Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_10

Experiment: Ying_sugar7 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_9 and Pf6N2E2_10 are separated by 37 nucleotidesPf6N2E2_10 and Pf6N2E2_11 are separated by 353 nucleotidesPf6N2E2_11 and Pf6N2E2_12 are separated by 144 nucleotides Pf6N2E2_9 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 34,541 to 38,686 _9 Pf6N2E2_10 - PvdE, pyoverdine ABC export system, fused ATPase and permease components, at 38,724 to 40,382 _10 Pf6N2E2_11 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 40,736 to 40,888 _11 Pf6N2E2_12 - PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV), at 41,033 to 41,908 _12 Position (kb) 38 39 40 41Strain fitness (log2 ratio) -3 -2 -1 0 1at 37.733 kb on + strand, within Pf6N2E2_9at 37.919 kb on + strand, within Pf6N2E2_9at 37.919 kb on + strand, within Pf6N2E2_9at 37.920 kb on - strand, within Pf6N2E2_9at 37.934 kb on - strand, within Pf6N2E2_9at 37.934 kb on - strand, within Pf6N2E2_9at 38.049 kb on + strand, within Pf6N2E2_9at 38.049 kb on + strand, within Pf6N2E2_9at 38.073 kb on + strand, within Pf6N2E2_9at 38.073 kb on + strand, within Pf6N2E2_9at 38.074 kb on - strand, within Pf6N2E2_9at 38.082 kb on + strand, within Pf6N2E2_9at 38.083 kb on - strand, within Pf6N2E2_9at 38.527 kb on - strandat 38.585 kb on + strandat 38.672 kb on + strandat 38.673 kb on - strandat 38.804 kb on - strandat 38.870 kb on + strandat 38.872 kb on + strandat 38.872 kb on + strandat 38.873 kb on - strandat 39.113 kb on - strand, within Pf6N2E2_10at 39.115 kb on + strand, within Pf6N2E2_10at 39.215 kb on - strand, within Pf6N2E2_10at 39.538 kb on + strand, within Pf6N2E2_10at 39.538 kb on + strand, within Pf6N2E2_10at 39.538 kb on + strand, within Pf6N2E2_10at 39.701 kb on - strand, within Pf6N2E2_10at 39.794 kb on - strand, within Pf6N2E2_10at 39.865 kb on + strand, within Pf6N2E2_10at 39.865 kb on + strand, within Pf6N2E2_10at 39.866 kb on - strand, within Pf6N2E2_10at 40.106 kb on + strand, within Pf6N2E2_10at 40.106 kb on + strand, within Pf6N2E2_10at 40.106 kb on + strand, within Pf6N2E2_10at 40.106 kb on + strand, within Pf6N2E2_10at 40.106 kb on + strand, within Pf6N2E2_10at 40.311 kb on + strandat 40.311 kb on + strandat 40.312 kb on - strandat 40.481 kb on + strandat 40.481 kb on + strandat 40.532 kb on + strandat 40.532 kb on + strandat 40.533 kb on - strandat 40.575 kb on - strandat 40.575 kb on - strandat 40.575 kb on - strandat 40.737 kb on + strandat 40.737 kb on + strandat 40.737 kb on + strandat 40.737 kb on + strandat 40.737 kb on + strandat 40.737 kb on + strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.738 kb on - strandat 40.742 kb on - strandat 40.742 kb on - strandat 40.911 kb on + strandat 40.912 kb on - strandat 40.912 kb on - strandat 40.912 kb on - strandat 41.034 kb on + strandat 41.034 kb on + strandat 41.034 kb on + strandat 41.034 kb on + strandat 41.034 kb on + strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.035 kb on - strandat 41.038 kb on + strandat 41.208 kb on + strand, within Pf6N2E2_12at 41.209 kb on - strand, within Pf6N2E2_12at 41.209 kb on - strand, within Pf6N2E2_12at 41.221 kb on - strand, within Pf6N2E2_12at 41.221 kb on - strand, within Pf6N2E2_12at 41.241 kb on + strand, within Pf6N2E2_12at 41.262 kb on + strand, within Pf6N2E2_12at 41.331 kb on + strand, within Pf6N2E2_12at 41.359 kb on - strand, within Pf6N2E2_12

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 2
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37,733 + Pf6N2E2_9 0.77 -1.5
37,919 + Pf6N2E2_9 0.81 +1.0
37,919 + Pf6N2E2_9 0.81 -2.2
37,920 - Pf6N2E2_9 0.82 +1.0
37,934 - Pf6N2E2_9 0.82 -2.7
37,934 - Pf6N2E2_9 0.82 -0.0
38,049 + Pf6N2E2_9 0.85 -3.3
38,049 + Pf6N2E2_9 0.85 -0.0
38,073 + Pf6N2E2_9 0.85 -1.9
38,073 + Pf6N2E2_9 0.85 -1.7
38,074 - Pf6N2E2_9 0.85 -0.0
38,082 + Pf6N2E2_9 0.85 +1.0
38,083 - Pf6N2E2_9 0.85 -0.3
38,527 - -0.0
38,585 + -1.2
38,672 + +1.0
38,673 - -2.0
38,804 - +1.0
38,870 + -0.0
38,872 + +1.0
38,872 + -0.0
38,873 - +1.0
39,113 - Pf6N2E2_10 0.23 +1.0
39,115 + Pf6N2E2_10 0.24 -0.0
39,215 - Pf6N2E2_10 0.30 +1.0
39,538 + Pf6N2E2_10 0.49 +1.0
39,538 + Pf6N2E2_10 0.49 -0.6
39,538 + Pf6N2E2_10 0.49 +1.0
39,701 - Pf6N2E2_10 0.59 -2.4
39,794 - Pf6N2E2_10 0.64 -2.4
39,865 + Pf6N2E2_10 0.69 -0.0
39,865 + Pf6N2E2_10 0.69 +1.0
39,866 - Pf6N2E2_10 0.69 -0.8
40,106 + Pf6N2E2_10 0.83 +1.0
40,106 + Pf6N2E2_10 0.83 -2.2
40,106 + Pf6N2E2_10 0.83 -1.2
40,106 + Pf6N2E2_10 0.83 -0.0
40,106 + Pf6N2E2_10 0.83 +1.0
40,311 + +1.0
40,311 + -1.2
40,312 - -1.4
40,481 + -0.3
40,481 + -0.0
40,532 + -0.0
40,532 + -0.6
40,533 - +1.0
40,575 - -0.0
40,575 - -0.0
40,575 - -1.4
40,737 + -2.1
40,737 + +1.0
40,737 + -1.8
40,737 + -0.0
40,737 + -2.1
40,737 + -1.4
40,738 - +1.0
40,738 - +1.0
40,738 - +1.0
40,738 - +1.0
40,738 - -1.8
40,738 - +1.0
40,738 - -1.0
40,738 - +1.0
40,742 - -0.6
40,742 - -0.0
40,911 + +1.0
40,912 - -0.6
40,912 - -0.6
40,912 - -0.0
41,034 + +1.0
41,034 + +1.0
41,034 + -0.6
41,034 + +1.0
41,034 + -1.9
41,035 - -1.8
41,035 - -0.0
41,035 - +1.0
41,035 - -0.6
41,035 - -2.7
41,035 - -0.0
41,035 - -2.2
41,035 - +1.0
41,035 - -0.4
41,035 - -2.6
41,035 - -0.0
41,038 + -1.0
41,208 + Pf6N2E2_12 0.20 +1.0
41,209 - Pf6N2E2_12 0.20 +1.0
41,209 - Pf6N2E2_12 0.20 +1.0
41,221 - Pf6N2E2_12 0.21 -1.8
41,221 - Pf6N2E2_12 0.21 +1.0
41,241 + Pf6N2E2_12 0.24 +1.0
41,262 + Pf6N2E2_12 0.26 -2.3
41,331 + Pf6N2E2_12 0.34 +1.0
41,359 - Pf6N2E2_12 0.37 -2.1

Or see this region's nucleotide sequence