Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2525 and Pf6N2E2_2526 are separated by 4 nucleotides Pf6N2E2_2526 and Pf6N2E2_2527 are separated by 167 nucleotides Pf6N2E2_2527 and Pf6N2E2_2528 are separated by 353 nucleotides
Pf6N2E2_2525 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 2,982,418 to 2,983,116
_2525
Pf6N2E2_2526 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 2,983,121 to 2,983,792
_2526
Pf6N2E2_2527 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,983,960 to 2,984,700
_2527
Pf6N2E2_2528 - GGDEF domain protein, at 2,985,054 to 2,985,980
_2528
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2983.114 kb on + strand at 2983.114 kb on + strand at 2983.115 kb on - strand at 2983.115 kb on - strand at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.682 kb on + strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.683 kb on - strand, within Pf6N2E2_2526 at 2983.688 kb on + strand, within Pf6N2E2_2526 at 2983.689 kb on - strand, within Pf6N2E2_2526 at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.942 kb on + strand at 2983.959 kb on + strand at 2983.959 kb on + strand at 2983.960 kb on - strand at 2984.057 kb on - strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.119 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.197 kb on + strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.198 kb on - strand, within Pf6N2E2_2527 at 2984.280 kb on + strand, within Pf6N2E2_2527 at 2984.355 kb on + strand, within Pf6N2E2_2527 at 2984.444 kb on - strand, within Pf6N2E2_2527 at 2984.455 kb on + strand, within Pf6N2E2_2527 at 2984.644 kb on + strand at 2984.727 kb on + strand at 2984.727 kb on + strand at 2984.728 kb on - strand at 2984.794 kb on + strand at 2984.795 kb on - strand at 2984.836 kb on + strand at 2984.902 kb on + strand at 2984.903 kb on - strand at 2984.903 kb on - strand at 2984.924 kb on + strand at 2984.924 kb on + strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.925 kb on - strand at 2984.985 kb on + strand at 2984.985 kb on + strand at 2984.986 kb on - strand at 2985.033 kb on + strand at 2985.033 kb on + strand at 2985.033 kb on + strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.034 kb on - strand at 2985.054 kb on - strand at 2985.274 kb on - strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.336 kb on + strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.337 kb on - strand, within Pf6N2E2_2528 at 2985.339 kb on - strand, within Pf6N2E2_2528
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 2,983,114 + -0.2 2,983,114 + +0.5 2,983,115 - -1.1 2,983,115 - -0.4 2,983,682 + Pf6N2E2_2526 0.83 +1.0 2,983,682 + Pf6N2E2_2526 0.83 +1.0 2,983,682 + Pf6N2E2_2526 0.83 +1.0 2,983,682 + Pf6N2E2_2526 0.83 -1.0 2,983,682 + Pf6N2E2_2526 0.83 -0.2 2,983,682 + Pf6N2E2_2526 0.83 +1.8 2,983,683 - Pf6N2E2_2526 0.84 +0.1 2,983,683 - Pf6N2E2_2526 0.84 +0.0 2,983,688 + Pf6N2E2_2526 0.84 +1.0 2,983,689 - Pf6N2E2_2526 0.85 -0.3 2,983,942 + -0.4 2,983,942 + -1.0 2,983,942 + +1.0 2,983,959 + +1.0 2,983,959 + -0.5 2,983,960 - +1.0 2,984,057 - Pf6N2E2_2527 0.13 -0.8 2,984,119 + Pf6N2E2_2527 0.21 -1.6 2,984,119 + Pf6N2E2_2527 0.21 -1.1 2,984,197 + Pf6N2E2_2527 0.32 -1.9 2,984,197 + Pf6N2E2_2527 0.32 -1.8 2,984,198 - Pf6N2E2_2527 0.32 -2.4 2,984,198 - Pf6N2E2_2527 0.32 -1.7 2,984,280 + Pf6N2E2_2527 0.43 -1.5 2,984,355 + Pf6N2E2_2527 0.53 -1.8 2,984,444 - Pf6N2E2_2527 0.65 -2.5 2,984,455 + Pf6N2E2_2527 0.67 -1.4 2,984,644 + -0.1 2,984,727 + +0.0 2,984,727 + +0.0 2,984,728 - -0.7 2,984,794 + +0.0 2,984,795 - +1.0 2,984,836 + +0.0 2,984,902 + -0.9 2,984,903 - -1.8 2,984,903 - -2.1 2,984,924 + -1.0 2,984,924 + +1.0 2,984,925 - -2.0 2,984,925 - +1.0 2,984,925 - +0.0 2,984,925 - +1.0 2,984,925 - +0.1 2,984,985 + +1.0 2,984,985 + +1.0 2,984,986 - +0.0 2,985,033 + +1.0 2,985,033 + -0.3 2,985,033 + -1.0 2,985,034 - +1.0 2,985,034 - -0.9 2,985,034 - -0.6 2,985,034 - +1.0 2,985,034 - -0.5 2,985,034 - +1.0 2,985,034 - -0.7 2,985,054 - +1.0 2,985,274 - Pf6N2E2_2528 0.24 +0.0 2,985,336 + Pf6N2E2_2528 0.30 +1.0 2,985,336 + Pf6N2E2_2528 0.30 +0.0 2,985,336 + Pf6N2E2_2528 0.30 -0.7 2,985,336 + Pf6N2E2_2528 0.30 +1.0 2,985,336 + Pf6N2E2_2528 0.30 +1.0 2,985,336 + Pf6N2E2_2528 0.30 +1.0 2,985,336 + Pf6N2E2_2528 0.30 -0.4 2,985,337 - Pf6N2E2_2528 0.31 -0.6 2,985,337 - Pf6N2E2_2528 0.31 -0.8 2,985,337 - Pf6N2E2_2528 0.31 -1.4 2,985,337 - Pf6N2E2_2528 0.31 -1.7 2,985,337 - Pf6N2E2_2528 0.31 -0.2 2,985,337 - Pf6N2E2_2528 0.31 +0.0 2,985,337 - Pf6N2E2_2528 0.31 +0.0 2,985,337 - Pf6N2E2_2528 0.31 +1.0 2,985,337 - Pf6N2E2_2528 0.31 +1.0 2,985,339 - Pf6N2E2_2528 0.31 -0.7
Or see this region's nucleotide sequence