Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_665

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_664 and Pf6N2E2_665 are separated by 2 nucleotidesPf6N2E2_665 and Pf6N2E2_666 are separated by 36 nucleotides Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025 _664 Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005 _665 Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058 _666 Position (kb) 770 771 772Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 769.432 kb on + strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664at 769.939 kb on + strandat 770.155 kb on - strand, within Pf6N2E2_665at 770.155 kb on - strand, within Pf6N2E2_665at 770.289 kb on + strand, within Pf6N2E2_665at 770.289 kb on + strand, within Pf6N2E2_665at 770.290 kb on - strand, within Pf6N2E2_665at 770.560 kb on - strand, within Pf6N2E2_665at 770.934 kb on + strandat 770.988 kb on + strandat 770.989 kb on - strandat 770.998 kb on - strandat 771.028 kb on + strandat 771.028 kb on + strandat 771.029 kb on - strandat 771.029 kb on - strandat 771.378 kb on + strand, within Pf6N2E2_666at 771.379 kb on - strand, within Pf6N2E2_666at 771.379 kb on - strand, within Pf6N2E2_666at 771.506 kb on - strand, within Pf6N2E2_666at 771.506 kb on - strand, within Pf6N2E2_666at 771.526 kb on - strand, within Pf6N2E2_666at 771.601 kb on - strand, within Pf6N2E2_666at 771.633 kb on + strand, within Pf6N2E2_666at 771.633 kb on + strand, within Pf6N2E2_666at 771.776 kb on - strand, within Pf6N2E2_666at 771.884 kb on + strand, within Pf6N2E2_666at 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.993 kb on + strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strandat 771.994 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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769,432 + Pf6N2E2_664 0.45 +0.8
769,433 - Pf6N2E2_664 0.45 -0.9
769,433 - Pf6N2E2_664 0.45 -1.5
769,939 + +0.8
770,155 - Pf6N2E2_665 0.13 -0.2
770,155 - Pf6N2E2_665 0.13 +0.8
770,289 + Pf6N2E2_665 0.27 -2.6
770,289 + Pf6N2E2_665 0.27 -2.0
770,290 - Pf6N2E2_665 0.27 +0.8
770,560 - Pf6N2E2_665 0.54 -0.8
770,934 + +0.8
770,988 + -0.8
770,989 - -3.8
770,998 - -0.2
771,028 + -1.7
771,028 + -1.2
771,029 - +0.1
771,029 - -0.2
771,378 + Pf6N2E2_666 0.33 +0.8
771,379 - Pf6N2E2_666 0.33 -0.2
771,379 - Pf6N2E2_666 0.33 -0.2
771,506 - Pf6N2E2_666 0.46 -0.8
771,506 - Pf6N2E2_666 0.46 -0.2
771,526 - Pf6N2E2_666 0.48 +0.8
771,601 - Pf6N2E2_666 0.55 -0.2
771,633 + Pf6N2E2_666 0.58 -0.7
771,633 + Pf6N2E2_666 0.58 -0.0
771,776 - Pf6N2E2_666 0.72 -2.2
771,884 + Pf6N2E2_666 0.83 +0.8
771,993 + -0.2
771,993 + -0.2
771,993 + +0.8
771,993 + +0.8
771,993 + -0.2
771,994 - -1.8
771,994 - +0.8
771,994 - +0.8
771,994 - +0.1
771,994 - -2.1

Or see this region's nucleotide sequence