Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3342 and Pf6N2E2_3343 are separated by 319 nucleotides Pf6N2E2_3343 and Pf6N2E2_3344 overlap by 4 nucleotides Pf6N2E2_3344 and Pf6N2E2_3345 are separated by 64 nucleotides
Pf6N2E2_3342 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,868,491 to 3,870,521
_3342
Pf6N2E2_3343 - AmpE protein, at 3,870,841 to 3,871,677
_3343
Pf6N2E2_3344 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 3,871,674 to 3,872,234
_3344
Pf6N2E2_3345 - Thymidine phosphorylase (EC 2.4.2.4), at 3,872,299 to 3,874,512
_3345
Position (kb)
3870
3871
3872 Strain fitness (log2 ratio)
-2
-1
0
1 at 3870.300 kb on - strand, within Pf6N2E2_3342 at 3870.552 kb on + strand at 3870.552 kb on + strand at 3870.555 kb on - strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.605 kb on + strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.606 kb on - strand at 3870.607 kb on + strand at 3870.608 kb on - strand at 3870.612 kb on + strand at 3870.612 kb on + strand at 3870.612 kb on + strand at 3870.613 kb on - strand at 3870.613 kb on - strand at 3870.632 kb on + strand at 3870.633 kb on - strand at 3870.633 kb on - strand at 3870.637 kb on + strand at 3870.637 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.690 kb on + strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.691 kb on - strand at 3870.875 kb on + strand at 3870.991 kb on - strand, within Pf6N2E2_3343 at 3871.008 kb on + strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.009 kb on - strand, within Pf6N2E2_3343 at 3871.250 kb on + strand, within Pf6N2E2_3343 at 3871.251 kb on - strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.421 kb on + strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.464 kb on - strand, within Pf6N2E2_3343 at 3871.778 kb on + strand, within Pf6N2E2_3344 at 3871.779 kb on - strand, within Pf6N2E2_3344 at 3871.843 kb on + strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.844 kb on - strand, within Pf6N2E2_3344 at 3871.907 kb on + strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3871.908 kb on - strand, within Pf6N2E2_3344 at 3872.045 kb on - strand, within Pf6N2E2_3344 at 3872.126 kb on + strand, within Pf6N2E2_3344 at 3872.234 kb on + strand at 3872.235 kb on - strand at 3872.271 kb on + strand at 3872.272 kb on - strand at 3872.272 kb on - strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.465 kb on + strand at 3872.466 kb on - strand at 3872.466 kb on - strand at 3872.466 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 3,870,300 - Pf6N2E2_3342 0.89 -1.0 3,870,552 + -0.0 3,870,552 + -0.5 3,870,555 - -0.4 3,870,605 + -0.6 3,870,605 + -0.6 3,870,605 + -0.0 3,870,605 + -0.8 3,870,605 + -1.4 3,870,605 + -0.6 3,870,605 + -0.0 3,870,605 + -0.7 3,870,605 + -0.0 3,870,605 + -0.2 3,870,605 + -0.6 3,870,606 - +1.0 3,870,606 - -0.5 3,870,606 - -0.0 3,870,606 - +1.0 3,870,606 - -0.1 3,870,606 - -1.1 3,870,606 - -1.6 3,870,606 - -0.8 3,870,606 - -0.6 3,870,606 - -0.6 3,870,606 - -0.0 3,870,606 - -0.0 3,870,606 - +1.0 3,870,607 + -0.6 3,870,608 - +1.0 3,870,612 + -0.0 3,870,612 + -0.6 3,870,612 + +0.2 3,870,613 - -0.6 3,870,613 - +1.0 3,870,632 + +1.0 3,870,633 - -0.6 3,870,633 - -0.0 3,870,637 + -0.0 3,870,637 + +0.7 3,870,690 + -0.0 3,870,690 + -1.0 3,870,690 + -0.4 3,870,690 + -0.0 3,870,690 + -0.9 3,870,691 - -0.0 3,870,691 - +1.0 3,870,691 - -0.6 3,870,691 - -0.0 3,870,875 + -0.8 3,870,991 - Pf6N2E2_3343 0.18 -0.6 3,871,008 + Pf6N2E2_3343 0.20 -0.9 3,871,009 - Pf6N2E2_3343 0.20 -1.5 3,871,009 - Pf6N2E2_3343 0.20 -0.6 3,871,250 + Pf6N2E2_3343 0.49 -1.8 3,871,251 - Pf6N2E2_3343 0.49 -1.2 3,871,421 + Pf6N2E2_3343 0.69 -0.6 3,871,421 + Pf6N2E2_3343 0.69 +1.0 3,871,464 - Pf6N2E2_3343 0.74 +1.0 3,871,464 - Pf6N2E2_3343 0.74 +1.0 3,871,778 + Pf6N2E2_3344 0.19 -1.0 3,871,779 - Pf6N2E2_3344 0.19 -0.0 3,871,843 + Pf6N2E2_3344 0.30 +1.0 3,871,844 - Pf6N2E2_3344 0.30 +1.0 3,871,844 - Pf6N2E2_3344 0.30 -0.0 3,871,844 - Pf6N2E2_3344 0.30 -0.6 3,871,907 + Pf6N2E2_3344 0.42 +1.0 3,871,908 - Pf6N2E2_3344 0.42 +1.0 3,871,908 - Pf6N2E2_3344 0.42 -0.0 3,872,045 - Pf6N2E2_3344 0.66 +0.1 3,872,126 + Pf6N2E2_3344 0.81 -0.4 3,872,234 + -0.0 3,872,235 - -0.0 3,872,271 + -0.7 3,872,272 - -0.0 3,872,272 - -0.0 3,872,465 + +1.0 3,872,465 + -0.0 3,872,465 + -0.4 3,872,465 + -0.6 3,872,466 - +1.0 3,872,466 - +1.0 3,872,466 - -0.4
Or see this region's nucleotide sequence