Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_799

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_797 and Pf6N2E2_798 are separated by 32 nucleotidesPf6N2E2_798 and Pf6N2E2_799 overlap by 48 nucleotidesPf6N2E2_799 and Pf6N2E2_800 are separated by 49 nucleotidesPf6N2E2_800 and Pf6N2E2_801 are separated by 47 nucleotides Pf6N2E2_797 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 928,292 to 930,259 _797 Pf6N2E2_798 - Ku domain protein, at 930,292 to 931,182 _798 Pf6N2E2_799 - hypothetical protein, at 931,135 to 931,344 _799 Pf6N2E2_800 - FIG00962948: hypothetical protein, at 931,394 to 931,723 _800 Pf6N2E2_801 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 931,771 to 932,919 _801 Position (kb) 931 932Strain fitness (log2 ratio) -2 -1 0 1 2at 930.274 kb on + strandat 930.274 kb on + strandat 930.275 kb on - strandat 930.673 kb on + strand, within Pf6N2E2_798at 930.673 kb on + strand, within Pf6N2E2_798at 930.673 kb on + strand, within Pf6N2E2_798at 930.674 kb on - strand, within Pf6N2E2_798at 930.674 kb on - strand, within Pf6N2E2_798at 930.815 kb on + strand, within Pf6N2E2_798at 930.816 kb on - strand, within Pf6N2E2_798at 930.816 kb on - strand, within Pf6N2E2_798at 930.816 kb on - strand, within Pf6N2E2_798at 930.816 kb on - strand, within Pf6N2E2_798at 930.968 kb on + strand, within Pf6N2E2_798at 930.968 kb on + strand, within Pf6N2E2_798at 931.295 kb on + strand, within Pf6N2E2_799at 931.295 kb on + strand, within Pf6N2E2_799at 931.320 kb on + strand, within Pf6N2E2_799at 931.414 kb on - strandat 931.414 kb on - strandat 931.414 kb on - strandat 931.416 kb on + strandat 931.416 kb on + strandat 931.416 kb on + strandat 931.417 kb on - strandat 931.417 kb on - strandat 931.499 kb on + strand, within Pf6N2E2_800at 931.499 kb on + strand, within Pf6N2E2_800at 931.499 kb on + strand, within Pf6N2E2_800at 931.500 kb on - strand, within Pf6N2E2_800at 931.500 kb on - strand, within Pf6N2E2_800at 931.501 kb on + strand, within Pf6N2E2_800at 931.502 kb on - strand, within Pf6N2E2_800at 931.502 kb on - strand, within Pf6N2E2_800at 931.566 kb on + strand, within Pf6N2E2_800at 931.566 kb on + strand, within Pf6N2E2_800at 931.566 kb on + strand, within Pf6N2E2_800at 931.566 kb on + strand, within Pf6N2E2_800at 931.566 kb on + strand, within Pf6N2E2_800at 931.567 kb on - strand, within Pf6N2E2_800at 931.567 kb on - strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.570 kb on + strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.571 kb on - strand, within Pf6N2E2_800at 931.779 kb on + strandat 931.779 kb on + strandat 931.780 kb on - strandat 931.780 kb on - strandat 931.780 kb on - strandat 932.107 kb on + strand, within Pf6N2E2_801at 932.108 kb on - strand, within Pf6N2E2_801at 932.108 kb on - strand, within Pf6N2E2_801at 932.108 kb on - strand, within Pf6N2E2_801at 932.117 kb on - strand, within Pf6N2E2_801at 932.117 kb on - strand, within Pf6N2E2_801at 932.281 kb on + strand, within Pf6N2E2_801at 932.282 kb on - strand, within Pf6N2E2_801at 932.292 kb on + strand, within Pf6N2E2_801at 932.292 kb on + strand, within Pf6N2E2_801at 932.293 kb on - strand, within Pf6N2E2_801at 932.293 kb on - strand, within Pf6N2E2_801at 932.293 kb on - strand, within Pf6N2E2_801

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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930,274 + -0.6
930,274 + -0.1
930,275 - +0.9
930,673 + Pf6N2E2_798 0.43 +0.9
930,673 + Pf6N2E2_798 0.43 -0.1
930,673 + Pf6N2E2_798 0.43 +0.9
930,674 - Pf6N2E2_798 0.43 +0.9
930,674 - Pf6N2E2_798 0.43 +0.9
930,815 + Pf6N2E2_798 0.59 -0.6
930,816 - Pf6N2E2_798 0.59 -0.1
930,816 - Pf6N2E2_798 0.59 +0.9
930,816 - Pf6N2E2_798 0.59 -0.1
930,816 - Pf6N2E2_798 0.59 -0.6
930,968 + Pf6N2E2_798 0.76 +0.9
930,968 + Pf6N2E2_798 0.76 +0.9
931,295 + Pf6N2E2_799 0.76 +0.9
931,295 + Pf6N2E2_799 0.76 +0.9
931,320 + Pf6N2E2_799 0.88 -0.1
931,414 - -0.5
931,414 - +0.9
931,414 - -0.7
931,416 + -0.1
931,416 + +0.9
931,416 + -1.3
931,417 - +0.9
931,417 - +0.9
931,499 + Pf6N2E2_800 0.32 +0.9
931,499 + Pf6N2E2_800 0.32 +0.9
931,499 + Pf6N2E2_800 0.32 +0.9
931,500 - Pf6N2E2_800 0.32 +0.9
931,500 - Pf6N2E2_800 0.32 +0.9
931,501 + Pf6N2E2_800 0.32 -1.0
931,502 - Pf6N2E2_800 0.33 -0.8
931,502 - Pf6N2E2_800 0.33 -0.4
931,566 + Pf6N2E2_800 0.52 -0.6
931,566 + Pf6N2E2_800 0.52 -0.7
931,566 + Pf6N2E2_800 0.52 +0.9
931,566 + Pf6N2E2_800 0.52 -0.1
931,566 + Pf6N2E2_800 0.52 +0.9
931,567 - Pf6N2E2_800 0.52 +0.9
931,567 - Pf6N2E2_800 0.52 -0.5
931,570 + Pf6N2E2_800 0.53 +0.9
931,570 + Pf6N2E2_800 0.53 +0.9
931,570 + Pf6N2E2_800 0.53 -0.5
931,570 + Pf6N2E2_800 0.53 -0.4
931,570 + Pf6N2E2_800 0.53 +0.9
931,570 + Pf6N2E2_800 0.53 -0.1
931,570 + Pf6N2E2_800 0.53 -0.1
931,571 - Pf6N2E2_800 0.54 -1.7
931,571 - Pf6N2E2_800 0.54 -2.1
931,571 - Pf6N2E2_800 0.54 +0.9
931,571 - Pf6N2E2_800 0.54 -0.1
931,571 - Pf6N2E2_800 0.54 +0.9
931,571 - Pf6N2E2_800 0.54 -0.5
931,571 - Pf6N2E2_800 0.54 -0.1
931,779 + -0.1
931,779 + -0.1
931,780 - +1.9
931,780 - +0.9
931,780 - +0.9
932,107 + Pf6N2E2_801 0.29 -0.9
932,108 - Pf6N2E2_801 0.29 +0.9
932,108 - Pf6N2E2_801 0.29 -0.1
932,108 - Pf6N2E2_801 0.29 -0.7
932,117 - Pf6N2E2_801 0.30 -0.1
932,117 - Pf6N2E2_801 0.30 -1.0
932,281 + Pf6N2E2_801 0.44 +0.9
932,282 - Pf6N2E2_801 0.44 -0.7
932,292 + Pf6N2E2_801 0.45 -0.1
932,292 + Pf6N2E2_801 0.45 -1.4
932,293 - Pf6N2E2_801 0.45 -0.3
932,293 - Pf6N2E2_801 0.45 +0.9
932,293 - Pf6N2E2_801 0.45 +0.9

Or see this region's nucleotide sequence