Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_135 and Pf6N2E2_136 are separated by 162 nucleotides Pf6N2E2_136 and Pf6N2E2_137 overlap by 1 nucleotides
Pf6N2E2_135 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 183,658 to 184,878
_135
Pf6N2E2_136 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 185,041 to 186,099
_136
Pf6N2E2_137 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 186,099 to 187,634
_137
Position (kb) 185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 184.106 kb on + strand, within Pf6N2E2_135 at 184.239 kb on - strand, within Pf6N2E2_135 at 184.607 kb on + strand, within Pf6N2E2_135 at 184.608 kb on - strand, within Pf6N2E2_135 at 184.705 kb on + strand, within Pf6N2E2_135 at 184.706 kb on - strand, within Pf6N2E2_135 at 184.706 kb on - strand, within Pf6N2E2_135 at 184.706 kb on - strand, within Pf6N2E2_135 at 184.721 kb on + strand, within Pf6N2E2_135 at 184.721 kb on + strand, within Pf6N2E2_135 at 184.722 kb on - strand, within Pf6N2E2_135 at 184.722 kb on - strand, within Pf6N2E2_135 at 184.722 kb on - strand, within Pf6N2E2_135 at 184.722 kb on - strand, within Pf6N2E2_135 at 184.722 kb on - strand, within Pf6N2E2_135 at 184.767 kb on - strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.878 kb on + strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.879 kb on - strand at 184.882 kb on + strand at 184.882 kb on + strand at 184.882 kb on + strand at 184.883 kb on - strand at 184.883 kb on - strand at 184.883 kb on - strand at 184.893 kb on - strand at 185.042 kb on + strand at 185.043 kb on - strand at 185.054 kb on + strand at 185.055 kb on - strand at 185.114 kb on + strand at 185.376 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.928 kb on - strand, within Pf6N2E2_136 at 186.545 kb on - strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 187.085 kb on - strand, within Pf6N2E2_137
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 184,106 + Pf6N2E2_135 0.37 -2.8 184,239 - Pf6N2E2_135 0.48 -0.0 184,607 + Pf6N2E2_135 0.78 -0.6 184,608 - Pf6N2E2_135 0.78 -0.0 184,705 + Pf6N2E2_135 0.86 -0.6 184,706 - Pf6N2E2_135 0.86 -1.0 184,706 - Pf6N2E2_135 0.86 -2.6 184,706 - Pf6N2E2_135 0.86 -2.2 184,721 + Pf6N2E2_135 0.87 -0.6 184,721 + Pf6N2E2_135 0.87 -0.8 184,722 - Pf6N2E2_135 0.87 +1.0 184,722 - Pf6N2E2_135 0.87 -1.9 184,722 - Pf6N2E2_135 0.87 +1.0 184,722 - Pf6N2E2_135 0.87 +1.0 184,722 - Pf6N2E2_135 0.87 +1.0 184,767 - +1.0 184,878 + -1.4 184,878 + -0.0 184,878 + -1.9 184,878 + +1.0 184,878 + -0.0 184,878 + -2.5 184,878 + -0.0 184,878 + -0.0 184,878 + +1.0 184,879 - +1.0 184,879 - -0.0 184,879 - +1.0 184,879 - +1.0 184,879 - +1.0 184,879 - +1.0 184,879 - -1.6 184,879 - -0.0 184,879 - +1.0 184,882 + -1.9 184,882 + +1.0 184,882 + +1.0 184,883 - +1.0 184,883 - -2.2 184,883 - -0.0 184,893 - -0.6 185,042 + -0.0 185,043 - -1.4 185,054 + -0.0 185,055 - +1.0 185,114 + +0.3 185,376 - Pf6N2E2_136 0.32 -0.5 185,557 - Pf6N2E2_136 0.49 +1.0 185,557 - Pf6N2E2_136 0.49 +1.0 185,928 - Pf6N2E2_136 0.84 +1.0 186,545 - Pf6N2E2_137 0.29 -0.0 186,547 + Pf6N2E2_137 0.29 -0.0 186,547 + Pf6N2E2_137 0.29 +0.4 186,547 + Pf6N2E2_137 0.29 -0.6 186,547 + Pf6N2E2_137 0.29 -0.6 186,548 - Pf6N2E2_137 0.29 -0.6 186,548 - Pf6N2E2_137 0.29 -1.6 186,548 - Pf6N2E2_137 0.29 +1.0 187,085 - Pf6N2E2_137 0.64 +1.0
Or see this region's nucleotide sequence