Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4494

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4492 and Pf6N2E2_4493 are separated by 126 nucleotidesPf6N2E2_4493 and Pf6N2E2_4494 are separated by 123 nucleotidesPf6N2E2_4494 and Pf6N2E2_4495 are separated by 24 nucleotidesPf6N2E2_4495 and Pf6N2E2_4496 are separated by 14 nucleotides Pf6N2E2_4492 - FIG00953395: hypothetical protein, at 5,225,247 to 5,225,585 _4492 Pf6N2E2_4493 - Transcriptional regulator, AsnC family, at 5,225,712 to 5,226,182 _4493 Pf6N2E2_4494 - Adenylate cyclase (EC 4.6.1.1), at 5,226,306 to 5,227,673 _4494 Pf6N2E2_4495 - hypothetical protein, at 5,227,698 to 5,227,817 _4495 Pf6N2E2_4496 - Acetylornithine deacetylase (EC 3.5.1.16), at 5,227,832 to 5,228,989 _4496 Position (kb) 5226 5227 5228Strain fitness (log2 ratio) -2 -1 0 1 2at 5225.324 kb on + strand, within Pf6N2E2_4492at 5225.325 kb on - strand, within Pf6N2E2_4492at 5225.325 kb on - strand, within Pf6N2E2_4492at 5225.328 kb on + strand, within Pf6N2E2_4492at 5225.329 kb on - strand, within Pf6N2E2_4492at 5225.472 kb on + strand, within Pf6N2E2_4492at 5225.472 kb on + strand, within Pf6N2E2_4492at 5225.473 kb on - strand, within Pf6N2E2_4492at 5225.503 kb on + strand, within Pf6N2E2_4492at 5225.559 kb on + strandat 5225.634 kb on - strandat 5225.645 kb on - strandat 5225.645 kb on - strandat 5225.687 kb on + strandat 5225.906 kb on + strand, within Pf6N2E2_4493at 5225.906 kb on + strand, within Pf6N2E2_4493at 5225.906 kb on + strand, within Pf6N2E2_4493at 5225.906 kb on + strand, within Pf6N2E2_4493at 5225.907 kb on - strand, within Pf6N2E2_4493at 5225.998 kb on + strand, within Pf6N2E2_4493at 5225.998 kb on + strand, within Pf6N2E2_4493at 5225.999 kb on - strand, within Pf6N2E2_4493at 5225.999 kb on - strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.009 kb on + strand, within Pf6N2E2_4493at 5226.010 kb on - strand, within Pf6N2E2_4493at 5226.010 kb on - strand, within Pf6N2E2_4493at 5226.010 kb on - strand, within Pf6N2E2_4493at 5226.010 kb on - strand, within Pf6N2E2_4493at 5226.069 kb on + strand, within Pf6N2E2_4493at 5226.069 kb on + strand, within Pf6N2E2_4493at 5226.070 kb on - strand, within Pf6N2E2_4493at 5226.070 kb on - strand, within Pf6N2E2_4493at 5226.070 kb on - strand, within Pf6N2E2_4493at 5226.070 kb on - strand, within Pf6N2E2_4493at 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.249 kb on + strandat 5226.250 kb on - strandat 5226.250 kb on - strandat 5226.250 kb on - strandat 5226.250 kb on - strandat 5226.259 kb on + strandat 5226.343 kb on + strandat 5226.343 kb on + strandat 5226.343 kb on + strandat 5226.433 kb on + strandat 5227.049 kb on - strand, within Pf6N2E2_4494at 5227.049 kb on - strand, within Pf6N2E2_4494at 5227.064 kb on - strand, within Pf6N2E2_4494at 5227.417 kb on + strand, within Pf6N2E2_4494at 5227.417 kb on + strand, within Pf6N2E2_4494at 5227.418 kb on - strand, within Pf6N2E2_4494at 5227.549 kb on + strandat 5227.549 kb on + strandat 5227.550 kb on - strandat 5227.550 kb on - strandat 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.739 kb on + strand, within Pf6N2E2_4495at 5227.758 kb on - strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strand, within Pf6N2E2_4495at 5227.796 kb on + strandat 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.797 kb on - strand, within Pf6N2E2_4495at 5227.815 kb on + strandat 5227.815 kb on + strandat 5227.815 kb on + strandat 5227.816 kb on - strandat 5227.816 kb on - strandat 5228.046 kb on + strand, within Pf6N2E2_4496

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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5,225,324 + Pf6N2E2_4492 0.23 +0.0
5,225,325 - Pf6N2E2_4492 0.23 -0.4
5,225,325 - Pf6N2E2_4492 0.23 +0.5
5,225,328 + Pf6N2E2_4492 0.24 +1.0
5,225,329 - Pf6N2E2_4492 0.24 +1.0
5,225,472 + Pf6N2E2_4492 0.66 -0.3
5,225,472 + Pf6N2E2_4492 0.66 +1.3
5,225,473 - Pf6N2E2_4492 0.67 -1.4
5,225,503 + Pf6N2E2_4492 0.76 +1.0
5,225,559 + +0.0
5,225,634 - +1.4
5,225,645 - +0.0
5,225,645 - -1.8
5,225,687 + -1.0
5,225,906 + Pf6N2E2_4493 0.41 +1.0
5,225,906 + Pf6N2E2_4493 0.41 -0.6
5,225,906 + Pf6N2E2_4493 0.41 -0.8
5,225,906 + Pf6N2E2_4493 0.41 +1.0
5,225,907 - Pf6N2E2_4493 0.41 -0.2
5,225,998 + Pf6N2E2_4493 0.61 +0.0
5,225,998 + Pf6N2E2_4493 0.61 -0.6
5,225,999 - Pf6N2E2_4493 0.61 +1.0
5,225,999 - Pf6N2E2_4493 0.61 -0.6
5,226,009 + Pf6N2E2_4493 0.63 -1.2
5,226,009 + Pf6N2E2_4493 0.63 -0.1
5,226,009 + Pf6N2E2_4493 0.63 +0.0
5,226,009 + Pf6N2E2_4493 0.63 -0.6
5,226,009 + Pf6N2E2_4493 0.63 +1.0
5,226,009 + Pf6N2E2_4493 0.63 +0.0
5,226,010 - Pf6N2E2_4493 0.63 +0.0
5,226,010 - Pf6N2E2_4493 0.63 +1.0
5,226,010 - Pf6N2E2_4493 0.63 +1.0
5,226,010 - Pf6N2E2_4493 0.63 +1.0
5,226,069 + Pf6N2E2_4493 0.76 +1.0
5,226,069 + Pf6N2E2_4493 0.76 +0.0
5,226,070 - Pf6N2E2_4493 0.76 +1.0
5,226,070 - Pf6N2E2_4493 0.76 -1.2
5,226,070 - Pf6N2E2_4493 0.76 +1.0
5,226,070 - Pf6N2E2_4493 0.76 +2.0
5,226,249 + +0.0
5,226,249 + +0.3
5,226,249 + -1.6
5,226,249 + -1.2
5,226,249 + -0.2
5,226,249 + -0.3
5,226,249 + +0.0
5,226,249 + -0.8
5,226,249 + +0.0
5,226,250 - -0.7
5,226,250 - +1.0
5,226,250 - -1.6
5,226,250 - +1.0
5,226,259 + -1.5
5,226,343 + +0.0
5,226,343 + -1.0
5,226,343 + -0.6
5,226,433 + +0.0
5,227,049 - Pf6N2E2_4494 0.54 +0.0
5,227,049 - Pf6N2E2_4494 0.54 +0.0
5,227,064 - Pf6N2E2_4494 0.55 +0.0
5,227,417 + Pf6N2E2_4494 0.81 -2.7
5,227,417 + Pf6N2E2_4494 0.81 +0.0
5,227,418 - Pf6N2E2_4494 0.81 +1.0
5,227,549 + -1.0
5,227,549 + +0.0
5,227,550 - +1.0
5,227,550 - -1.6
5,227,739 + Pf6N2E2_4495 0.34 -0.5
5,227,739 + Pf6N2E2_4495 0.34 +0.0
5,227,739 + Pf6N2E2_4495 0.34 +1.0
5,227,739 + Pf6N2E2_4495 0.34 +1.0
5,227,739 + Pf6N2E2_4495 0.34 -0.6
5,227,758 - Pf6N2E2_4495 0.50 -0.3
5,227,796 + Pf6N2E2_4495 0.82 -1.0
5,227,796 + Pf6N2E2_4495 0.82 +0.0
5,227,796 + Pf6N2E2_4495 0.82 -0.5
5,227,796 + Pf6N2E2_4495 0.82 -0.4
5,227,796 + Pf6N2E2_4495 0.82 -0.2
5,227,796 + Pf6N2E2_4495 0.82 +1.0
5,227,796 + Pf6N2E2_4495 0.82 +0.0
5,227,796 + Pf6N2E2_4495 0.82 +1.0
5,227,796 + Pf6N2E2_4495 0.82 -0.6
5,227,796 + +1.0
5,227,797 - Pf6N2E2_4495 0.82 +1.0
5,227,797 - Pf6N2E2_4495 0.82 -1.8
5,227,797 - Pf6N2E2_4495 0.82 -0.6
5,227,797 - Pf6N2E2_4495 0.82 -0.9
5,227,797 - Pf6N2E2_4495 0.82 -1.0
5,227,797 - Pf6N2E2_4495 0.82 +1.0
5,227,797 - Pf6N2E2_4495 0.82 +0.0
5,227,815 + +1.0
5,227,815 + -0.3
5,227,815 + +1.0
5,227,816 - +0.0
5,227,816 - -0.4
5,228,046 + Pf6N2E2_4496 0.18 -0.4

Or see this region's nucleotide sequence