Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1647

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1646 and Pf6N2E2_1647 are separated by 21 nucleotidesPf6N2E2_1647 and Pf6N2E2_1648 overlap by 4 nucleotidesPf6N2E2_1648 and Pf6N2E2_1649 are separated by 31 nucleotides Pf6N2E2_1646 - Maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE, at 1,951,481 to 1,952,833 _1646 Pf6N2E2_1647 - Maltose/maltodextrin ABC transporter, permease protein MalF, at 1,952,855 to 1,953,820 _1647 Pf6N2E2_1648 - Maltose/maltodextrin ABC transporter, permease protein MalG, at 1,953,817 to 1,954,659 _1648 Pf6N2E2_1649 - Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19), at 1,954,691 to 1,955,845 _1649 Position (kb) 1952 1953 1954Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8 9 10 11at 1951.904 kb on + strand, within Pf6N2E2_1646at 1951.905 kb on - strand, within Pf6N2E2_1646at 1951.985 kb on + strand, within Pf6N2E2_1646at 1952.012 kb on + strand, within Pf6N2E2_1646at 1952.111 kb on + strand, within Pf6N2E2_1646at 1952.111 kb on + strand, within Pf6N2E2_1646at 1952.111 kb on + strand, within Pf6N2E2_1646at 1952.112 kb on - strand, within Pf6N2E2_1646at 1952.115 kb on + strand, within Pf6N2E2_1646at 1952.115 kb on + strand, within Pf6N2E2_1646at 1952.115 kb on + strand, within Pf6N2E2_1646at 1952.116 kb on - strand, within Pf6N2E2_1646at 1952.116 kb on - strand, within Pf6N2E2_1646at 1952.132 kb on + strand, within Pf6N2E2_1646at 1952.298 kb on - strand, within Pf6N2E2_1646at 1952.363 kb on + strand, within Pf6N2E2_1646at 1952.363 kb on + strand, within Pf6N2E2_1646at 1952.364 kb on - strand, within Pf6N2E2_1646at 1952.534 kb on + strand, within Pf6N2E2_1646at 1952.535 kb on - strand, within Pf6N2E2_1646at 1952.582 kb on + strand, within Pf6N2E2_1646at 1952.582 kb on + strand, within Pf6N2E2_1646at 1952.583 kb on - strand, within Pf6N2E2_1646at 1952.583 kb on - strand, within Pf6N2E2_1646at 1952.583 kb on - strand, within Pf6N2E2_1646at 1952.642 kb on + strand, within Pf6N2E2_1646at 1952.832 kb on - strandat 1952.869 kb on + strandat 1952.919 kb on + strandat 1952.970 kb on + strand, within Pf6N2E2_1647at 1952.971 kb on - strand, within Pf6N2E2_1647at 1953.080 kb on + strand, within Pf6N2E2_1647at 1953.081 kb on - strand, within Pf6N2E2_1647at 1953.081 kb on - strand, within Pf6N2E2_1647at 1953.503 kb on + strand, within Pf6N2E2_1647at 1953.638 kb on + strand, within Pf6N2E2_1647at 1953.638 kb on + strand, within Pf6N2E2_1647at 1953.638 kb on + strand, within Pf6N2E2_1647at 1953.683 kb on + strand, within Pf6N2E2_1647at 1953.684 kb on - strand, within Pf6N2E2_1647at 1953.780 kb on - strandat 1953.889 kb on + strandat 1953.907 kb on + strand, within Pf6N2E2_1648at 1953.908 kb on - strand, within Pf6N2E2_1648at 1953.910 kb on + strand, within Pf6N2E2_1648at 1953.910 kb on + strand, within Pf6N2E2_1648at 1953.961 kb on + strand, within Pf6N2E2_1648at 1953.991 kb on + strand, within Pf6N2E2_1648at 1954.099 kb on + strand, within Pf6N2E2_1648at 1954.225 kb on + strand, within Pf6N2E2_1648at 1954.649 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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1,951,904 + Pf6N2E2_1646 0.31 +0.8
1,951,905 - Pf6N2E2_1646 0.31 +0.8
1,951,985 + Pf6N2E2_1646 0.37 +0.8
1,952,012 + Pf6N2E2_1646 0.39 +0.8
1,952,111 + Pf6N2E2_1646 0.47 +0.8
1,952,111 + Pf6N2E2_1646 0.47 +0.8
1,952,111 + Pf6N2E2_1646 0.47 +0.8
1,952,112 - Pf6N2E2_1646 0.47 +0.8
1,952,115 + Pf6N2E2_1646 0.47 +10.4
1,952,115 + Pf6N2E2_1646 0.47 +0.8
1,952,115 + Pf6N2E2_1646 0.47 +0.8
1,952,116 - Pf6N2E2_1646 0.47 +10.1
1,952,116 - Pf6N2E2_1646 0.47 +11.3
1,952,132 + Pf6N2E2_1646 0.48 +0.8
1,952,298 - Pf6N2E2_1646 0.60 +9.4
1,952,363 + Pf6N2E2_1646 0.65 -0.2
1,952,363 + Pf6N2E2_1646 0.65 +0.8
1,952,364 - Pf6N2E2_1646 0.65 +0.8
1,952,534 + Pf6N2E2_1646 0.78 -0.2
1,952,535 - Pf6N2E2_1646 0.78 -0.7
1,952,582 + Pf6N2E2_1646 0.81 +9.6
1,952,582 + Pf6N2E2_1646 0.81 -0.2
1,952,583 - Pf6N2E2_1646 0.81 +0.8
1,952,583 - Pf6N2E2_1646 0.81 +9.1
1,952,583 - Pf6N2E2_1646 0.81 -0.2
1,952,642 + Pf6N2E2_1646 0.86 -1.2
1,952,832 - -0.8
1,952,869 + +10.0
1,952,919 + +0.8
1,952,970 + Pf6N2E2_1647 0.12 +0.8
1,952,971 - Pf6N2E2_1647 0.12 +10.6
1,953,080 + Pf6N2E2_1647 0.23 +9.7
1,953,081 - Pf6N2E2_1647 0.23 +10.7
1,953,081 - Pf6N2E2_1647 0.23 -0.2
1,953,503 + Pf6N2E2_1647 0.67 +0.8
1,953,638 + Pf6N2E2_1647 0.81 +0.8
1,953,638 + Pf6N2E2_1647 0.81 +0.8
1,953,638 + Pf6N2E2_1647 0.81 -0.8
1,953,683 + Pf6N2E2_1647 0.86 +10.4
1,953,684 - Pf6N2E2_1647 0.86 +9.7
1,953,780 - +0.8
1,953,889 + +0.8
1,953,907 + Pf6N2E2_1648 0.11 -1.2
1,953,908 - Pf6N2E2_1648 0.11 -0.2
1,953,910 + Pf6N2E2_1648 0.11 +9.8
1,953,910 + Pf6N2E2_1648 0.11 +0.8
1,953,961 + Pf6N2E2_1648 0.17 -0.8
1,953,991 + Pf6N2E2_1648 0.21 -0.2
1,954,099 + Pf6N2E2_1648 0.33 +9.4
1,954,225 + Pf6N2E2_1648 0.48 +0.8
1,954,649 + +0.8

Or see this region's nucleotide sequence