Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_63 and Pf6N2E2_64 are separated by 11 nucleotides Pf6N2E2_64 and Pf6N2E2_65 are separated by 138 nucleotides Pf6N2E2_65 and Pf6N2E2_66 are separated by 66 nucleotides
Pf6N2E2_63 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 103,010 to 104,428
_63
Pf6N2E2_64 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 104,440 to 105,084
_64
Pf6N2E2_65 - SAM-dependent methyltransferase YafE (UbiE paralog), at 105,223 to 105,987
_65
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Position (kb) 105
106 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 104.318 kb on - strand at 104.350 kb on + strand at 104.602 kb on + strand, within Pf6N2E2_64 at 104.603 kb on - strand, within Pf6N2E2_64 at 104.660 kb on - strand, within Pf6N2E2_64 at 104.774 kb on - strand, within Pf6N2E2_64 at 105.092 kb on + strand at 105.251 kb on + strand at 105.252 kb on - strand at 105.252 kb on - strand at 105.283 kb on + strand at 105.329 kb on + strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.330 kb on - strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.436 kb on + strand, within Pf6N2E2_65 at 105.437 kb on - strand, within Pf6N2E2_65 at 105.697 kb on + strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.698 kb on - strand, within Pf6N2E2_65 at 105.801 kb on + strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.802 kb on - strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.829 kb on + strand, within Pf6N2E2_65 at 105.855 kb on + strand, within Pf6N2E2_65 at 105.954 kb on + strand at 105.955 kb on - strand at 106.013 kb on - strand at 106.013 kb on - strand at 106.147 kb on + strand at 106.147 kb on + strand at 106.148 kb on - strand at 106.148 kb on - strand at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 104,318 - -0.0 104,350 + -0.6 104,602 + Pf6N2E2_64 0.25 -0.6 104,603 - Pf6N2E2_64 0.25 -0.0 104,660 - Pf6N2E2_64 0.34 -4.6 104,774 - Pf6N2E2_64 0.52 -0.0 105,092 + -1.4 105,251 + -2.2 105,252 - -4.1 105,252 - -4.2 105,283 + +1.0 105,329 + Pf6N2E2_65 0.14 -1.6 105,330 - Pf6N2E2_65 0.14 -0.0 105,330 - Pf6N2E2_65 0.14 -2.4 105,330 - Pf6N2E2_65 0.14 -4.4 105,330 - Pf6N2E2_65 0.14 +1.0 105,330 - Pf6N2E2_65 0.14 -0.0 105,436 + Pf6N2E2_65 0.28 +1.0 105,436 + Pf6N2E2_65 0.28 -2.1 105,437 - Pf6N2E2_65 0.28 -1.4 105,697 + Pf6N2E2_65 0.62 +1.0 105,698 - Pf6N2E2_65 0.62 -0.0 105,698 - Pf6N2E2_65 0.62 +1.0 105,801 + Pf6N2E2_65 0.76 -0.4 105,802 - Pf6N2E2_65 0.76 -0.0 105,802 - Pf6N2E2_65 0.76 +1.0 105,829 + Pf6N2E2_65 0.79 -0.0 105,829 + Pf6N2E2_65 0.79 -1.8 105,855 + Pf6N2E2_65 0.83 -3.6 105,954 + -0.0 105,955 - +1.0 106,013 - +1.0 106,013 - -0.0 106,147 + +1.0 106,147 + -3.1 106,148 - +1.0 106,148 - +1.0 106,470 + Pf6N2E2_66 0.38 +1.0 106,470 + Pf6N2E2_66 0.38 +1.0 106,475 - Pf6N2E2_66 0.39 -0.0 106,475 - Pf6N2E2_66 0.39 -3.0 106,475 - Pf6N2E2_66 0.39 -0.0 106,529 - Pf6N2E2_66 0.44 +1.0 106,529 - Pf6N2E2_66 0.44 -0.0 106,543 + Pf6N2E2_66 0.45 -3.9 106,637 + Pf6N2E2_66 0.54 -0.0 106,717 + Pf6N2E2_66 0.61 -0.0 106,717 + Pf6N2E2_66 0.61 -0.0 106,717 + Pf6N2E2_66 0.61 +1.0 106,717 + Pf6N2E2_66 0.61 +1.0 106,717 + Pf6N2E2_66 0.61 +1.0 106,717 + Pf6N2E2_66 0.61 +1.0 106,718 - Pf6N2E2_66 0.61 -0.6 106,718 - Pf6N2E2_66 0.61 -7.1 106,718 - Pf6N2E2_66 0.61 -0.0 106,718 - Pf6N2E2_66 0.61 +1.0 106,718 - Pf6N2E2_66 0.61 -5.7 106,718 - Pf6N2E2_66 0.61 +1.0 106,718 - Pf6N2E2_66 0.61 -0.0 106,882 + Pf6N2E2_66 0.76 +1.0 106,882 + Pf6N2E2_66 0.76 -5.0 106,883 - Pf6N2E2_66 0.77 -2.2 106,883 - Pf6N2E2_66 0.77 +1.0 106,883 - Pf6N2E2_66 0.77 -2.2 106,883 - Pf6N2E2_66 0.77 -0.0 106,883 - Pf6N2E2_66 0.77 -1.6 106,975 + Pf6N2E2_66 0.85 -2.4
Or see this region's nucleotide sequence