Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1221 and Pf6N2E2_1222 are separated by 148 nucleotides Pf6N2E2_1222 and Pf6N2E2_1223 are separated by 153 nucleotides Pf6N2E2_1223 and Pf6N2E2_1224 are separated by 114 nucleotides
Pf6N2E2_1221 - sodium/hydrogen exchanger family protein, at 1,427,660 to 1,428,997
_1221
Pf6N2E2_1222 - FIG00954310: hypothetical protein, at 1,429,146 to 1,429,682
_1222
Pf6N2E2_1223 - Amino acid transporters, at 1,429,836 to 1,430,066
_1223
Pf6N2E2_1224 - Two-component hybrid sensor and regulator, at 1,430,181 to 1,432,109
_1224
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.972 kb on - strand at 1428.999 kb on + strand at 1429.173 kb on - strand at 1429.174 kb on + strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.183 kb on + strand at 1429.183 kb on + strand at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.304 kb on - strand, within Pf6N2E2_1222 at 1429.335 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.672 kb on - strand at 1429.720 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.843 kb on - strand at 1429.946 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.985 kb on + strand, within Pf6N2E2_1223 at 1430.183 kb on - strand at 1430.183 kb on - strand at 1430.282 kb on - strand at 1430.407 kb on + strand, within Pf6N2E2_1224 at 1430.613 kb on - strand, within Pf6N2E2_1224 at 1430.798 kb on - strand, within Pf6N2E2_1224 at 1430.989 kb on - strand, within Pf6N2E2_1224 at 1431.027 kb on - strand, within Pf6N2E2_1224 at 1431.061 kb on + strand, within Pf6N2E2_1224
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 1,428,842 + Pf6N2E2_1221 0.88 -0.2 1,428,842 + Pf6N2E2_1221 0.88 +0.8 1,428,971 + +0.8 1,428,971 + +0.8 1,428,971 + +0.8 1,428,972 - -0.2 1,428,999 + +0.8 1,429,173 - +0.8 1,429,174 + -1.9 1,429,175 - +0.8 1,429,175 - -2.9 1,429,175 - -0.2 1,429,175 - -0.8 1,429,183 + -0.2 1,429,183 + +0.8 1,429,226 + Pf6N2E2_1222 0.15 +0.8 1,429,226 + Pf6N2E2_1222 0.15 -1.2 1,429,226 + Pf6N2E2_1222 0.15 +0.8 1,429,226 + Pf6N2E2_1222 0.15 +0.8 1,429,226 + Pf6N2E2_1222 0.15 -1.7 1,429,227 - Pf6N2E2_1222 0.15 -0.8 1,429,227 - Pf6N2E2_1222 0.15 -3.2 1,429,227 - Pf6N2E2_1222 0.15 -1.5 1,429,304 - Pf6N2E2_1222 0.29 -2.3 1,429,335 + Pf6N2E2_1222 0.35 -1.6 1,429,534 + Pf6N2E2_1222 0.72 -2.4 1,429,534 + Pf6N2E2_1222 0.72 +0.8 1,429,534 + Pf6N2E2_1222 0.72 -1.9 1,429,534 + Pf6N2E2_1222 0.72 -0.2 1,429,534 + Pf6N2E2_1222 0.72 -1.5 1,429,535 - Pf6N2E2_1222 0.72 +0.8 1,429,535 - Pf6N2E2_1222 0.72 +0.8 1,429,535 - Pf6N2E2_1222 0.72 -1.4 1,429,672 - +0.8 1,429,720 - +0.8 1,429,809 - -0.8 1,429,809 - -1.6 1,429,809 - +0.8 1,429,843 - -0.2 1,429,946 + Pf6N2E2_1223 0.48 +0.8 1,429,958 + Pf6N2E2_1223 0.53 +0.8 1,429,958 + Pf6N2E2_1223 0.53 -4.3 1,429,985 + Pf6N2E2_1223 0.65 +0.8 1,430,183 - -1.5 1,430,183 - -2.3 1,430,282 - -0.8 1,430,407 + Pf6N2E2_1224 0.12 -0.2 1,430,613 - Pf6N2E2_1224 0.22 +0.8 1,430,798 - Pf6N2E2_1224 0.32 +0.8 1,430,989 - Pf6N2E2_1224 0.42 -2.2 1,431,027 - Pf6N2E2_1224 0.44 +0.8 1,431,061 + Pf6N2E2_1224 0.46 -2.1
Or see this region's nucleotide sequence