Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_330 and Pf6N2E2_331 are separated by 265 nucleotides Pf6N2E2_331 and Pf6N2E2_332 are separated by 23 nucleotides Pf6N2E2_332 and Pf6N2E2_333 are separated by 17 nucleotides Pf6N2E2_333 and Pf6N2E2_334 are separated by 167 nucleotides
Pf6N2E2_330 - Mesenchymal stem cell protein DSCD75, at 398,698 to 399,249
_330
Pf6N2E2_331 - Putative translation initiation inhibitor, yjgF family, at 399,515 to 399,946
_331
Pf6N2E2_332 - putative nucleoside-diphosphate-sugar epimerase, at 399,970 to 400,848
_332
Pf6N2E2_333 - hypothetical protein, at 400,866 to 401,549
_333
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Position (kb) 399
400
401 Strain fitness (log2 ratio) -2
-1
0
1 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.075 kb on - strand, within Pf6N2E2_330 at 399.107 kb on + strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.283 kb on + strand at 399.284 kb on - strand at 399.307 kb on - strand at 399.392 kb on + strand at 399.494 kb on - strand at 399.624 kb on + strand, within Pf6N2E2_331 at 399.624 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.666 kb on + strand, within Pf6N2E2_331 at 399.938 kb on - strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.016 kb on + strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.017 kb on - strand at 400.075 kb on + strand, within Pf6N2E2_332 at 400.232 kb on + strand, within Pf6N2E2_332 at 400.415 kb on + strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.416 kb on - strand, within Pf6N2E2_332 at 400.419 kb on - strand, within Pf6N2E2_332 at 400.501 kb on - strand, within Pf6N2E2_332 at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.854 kb on + strand at 400.854 kb on + strand at 401.047 kb on + strand, within Pf6N2E2_333 at 401.047 kb on + strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.143 kb on + strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.192 kb on - strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.275 kb on - strand, within Pf6N2E2_333 at 401.438 kb on - strand, within Pf6N2E2_333 at 401.501 kb on - strand at 401.501 kb on - strand at 401.549 kb on + strand at 401.649 kb on + strand at 401.649 kb on + strand at 401.650 kb on - strand at 401.838 kb on + strand, within Pf6N2E2_334 at 401.841 kb on + strand, within Pf6N2E2_334
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 399,074 + Pf6N2E2_330 0.68 -1.1 399,074 + Pf6N2E2_330 0.68 +0.9 399,075 - Pf6N2E2_330 0.68 -1.6 399,107 + Pf6N2E2_330 0.74 -0.9 399,108 - Pf6N2E2_330 0.74 -0.8 399,108 - Pf6N2E2_330 0.74 -0.3 399,283 + -0.7 399,284 - -0.1 399,307 - -0.1 399,392 + -1.2 399,494 - +0.9 399,624 + Pf6N2E2_331 0.25 +0.4 399,624 + Pf6N2E2_331 0.25 +0.9 399,666 + Pf6N2E2_331 0.35 -0.3 399,666 + Pf6N2E2_331 0.35 -1.6 399,666 + Pf6N2E2_331 0.35 -0.1 399,938 - +0.5 400,016 + +0.9 400,016 + +0.9 400,016 + -0.1 400,016 + +0.9 400,016 + -0.6 400,017 - +0.9 400,017 - +0.9 400,017 - -0.7 400,017 - +0.9 400,017 - +0.5 400,075 + Pf6N2E2_332 0.12 -0.1 400,232 + Pf6N2E2_332 0.30 -1.1 400,415 + Pf6N2E2_332 0.51 +0.9 400,416 - Pf6N2E2_332 0.51 -0.1 400,416 - Pf6N2E2_332 0.51 -0.1 400,416 - Pf6N2E2_332 0.51 -2.1 400,416 - Pf6N2E2_332 0.51 +0.9 400,416 - Pf6N2E2_332 0.51 +0.9 400,416 - Pf6N2E2_332 0.51 -0.6 400,419 - Pf6N2E2_332 0.51 -2.2 400,501 - Pf6N2E2_332 0.60 -0.1 400,844 + -0.5 400,844 + +0.9 400,844 + +0.9 400,844 + +0.9 400,844 + -0.8 400,845 - +0.9 400,845 - -1.1 400,845 - +0.9 400,845 - +0.9 400,845 - -1.1 400,854 + +0.9 400,854 + -0.1 401,047 + Pf6N2E2_333 0.26 +0.9 401,047 + Pf6N2E2_333 0.26 -0.1 401,048 - Pf6N2E2_333 0.27 -0.1 401,048 - Pf6N2E2_333 0.27 -1.0 401,048 - Pf6N2E2_333 0.27 +0.9 401,048 - Pf6N2E2_333 0.27 +0.9 401,143 + Pf6N2E2_333 0.40 -0.1 401,144 - Pf6N2E2_333 0.41 -1.1 401,144 - Pf6N2E2_333 0.41 -0.1 401,144 - Pf6N2E2_333 0.41 -0.1 401,191 + Pf6N2E2_333 0.48 -1.9 401,191 + Pf6N2E2_333 0.48 -0.3 401,192 - Pf6N2E2_333 0.48 -0.6 401,274 + Pf6N2E2_333 0.60 +0.9 401,274 + Pf6N2E2_333 0.60 +0.9 401,274 + Pf6N2E2_333 0.60 -0.6 401,275 - Pf6N2E2_333 0.60 -0.1 401,438 - Pf6N2E2_333 0.84 -0.1 401,501 - -0.7 401,501 - +0.1 401,549 + -0.1 401,649 + -0.9 401,649 + +0.9 401,650 - -0.1 401,838 + Pf6N2E2_334 0.13 -1.1 401,841 + Pf6N2E2_334 0.13 +0.9
Or see this region's nucleotide sequence