Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2620

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2619 and Pf6N2E2_2620 are separated by 284 nucleotidesPf6N2E2_2620 and Pf6N2E2_2621 are separated by 65 nucleotides Pf6N2E2_2619 - Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33), at 3,077,280 to 3,078,038 _2619 Pf6N2E2_2620 - O-antigen biosynthesis protein, at 3,078,323 to 3,078,571 _2620 Pf6N2E2_2621 - glycosyl transferase, group 2 family protein, at 3,078,637 to 3,081,894 _2621 Position (kb) 3078 3079Strain fitness (log2 ratio) -3 -2 -1 0 1at 3077.456 kb on + strand, within Pf6N2E2_2619at 3077.456 kb on + strand, within Pf6N2E2_2619at 3077.457 kb on - strand, within Pf6N2E2_2619at 3077.457 kb on - strand, within Pf6N2E2_2619at 3077.457 kb on - strand, within Pf6N2E2_2619at 3077.457 kb on - strand, within Pf6N2E2_2619at 3077.457 kb on - strand, within Pf6N2E2_2619at 3077.459 kb on - strand, within Pf6N2E2_2619at 3077.459 kb on - strand, within Pf6N2E2_2619at 3077.459 kb on - strand, within Pf6N2E2_2619at 3077.459 kb on - strand, within Pf6N2E2_2619at 3077.481 kb on + strand, within Pf6N2E2_2619at 3077.674 kb on + strand, within Pf6N2E2_2619at 3077.674 kb on + strand, within Pf6N2E2_2619at 3077.675 kb on - strand, within Pf6N2E2_2619at 3077.788 kb on + strand, within Pf6N2E2_2619at 3077.788 kb on + strand, within Pf6N2E2_2619at 3077.788 kb on + strand, within Pf6N2E2_2619at 3077.822 kb on + strand, within Pf6N2E2_2619at 3077.823 kb on - strand, within Pf6N2E2_2619at 3077.854 kb on + strand, within Pf6N2E2_2619at 3077.854 kb on + strand, within Pf6N2E2_2619at 3077.855 kb on - strand, within Pf6N2E2_2619at 3077.881 kb on + strand, within Pf6N2E2_2619at 3077.882 kb on - strand, within Pf6N2E2_2619at 3077.891 kb on - strand, within Pf6N2E2_2619at 3077.891 kb on - strand, within Pf6N2E2_2619at 3077.891 kb on - strand, within Pf6N2E2_2619at 3078.041 kb on + strandat 3078.042 kb on - strandat 3078.119 kb on - strandat 3078.170 kb on - strandat 3078.177 kb on + strandat 3078.178 kb on - strandat 3078.265 kb on - strandat 3078.282 kb on - strandat 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.378 kb on + strand, within Pf6N2E2_2620at 3078.379 kb on - strand, within Pf6N2E2_2620at 3078.379 kb on - strand, within Pf6N2E2_2620at 3078.420 kb on + strand, within Pf6N2E2_2620at 3078.421 kb on - strand, within Pf6N2E2_2620at 3078.421 kb on - strand, within Pf6N2E2_2620at 3078.424 kb on + strand, within Pf6N2E2_2620at 3078.424 kb on + strand, within Pf6N2E2_2620at 3078.496 kb on + strand, within Pf6N2E2_2620at 3078.660 kb on + strandat 3078.661 kb on - strandat 3078.697 kb on + strandat 3078.697 kb on + strandat 3078.698 kb on - strandat 3078.698 kb on - strandat 3078.698 kb on - strandat 3078.698 kb on - strandat 3078.841 kb on - strandat 3079.052 kb on + strand, within Pf6N2E2_2621at 3079.053 kb on - strand, within Pf6N2E2_2621at 3079.053 kb on - strand, within Pf6N2E2_2621at 3079.053 kb on - strand, within Pf6N2E2_2621at 3079.144 kb on + strand, within Pf6N2E2_2621at 3079.152 kb on + strand, within Pf6N2E2_2621at 3079.297 kb on + strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621at 3079.298 kb on - strand, within Pf6N2E2_2621

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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3,077,456 + Pf6N2E2_2619 0.23 +0.9
3,077,456 + Pf6N2E2_2619 0.23 +0.9
3,077,457 - Pf6N2E2_2619 0.23 +0.9
3,077,457 - Pf6N2E2_2619 0.23 -2.4
3,077,457 - Pf6N2E2_2619 0.23 -0.1
3,077,457 - Pf6N2E2_2619 0.23 -1.1
3,077,457 - Pf6N2E2_2619 0.23 -1.7
3,077,459 - Pf6N2E2_2619 0.24 -0.6
3,077,459 - Pf6N2E2_2619 0.24 +0.9
3,077,459 - Pf6N2E2_2619 0.24 -0.1
3,077,459 - Pf6N2E2_2619 0.24 +0.9
3,077,481 + Pf6N2E2_2619 0.26 -0.1
3,077,674 + Pf6N2E2_2619 0.52 -0.1
3,077,674 + Pf6N2E2_2619 0.52 +0.9
3,077,675 - Pf6N2E2_2619 0.52 -2.6
3,077,788 + Pf6N2E2_2619 0.67 -0.1
3,077,788 + Pf6N2E2_2619 0.67 +0.9
3,077,788 + Pf6N2E2_2619 0.67 -0.2
3,077,822 + Pf6N2E2_2619 0.71 +0.9
3,077,823 - Pf6N2E2_2619 0.72 +0.9
3,077,854 + Pf6N2E2_2619 0.76 -1.1
3,077,854 + Pf6N2E2_2619 0.76 +1.2
3,077,855 - Pf6N2E2_2619 0.76 -0.1
3,077,881 + Pf6N2E2_2619 0.79 +0.9
3,077,882 - Pf6N2E2_2619 0.79 -0.1
3,077,891 - Pf6N2E2_2619 0.81 -1.3
3,077,891 - Pf6N2E2_2619 0.81 +0.9
3,077,891 - Pf6N2E2_2619 0.81 -0.6
3,078,041 + -0.4
3,078,042 - +0.9
3,078,119 - +1.0
3,078,170 - -0.0
3,078,177 + +1.0
3,078,178 - +1.0
3,078,265 - +1.0
3,078,282 - +1.0
3,078,378 + Pf6N2E2_2620 0.22 +1.0
3,078,378 + Pf6N2E2_2620 0.22 -0.0
3,078,378 + Pf6N2E2_2620 0.22 +1.0
3,078,378 + Pf6N2E2_2620 0.22 -0.0
3,078,378 + Pf6N2E2_2620 0.22 -3.1
3,078,378 + Pf6N2E2_2620 0.22 -2.6
3,078,379 - Pf6N2E2_2620 0.22 -0.0
3,078,379 - Pf6N2E2_2620 0.22 -1.9
3,078,420 + Pf6N2E2_2620 0.39 +1.0
3,078,421 - Pf6N2E2_2620 0.39 +1.0
3,078,421 - Pf6N2E2_2620 0.39 -1.0
3,078,424 + Pf6N2E2_2620 0.41 -2.2
3,078,424 + Pf6N2E2_2620 0.41 -2.2
3,078,496 + Pf6N2E2_2620 0.69 -1.0
3,078,660 + -1.0
3,078,661 - -0.0
3,078,697 + -0.0
3,078,697 + +1.0
3,078,698 - -2.1
3,078,698 - +1.0
3,078,698 - -0.0
3,078,698 - -0.6
3,078,841 - -0.3
3,079,052 + Pf6N2E2_2621 0.13 +1.0
3,079,053 - Pf6N2E2_2621 0.13 -2.2
3,079,053 - Pf6N2E2_2621 0.13 -1.4
3,079,053 - Pf6N2E2_2621 0.13 -0.6
3,079,144 + Pf6N2E2_2621 0.16 -0.0
3,079,152 + Pf6N2E2_2621 0.16 -0.0
3,079,297 + Pf6N2E2_2621 0.20 -2.4
3,079,298 - Pf6N2E2_2621 0.20 -2.3
3,079,298 - Pf6N2E2_2621 0.20 -0.0
3,079,298 - Pf6N2E2_2621 0.20 +1.0
3,079,298 - Pf6N2E2_2621 0.20 -0.0
3,079,298 - Pf6N2E2_2621 0.20 +1.0
3,079,298 - Pf6N2E2_2621 0.20 -2.6
3,079,298 - Pf6N2E2_2621 0.20 -2.1

Or see this region's nucleotide sequence