Experiment: Ying_sugar7 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1100 and Pf6N2E2_1101 are separated by 54 nucleotides Pf6N2E2_1101 and Pf6N2E2_1102 are separated by 186 nucleotides
Pf6N2E2_1100 - L-rhamnose operon transcriptional activator RhaR, at 1,286,629 to 1,287,465
_1100
Pf6N2E2_1101 - Transporter, LysE family, at 1,287,520 to 1,288,122
_1101
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Position (kb)
1287
1288
1289 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1286.713 kb on + strand, within Pf6N2E2_1100 at 1286.714 kb on - strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.737 kb on + strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.738 kb on - strand, within Pf6N2E2_1100 at 1286.802 kb on + strand, within Pf6N2E2_1100 at 1286.802 kb on + strand, within Pf6N2E2_1100 at 1286.878 kb on + strand, within Pf6N2E2_1100 at 1286.878 kb on + strand, within Pf6N2E2_1100 at 1286.890 kb on + strand, within Pf6N2E2_1100 at 1287.073 kb on - strand, within Pf6N2E2_1100 at 1287.286 kb on + strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.287 kb on - strand, within Pf6N2E2_1100 at 1287.434 kb on - strand at 1287.531 kb on - strand at 1287.603 kb on + strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.604 kb on - strand, within Pf6N2E2_1101 at 1287.752 kb on + strand, within Pf6N2E2_1101 at 1288.009 kb on + strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.010 kb on - strand, within Pf6N2E2_1101 at 1288.300 kb on + strand at 1288.322 kb on + strand at 1288.336 kb on - strand at 1288.344 kb on - strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.349 kb on + strand at 1288.350 kb on - strand at 1288.350 kb on - strand at 1288.393 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.442 kb on + strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.443 kb on - strand at 1288.524 kb on - strand, within Pf6N2E2_1102 at 1288.629 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102 at 1288.877 kb on + strand, within Pf6N2E2_1102
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2 remove 1,286,713 + Pf6N2E2_1100 0.10 +0.8 1,286,714 - Pf6N2E2_1100 0.10 -0.2 1,286,737 + Pf6N2E2_1100 0.13 -0.2 1,286,737 + Pf6N2E2_1100 0.13 -0.2 1,286,737 + Pf6N2E2_1100 0.13 +0.8 1,286,738 - Pf6N2E2_1100 0.13 +0.8 1,286,738 - Pf6N2E2_1100 0.13 +0.8 1,286,738 - Pf6N2E2_1100 0.13 -0.2 1,286,802 + Pf6N2E2_1100 0.21 +0.8 1,286,802 + Pf6N2E2_1100 0.21 +0.8 1,286,878 + Pf6N2E2_1100 0.30 -0.2 1,286,878 + Pf6N2E2_1100 0.30 +0.8 1,286,890 + Pf6N2E2_1100 0.31 -2.7 1,287,073 - Pf6N2E2_1100 0.53 +0.8 1,287,286 + Pf6N2E2_1100 0.78 +0.8 1,287,287 - Pf6N2E2_1100 0.79 +0.8 1,287,287 - Pf6N2E2_1100 0.79 -1.3 1,287,287 - Pf6N2E2_1100 0.79 +0.8 1,287,287 - Pf6N2E2_1100 0.79 -1.5 1,287,287 - Pf6N2E2_1100 0.79 +0.8 1,287,287 - Pf6N2E2_1100 0.79 -1.0 1,287,434 - -1.0 1,287,531 - -0.2 1,287,603 + Pf6N2E2_1101 0.14 -0.2 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 -3.3 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,604 - Pf6N2E2_1101 0.14 -0.2 1,287,604 - Pf6N2E2_1101 0.14 +0.8 1,287,752 + Pf6N2E2_1101 0.38 -0.8 1,288,009 + Pf6N2E2_1101 0.81 -0.2 1,288,010 - Pf6N2E2_1101 0.81 -2.4 1,288,010 - Pf6N2E2_1101 0.81 -1.5 1,288,010 - Pf6N2E2_1101 0.81 -0.2 1,288,300 + -0.2 1,288,322 + -0.8 1,288,336 - +0.8 1,288,344 - +0.8 1,288,349 + +0.8 1,288,349 + +0.8 1,288,349 + -1.5 1,288,349 + +0.8 1,288,350 - +0.8 1,288,350 - -0.2 1,288,393 + +0.8 1,288,442 + -0.2 1,288,442 + +0.8 1,288,442 + +0.8 1,288,443 - +0.8 1,288,443 - -0.2 1,288,443 - -2.8 1,288,524 - Pf6N2E2_1102 0.15 +0.8 1,288,629 + Pf6N2E2_1102 0.22 +0.8 1,288,877 + Pf6N2E2_1102 0.40 +0.5 1,288,877 + Pf6N2E2_1102 0.40 -1.5 1,288,877 + Pf6N2E2_1102 0.40 -1.2
Or see this region's nucleotide sequence