pf6N2E2_3715 0.60 +0.0 4,328,795 - Pf6N2E2_3715 0.60 -0.6 4,328,795 - Pf6N2E2_3715 0.60 +1.0 4,329,390 + +1.0 4,329,391 - +1.0 4,329,391 - +1.0 4,329,439 + -0.5 4,329,439 + +0.0 4,329,440 - -0.6 4,329,440 - +0.0 4,329,440 - +1.0 4,329,440 - +1.0 4,329,459 + -0.4 4,329,460 - +1.0 4,329,528 + +1.0 4,329,528 + +1.0 4,329,529 - +1.0 4,329,639 - -0.7 4,329,639 - +0.0 4,329,966 - Pf6N2E2_3716 0.49 +1.0 4,329,996 - Pf6N2E2_3716 0.53 +1.0 4,329,996 - Pf6N2E2_3716 0.53 +1.0 4,330,038 + Pf6N2E2_3716 0.59 -0.4 4,330,038 + Pf6N2E2_3716 0.59 -0.3 4,330,039 - Pf6N2E2_3716 0.59 +0.0 4,330,434 - Pf6N2E2_3717 0.10 +0.0
Or see this region's nucleotide sequence