Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_33 and Pf6N2E2_34 are separated by 56 nucleotides Pf6N2E2_34 and Pf6N2E2_35 are separated by 12 nucleotides
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Pf6N2E2_35 - Cytosine permease, at 74,316 to 75,587
_35
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33 at 73.130 kb on - strand at 73.162 kb on + strand at 73.163 kb on - strand at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 74.317 kb on + strand at 74.317 kb on + strand at 74.359 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.593 kb on + strand, within Pf6N2E2_35 at 74.594 kb on - strand, within Pf6N2E2_35 at 74.612 kb on - strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.822 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.139 kb on + strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.253 kb on + strand, within Pf6N2E2_35
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 72,255 + Pf6N2E2_33 0.26 -0.7 72,694 + Pf6N2E2_33 0.69 +1.0 72,695 - Pf6N2E2_33 0.70 -1.7 73,130 - +1.0 73,162 + -0.0 73,163 - +1.0 73,340 + Pf6N2E2_34 0.23 +1.0 73,458 - Pf6N2E2_34 0.32 +1.0 73,549 + Pf6N2E2_34 0.40 +1.0 73,550 - Pf6N2E2_34 0.40 -0.2 73,561 + Pf6N2E2_34 0.40 +1.0 73,562 - Pf6N2E2_34 0.41 +1.0 73,756 + Pf6N2E2_34 0.56 +1.0 73,756 + Pf6N2E2_34 0.56 -1.0 74,317 + -0.6 74,317 + +1.0 74,359 - -2.0 74,371 - -1.5 74,371 - -1.6 74,371 - -2.7 74,371 - +1.0 74,371 - +1.0 74,377 + +1.0 74,377 + -1.0 74,377 + +1.0 74,377 + -2.4 74,377 + -0.0 74,377 + -1.8 74,378 - -0.0 74,378 - -3.4 74,378 - +1.0 74,378 - +1.0 74,593 + Pf6N2E2_35 0.22 -2.0 74,594 - Pf6N2E2_35 0.22 -0.0 74,612 - Pf6N2E2_35 0.23 -1.4 74,716 + Pf6N2E2_35 0.31 -0.0 74,716 + Pf6N2E2_35 0.31 +1.0 74,716 + Pf6N2E2_35 0.31 -0.0 74,716 + Pf6N2E2_35 0.31 -1.0 74,716 + Pf6N2E2_35 0.31 +1.0 74,717 - Pf6N2E2_35 0.32 +1.0 74,717 - Pf6N2E2_35 0.32 -2.0 74,821 + Pf6N2E2_35 0.40 -1.0 74,821 + Pf6N2E2_35 0.40 -1.6 74,821 + Pf6N2E2_35 0.40 +1.0 74,821 + Pf6N2E2_35 0.40 +1.0 74,821 + Pf6N2E2_35 0.40 +1.0 74,822 - Pf6N2E2_35 0.40 +1.0 75,107 - Pf6N2E2_35 0.62 +1.0 75,107 - Pf6N2E2_35 0.62 -0.6 75,139 + Pf6N2E2_35 0.65 +1.0 75,140 - Pf6N2E2_35 0.65 -0.6 75,140 - Pf6N2E2_35 0.65 +1.0 75,211 + Pf6N2E2_35 0.70 +1.0 75,211 + Pf6N2E2_35 0.70 -0.6 75,211 + Pf6N2E2_35 0.70 -2.2 75,212 - Pf6N2E2_35 0.70 +0.2 75,212 - Pf6N2E2_35 0.70 -0.0 75,253 + Pf6N2E2_35 0.74 +1.0
Or see this region's nucleotide sequence