Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2040 and Pf6N2E2_2041 are separated by 7 nucleotides Pf6N2E2_2041 and Pf6N2E2_2042 are separated by 190 nucleotides Pf6N2E2_2042 and Pf6N2E2_2043 are separated by 43 nucleotides
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Pf6N2E2_2041 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 2,430,586 to 2,431,362
_2041
Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508
_2042
Pf6N2E2_2043 - Twitching motility protein PilT, at 2,433,552 to 2,434,739
_2043
Position (kb)
2431
2432
2433
2434 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2430.559 kb on - strand at 2431.045 kb on + strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.292 kb on + strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.335 kb on + strand at 2431.336 kb on - strand at 2431.361 kb on - strand at 2431.498 kb on + strand at 2431.499 kb on - strand at 2431.502 kb on + strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2432.231 kb on + strand, within Pf6N2E2_2042 at 2432.232 kb on - strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.364 kb on - strand, within Pf6N2E2_2042 at 2432.483 kb on + strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.486 kb on + strand, within Pf6N2E2_2042 at 2432.486 kb on + strand, within Pf6N2E2_2042 at 2432.487 kb on - strand, within Pf6N2E2_2042 at 2432.487 kb on - strand, within Pf6N2E2_2042 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.749 kb on + strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.766 kb on - strand, within Pf6N2E2_2042 at 2433.029 kb on + strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.069 kb on - strand, within Pf6N2E2_2042 at 2433.367 kb on + strand at 2433.367 kb on + strand at 2433.368 kb on - strand at 2433.560 kb on + strand at 2433.614 kb on + strand at 2434.176 kb on - strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.320 kb on + strand, within Pf6N2E2_2043
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 2,430,559 - -2.2 2,431,045 + Pf6N2E2_2041 0.59 -0.7 2,431,046 - Pf6N2E2_2041 0.59 -1.1 2,431,046 - Pf6N2E2_2041 0.59 -2.2 2,431,046 - Pf6N2E2_2041 0.59 +0.9 2,431,046 - Pf6N2E2_2041 0.59 -0.1 2,431,046 - Pf6N2E2_2041 0.59 +0.9 2,431,292 + +0.9 2,431,293 - +0.9 2,431,293 - +0.9 2,431,293 - -0.8 2,431,335 + -3.1 2,431,336 - +0.9 2,431,361 - -0.1 2,431,498 + +0.9 2,431,499 - +0.9 2,431,502 + -0.1 2,431,503 - +0.9 2,431,503 - -0.1 2,431,503 - -0.7 2,431,503 - +0.9 2,431,524 + +0.9 2,431,524 + -0.1 2,431,524 + +0.9 2,431,525 - +0.9 2,431,525 - -0.9 2,431,525 - +0.9 2,432,231 + Pf6N2E2_2042 0.35 +0.9 2,432,232 - Pf6N2E2_2042 0.35 -2.0 2,432,354 + Pf6N2E2_2042 0.41 -0.7 2,432,354 + Pf6N2E2_2042 0.41 +0.9 2,432,354 + Pf6N2E2_2042 0.41 -0.7 2,432,354 + Pf6N2E2_2042 0.41 -2.0 2,432,355 - Pf6N2E2_2042 0.41 -0.1 2,432,355 - Pf6N2E2_2042 0.41 +0.9 2,432,355 - Pf6N2E2_2042 0.41 +0.9 2,432,364 - Pf6N2E2_2042 0.41 -0.1 2,432,483 + Pf6N2E2_2042 0.48 -0.1 2,432,484 - Pf6N2E2_2042 0.48 -0.7 2,432,484 - Pf6N2E2_2042 0.48 -2.1 2,432,484 - Pf6N2E2_2042 0.48 -0.7 2,432,484 - Pf6N2E2_2042 0.48 +0.9 2,432,486 + Pf6N2E2_2042 0.48 -1.7 2,432,486 + Pf6N2E2_2042 0.48 -0.1 2,432,487 - Pf6N2E2_2042 0.48 +0.9 2,432,487 - Pf6N2E2_2042 0.48 +0.9 2,432,559 - Pf6N2E2_2042 0.51 -1.7 2,432,559 - Pf6N2E2_2042 0.51 -1.4 2,432,749 + Pf6N2E2_2042 0.61 +0.9 2,432,750 - Pf6N2E2_2042 0.61 +0.9 2,432,750 - Pf6N2E2_2042 0.61 +0.9 2,432,750 - Pf6N2E2_2042 0.61 +0.9 2,432,753 + Pf6N2E2_2042 0.61 +0.9 2,432,753 + Pf6N2E2_2042 0.61 -0.1 2,432,753 + Pf6N2E2_2042 0.61 -0.7 2,432,753 + Pf6N2E2_2042 0.61 +0.9 2,432,753 + Pf6N2E2_2042 0.61 +0.9 2,432,753 + Pf6N2E2_2042 0.61 +0.9 2,432,754 - Pf6N2E2_2042 0.61 -2.2 2,432,754 - Pf6N2E2_2042 0.61 -2.1 2,432,754 - Pf6N2E2_2042 0.61 -0.1 2,432,754 - Pf6N2E2_2042 0.61 -0.7 2,432,754 - Pf6N2E2_2042 0.61 -2.0 2,432,754 - Pf6N2E2_2042 0.61 -0.1 2,432,759 + Pf6N2E2_2042 0.62 -0.1 2,432,759 + Pf6N2E2_2042 0.62 +0.9 2,432,759 + Pf6N2E2_2042 0.62 -2.7 2,432,759 + Pf6N2E2_2042 0.62 -1.4 2,432,759 + Pf6N2E2_2042 0.62 +0.9 2,432,759 + Pf6N2E2_2042 0.62 -0.1 2,432,760 - Pf6N2E2_2042 0.62 +0.9 2,432,760 - Pf6N2E2_2042 0.62 -0.4 2,432,760 - Pf6N2E2_2042 0.62 +0.9 2,432,760 - Pf6N2E2_2042 0.62 -1.7 2,432,760 - Pf6N2E2_2042 0.62 -0.7 2,432,766 - Pf6N2E2_2042 0.62 +0.9 2,433,029 + Pf6N2E2_2042 0.75 -0.1 2,433,030 - Pf6N2E2_2042 0.76 +0.9 2,433,030 - Pf6N2E2_2042 0.76 -0.1 2,433,069 - Pf6N2E2_2042 0.78 -1.4 2,433,367 + -2.6 2,433,367 + -2.2 2,433,368 - -0.4 2,433,560 + -1.6 2,433,614 + +0.9 2,434,176 - Pf6N2E2_2043 0.53 +0.9 2,434,177 + Pf6N2E2_2043 0.53 +0.9 2,434,177 + Pf6N2E2_2043 0.53 +0.9 2,434,177 + Pf6N2E2_2043 0.53 -0.1 2,434,177 + Pf6N2E2_2043 0.53 -1.1 2,434,178 - Pf6N2E2_2043 0.53 -0.1 2,434,178 - Pf6N2E2_2043 0.53 +0.9 2,434,178 - Pf6N2E2_2043 0.53 -0.6 2,434,320 + Pf6N2E2_2043 0.65 +0.9
Or see this region's nucleotide sequence