Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4509 and Pf6N2E2_4510 are separated by 383 nucleotides Pf6N2E2_4510 and Pf6N2E2_4511 are separated by 40 nucleotides
Pf6N2E2_4509 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) <small>(from data)</small>, at 5,240,232 to 5,241,503
_4509
Pf6N2E2_4510 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) <small>(from data)</small>, at 5,241,887 to 5,242,663
_4510
Pf6N2E2_4511 - glutamine synthetase family protein, at 5,242,704 to 5,244,062
_4511
Position (kb) 5241
5242
5243 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5240.911 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.049 kb on + strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.050 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.074 kb on - strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.160 kb on + strand, within Pf6N2E2_4509 at 5241.161 kb on - strand, within Pf6N2E2_4509 at 5241.402 kb on - strand at 5241.426 kb on + strand at 5241.426 kb on + strand at 5241.427 kb on - strand at 5241.457 kb on + strand at 5241.483 kb on + strand at 5241.484 kb on - strand at 5241.484 kb on - strand at 5241.512 kb on + strand at 5241.535 kb on + strand at 5241.536 kb on - strand at 5241.536 kb on - strand at 5241.579 kb on - strand at 5241.628 kb on + strand at 5241.634 kb on + strand at 5241.803 kb on - strand at 5241.825 kb on - strand at 5241.842 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.870 kb on + strand at 5241.871 kb on - strand at 5241.873 kb on + strand at 5241.947 kb on + strand at 5241.947 kb on + strand at 5241.968 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.098 kb on + strand, within Pf6N2E2_4510 at 5242.099 kb on - strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.248 kb on + strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.249 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.319 kb on - strand, within Pf6N2E2_4510 at 5242.340 kb on + strand, within Pf6N2E2_4510 at 5242.364 kb on - strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.380 kb on + strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.381 kb on - strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.410 kb on + strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510 at 5242.411 kb on - strand, within Pf6N2E2_4510 at 5242.533 kb on + strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.534 kb on - strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.563 kb on + strand, within Pf6N2E2_4510 at 5242.564 kb on - strand, within Pf6N2E2_4510 at 5242.571 kb on - strand, within Pf6N2E2_4510 at 5242.580 kb on + strand, within Pf6N2E2_4510 at 5242.580 kb on + strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.581 kb on - strand, within Pf6N2E2_4510 at 5242.668 kb on + strand at 5242.668 kb on + strand at 5242.669 kb on - strand at 5242.669 kb on - strand at 5242.671 kb on + strand at 5242.672 kb on - strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.703 kb on + strand at 5242.704 kb on - strand at 5242.704 kb on - strand at 5242.710 kb on - strand at 5242.887 kb on + strand, within Pf6N2E2_4511 at 5242.887 kb on + strand, within Pf6N2E2_4511 at 5242.888 kb on - strand, within Pf6N2E2_4511 at 5242.888 kb on - strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.905 kb on + strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.906 kb on - strand, within Pf6N2E2_4511 at 5242.909 kb on - strand, within Pf6N2E2_4511 at 5242.909 kb on - strand, within Pf6N2E2_4511 at 5242.966 kb on + strand, within Pf6N2E2_4511 at 5243.019 kb on + strand, within Pf6N2E2_4511 at 5243.020 kb on - strand, within Pf6N2E2_4511 at 5243.074 kb on + strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.075 kb on - strand, within Pf6N2E2_4511 at 5243.085 kb on + strand, within Pf6N2E2_4511 at 5243.086 kb on - strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.133 kb on + strand, within Pf6N2E2_4511 at 5243.134 kb on - strand, within Pf6N2E2_4511 at 5243.269 kb on - strand, within Pf6N2E2_4511 at 5243.431 kb on + strand, within Pf6N2E2_4511 at 5243.431 kb on + strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.432 kb on - strand, within Pf6N2E2_4511 at 5243.606 kb on - strand, within Pf6N2E2_4511
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 5,240,911 + Pf6N2E2_4509 0.53 +1.0 5,240,911 + Pf6N2E2_4509 0.53 +0.0 5,240,911 + Pf6N2E2_4509 0.53 -1.6 5,241,049 + Pf6N2E2_4509 0.64 +0.0 5,241,049 + Pf6N2E2_4509 0.64 +0.0 5,241,050 - Pf6N2E2_4509 0.64 -0.6 5,241,050 - Pf6N2E2_4509 0.64 -0.7 5,241,074 - Pf6N2E2_4509 0.66 +0.0 5,241,074 - Pf6N2E2_4509 0.66 -1.7 5,241,160 + Pf6N2E2_4509 0.73 +0.0 5,241,160 + Pf6N2E2_4509 0.73 -0.5 5,241,160 + Pf6N2E2_4509 0.73 +1.0 5,241,161 - Pf6N2E2_4509 0.73 +0.0 5,241,402 - +1.0 5,241,426 + -0.6 5,241,426 + +0.0 5,241,427 - +0.0 5,241,457 + -0.4 5,241,483 + +0.0 5,241,484 - +0.0 5,241,484 - -0.4 5,241,512 + +0.0 5,241,535 + -0.4 5,241,536 - +0.0 5,241,536 - -0.5 5,241,579 - -1.6 5,241,628 + +2.6 5,241,634 + -0.6 5,241,803 - +1.0 5,241,825 - -0.6 5,241,842 + -0.6 5,241,870 + -1.0 5,241,870 + +0.0 5,241,870 + +0.0 5,241,871 - +1.0 5,241,873 + +1.0 5,241,947 + +1.0 5,241,947 + +1.0 5,241,968 + Pf6N2E2_4510 0.10 +0.4 5,242,098 + Pf6N2E2_4510 0.27 +0.0 5,242,098 + Pf6N2E2_4510 0.27 +0.0 5,242,098 + Pf6N2E2_4510 0.27 +1.0 5,242,098 + Pf6N2E2_4510 0.27 -0.6 5,242,098 + Pf6N2E2_4510 0.27 -0.6 5,242,099 - Pf6N2E2_4510 0.27 +1.0 5,242,248 + Pf6N2E2_4510 0.46 -0.6 5,242,248 + Pf6N2E2_4510 0.46 -0.3 5,242,249 - Pf6N2E2_4510 0.47 -0.6 5,242,249 - Pf6N2E2_4510 0.47 +0.0 5,242,319 - Pf6N2E2_4510 0.56 -1.8 5,242,319 - Pf6N2E2_4510 0.56 +1.0 5,242,340 + Pf6N2E2_4510 0.58 +1.0 5,242,364 - Pf6N2E2_4510 0.61 +0.0 5,242,380 + Pf6N2E2_4510 0.63 +1.0 5,242,380 + Pf6N2E2_4510 0.63 +1.0 5,242,381 - Pf6N2E2_4510 0.64 +1.0 5,242,381 - Pf6N2E2_4510 0.64 +0.0 5,242,381 - Pf6N2E2_4510 0.64 +1.0 5,242,381 - Pf6N2E2_4510 0.64 +0.0 5,242,381 - Pf6N2E2_4510 0.64 -1.0 5,242,410 + Pf6N2E2_4510 0.67 -0.6 5,242,410 + Pf6N2E2_4510 0.67 +0.0 5,242,410 + Pf6N2E2_4510 0.67 +0.0 5,242,410 + Pf6N2E2_4510 0.67 +1.0 5,242,411 - Pf6N2E2_4510 0.67 -1.0 5,242,411 - Pf6N2E2_4510 0.67 +1.0 5,242,533 + Pf6N2E2_4510 0.83 +1.0 5,242,534 - Pf6N2E2_4510 0.83 -0.6 5,242,534 - Pf6N2E2_4510 0.83 +0.0 5,242,534 - Pf6N2E2_4510 0.83 +1.0 5,242,563 + Pf6N2E2_4510 0.87 +0.0 5,242,563 + Pf6N2E2_4510 0.87 +0.0 5,242,563 + Pf6N2E2_4510 0.87 -0.6 5,242,564 - Pf6N2E2_4510 0.87 +1.0 5,242,571 - Pf6N2E2_4510 0.88 +0.0 5,242,580 + Pf6N2E2_4510 0.89 +1.0 5,242,580 + Pf6N2E2_4510 0.89 +1.0 5,242,581 - Pf6N2E2_4510 0.89 +0.0 5,242,581 - Pf6N2E2_4510 0.89 -0.6 5,242,581 - Pf6N2E2_4510 0.89 +0.3 5,242,581 - Pf6N2E2_4510 0.89 +1.0 5,242,581 - Pf6N2E2_4510 0.89 -1.3 5,242,668 + -1.8 5,242,668 + +1.0 5,242,669 - +1.0 5,242,669 - +1.0 5,242,671 + +0.0 5,242,672 - +1.0 5,242,703 + +0.0 5,242,703 + +0.0 5,242,703 + +0.0 5,242,703 + +1.0 5,242,703 + +0.0 5,242,703 + +0.0 5,242,704 - -1.3 5,242,704 - +1.0 5,242,710 - -0.6 5,242,887 + Pf6N2E2_4511 0.13 +1.0 5,242,887 + Pf6N2E2_4511 0.13 -0.7 5,242,888 - Pf6N2E2_4511 0.14 +1.0 5,242,888 - Pf6N2E2_4511 0.14 -0.5 5,242,905 + Pf6N2E2_4511 0.15 -1.0 5,242,905 + Pf6N2E2_4511 0.15 +0.0 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,905 + Pf6N2E2_4511 0.15 -0.3 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,905 + Pf6N2E2_4511 0.15 +0.0 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,905 + Pf6N2E2_4511 0.15 +1.0 5,242,906 - Pf6N2E2_4511 0.15 -0.8 5,242,906 - Pf6N2E2_4511 0.15 +0.0 5,242,906 - Pf6N2E2_4511 0.15 +0.0 5,242,906 - Pf6N2E2_4511 0.15 +0.0 5,242,906 - Pf6N2E2_4511 0.15 +1.0 5,242,906 - Pf6N2E2_4511 0.15 -0.4 5,242,906 - Pf6N2E2_4511 0.15 +1.0 5,242,906 - Pf6N2E2_4511 0.15 +0.0 5,242,906 - Pf6N2E2_4511 0.15 +0.0 5,242,909 - Pf6N2E2_4511 0.15 +1.0 5,242,909 - Pf6N2E2_4511 0.15 -1.4 5,242,966 + Pf6N2E2_4511 0.19 +1.0 5,243,019 + Pf6N2E2_4511 0.23 -0.6 5,243,020 - Pf6N2E2_4511 0.23 +1.0 5,243,074 + Pf6N2E2_4511 0.27 -1.0 5,243,075 - Pf6N2E2_4511 0.27 +0.0 5,243,075 - Pf6N2E2_4511 0.27 +0.0 5,243,075 - Pf6N2E2_4511 0.27 +0.0 5,243,075 - Pf6N2E2_4511 0.27 +1.0 5,243,085 + Pf6N2E2_4511 0.28 +0.0 5,243,086 - Pf6N2E2_4511 0.28 +0.0 5,243,133 + Pf6N2E2_4511 0.32 +0.0 5,243,133 + Pf6N2E2_4511 0.32 +0.0 5,243,133 + Pf6N2E2_4511 0.32 +0.6 5,243,133 + Pf6N2E2_4511 0.32 +1.0 5,243,133 + Pf6N2E2_4511 0.32 +0.0 5,243,134 - Pf6N2E2_4511 0.32 +1.0 5,243,269 - Pf6N2E2_4511 0.42 -1.3 5,243,431 + Pf6N2E2_4511 0.53 +1.0 5,243,431 + Pf6N2E2_4511 0.53 +1.0 5,243,432 - Pf6N2E2_4511 0.54 -0.3 5,243,432 - Pf6N2E2_4511 0.54 -1.0 5,243,432 - Pf6N2E2_4511 0.54 -1.0 5,243,432 - Pf6N2E2_4511 0.54 -1.0 5,243,432 - Pf6N2E2_4511 0.54 -1.8 5,243,432 - Pf6N2E2_4511 0.54 +0.0 5,243,606 - Pf6N2E2_4511 0.66 -0.6
Or see this region's nucleotide sequence