Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_543

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_542 and Pf6N2E2_543 are separated by 450 nucleotidesPf6N2E2_543 and Pf6N2E2_544 are separated by 153 nucleotidesPf6N2E2_544 and Pf6N2E2_545 are separated by 24 nucleotides Pf6N2E2_542 - hypothetical protein, at 610,330 to 611,763 _542 Pf6N2E2_543 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 612,214 to 613,329 _543 Pf6N2E2_544 - Short-chain dehydrogenase/reductase SDR, at 613,483 to 614,250 _544 Pf6N2E2_545 - Predicted aminoglycoside phosphotransferase, at 614,275 to 615,342 _545 Position (kb) 612 613 614Strain fitness (log2 ratio) -3 -2 -1 0 1at 611.282 kb on + strand, within Pf6N2E2_542at 611.282 kb on + strand, within Pf6N2E2_542at 611.282 kb on + strand, within Pf6N2E2_542at 611.282 kb on + strand, within Pf6N2E2_542at 611.282 kb on + strand, within Pf6N2E2_542at 611.282 kb on + strand, within Pf6N2E2_542at 611.283 kb on - strand, within Pf6N2E2_542at 611.283 kb on - strand, within Pf6N2E2_542at 611.283 kb on - strand, within Pf6N2E2_542at 611.283 kb on - strand, within Pf6N2E2_542at 611.504 kb on - strand, within Pf6N2E2_542at 611.811 kb on + strandat 611.811 kb on + strandat 612.215 kb on + strandat 612.216 kb on - strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.217 kb on + strandat 612.218 kb on - strandat 612.218 kb on - strandat 612.218 kb on - strandat 612.218 kb on - strandat 612.218 kb on - strandat 612.218 kb on - strandat 612.378 kb on - strand, within Pf6N2E2_543at 612.378 kb on - strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.648 kb on + strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.649 kb on - strand, within Pf6N2E2_543at 612.935 kb on + strand, within Pf6N2E2_543at 612.935 kb on + strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.936 kb on - strand, within Pf6N2E2_543at 612.941 kb on + strand, within Pf6N2E2_543at 612.941 kb on + strand, within Pf6N2E2_543at 612.942 kb on - strand, within Pf6N2E2_543at 613.031 kb on + strand, within Pf6N2E2_543at 613.032 kb on - strand, within Pf6N2E2_543at 613.444 kb on + strandat 613.444 kb on + strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.445 kb on - strandat 613.529 kb on + strandat 613.529 kb on + strandat 613.553 kb on + strandat 613.553 kb on + strandat 613.554 kb on - strandat 613.769 kb on + strand, within Pf6N2E2_544at 613.769 kb on + strand, within Pf6N2E2_544at 613.769 kb on + strand, within Pf6N2E2_544at 613.770 kb on - strand, within Pf6N2E2_544at 613.773 kb on + strand, within Pf6N2E2_544at 613.774 kb on - strand, within Pf6N2E2_544at 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.266 kb on + strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strandat 614.267 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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611,282 + Pf6N2E2_542 0.66 -0.2
611,282 + Pf6N2E2_542 0.66 -0.8
611,282 + Pf6N2E2_542 0.66 -1.9
611,282 + Pf6N2E2_542 0.66 -1.7
611,282 + Pf6N2E2_542 0.66 +0.8
611,282 + Pf6N2E2_542 0.66 -0.2
611,283 - Pf6N2E2_542 0.66 -2.9
611,283 - Pf6N2E2_542 0.66 -2.3
611,283 - Pf6N2E2_542 0.66 -0.2
611,283 - Pf6N2E2_542 0.66 -0.2
611,504 - Pf6N2E2_542 0.82 +0.8
611,811 + +0.8
611,811 + -0.8
612,215 + +0.8
612,216 - -2.6
612,217 + -1.9
612,217 + -1.9
612,217 + +0.8
612,217 + +0.8
612,217 + -2.9
612,217 + -1.8
612,217 + +0.8
612,217 + -2.5
612,218 - -0.3
612,218 - -1.5
612,218 - -0.2
612,218 - -2.0
612,218 - +0.8
612,218 - -1.5
612,378 - Pf6N2E2_543 0.15 +0.8
612,378 - Pf6N2E2_543 0.15 +0.8
612,648 + Pf6N2E2_543 0.39 +0.8
612,648 + Pf6N2E2_543 0.39 +0.8
612,648 + Pf6N2E2_543 0.39 -0.4
612,648 + Pf6N2E2_543 0.39 +0.8
612,648 + Pf6N2E2_543 0.39 -1.5
612,648 + Pf6N2E2_543 0.39 +0.1
612,648 + Pf6N2E2_543 0.39 +0.8
612,649 - Pf6N2E2_543 0.39 +0.8
612,649 - Pf6N2E2_543 0.39 -0.2
612,649 - Pf6N2E2_543 0.39 +0.8
612,935 + Pf6N2E2_543 0.65 -2.3
612,935 + Pf6N2E2_543 0.65 -0.8
612,936 - Pf6N2E2_543 0.65 +0.8
612,936 - Pf6N2E2_543 0.65 +0.8
612,936 - Pf6N2E2_543 0.65 +0.8
612,941 + Pf6N2E2_543 0.65 -0.2
612,941 + Pf6N2E2_543 0.65 -0.2
612,942 - Pf6N2E2_543 0.65 -1.2
613,031 + Pf6N2E2_543 0.73 +0.8
613,032 - Pf6N2E2_543 0.73 +0.8
613,444 + +0.8
613,444 + -0.2
613,445 - -1.7
613,445 - +0.8
613,445 - -0.8
613,445 - +0.8
613,445 - -0.2
613,445 - -1.3
613,529 + -0.2
613,529 + -0.2
613,553 + +0.8
613,553 + +0.8
613,554 - -0.2
613,769 + Pf6N2E2_544 0.37 +0.8
613,769 + Pf6N2E2_544 0.37 -0.2
613,769 + Pf6N2E2_544 0.37 +0.8
613,770 - Pf6N2E2_544 0.37 +0.8
613,773 + Pf6N2E2_544 0.38 +0.8
613,774 - Pf6N2E2_544 0.38 +0.8
614,266 + -2.6
614,266 + -1.8
614,266 + -1.8
614,266 + -0.8
614,266 + -2.2
614,266 + -1.7
614,266 + +0.8
614,267 - +0.8
614,267 - -0.4
614,267 - +0.8
614,267 - -0.5
614,267 - -0.2

Or see this region's nucleotide sequence