Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_976 and Pf6N2E2_977 overlap by 4 nucleotides Pf6N2E2_977 and Pf6N2E2_978 overlap by 4 nucleotides Pf6N2E2_978 and Pf6N2E2_979 are separated by 14 nucleotides Pf6N2E2_979 and Pf6N2E2_980 are separated by 159 nucleotides
Pf6N2E2_976 - FIG141751: hypothetical protein in PFGI-1-like cluster, at 1,144,141 to 1,145,328
_976
Pf6N2E2_977 - FIG004780: hypothetical protein in PFGI-1-like cluster, at 1,145,325 to 1,145,873
_977
Pf6N2E2_978 - Protein with ParB-like nuclease domain in PFGI-1-like cluster, at 1,145,870 to 1,147,453
_978
Pf6N2E2_979 - hypothetical protein, at 1,147,468 to 1,147,602
_979
Pf6N2E2_980 - Chromosome partitioning ATPase in PFGI-1-like cluster, ParA-like, at 1,147,762 to 1,148,598
_980
Position (kb)
1145
1146
1147
1148 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1144.910 kb on + strand, within Pf6N2E2_976 at 1144.910 kb on + strand, within Pf6N2E2_976 at 1144.911 kb on - strand, within Pf6N2E2_976 at 1144.925 kb on - strand, within Pf6N2E2_976 at 1144.925 kb on - strand, within Pf6N2E2_976 at 1144.958 kb on - strand, within Pf6N2E2_976 at 1144.961 kb on - strand, within Pf6N2E2_976 at 1144.961 kb on - strand, within Pf6N2E2_976 at 1145.052 kb on - strand, within Pf6N2E2_976 at 1145.114 kb on - strand, within Pf6N2E2_976 at 1145.114 kb on - strand, within Pf6N2E2_976 at 1145.114 kb on - strand, within Pf6N2E2_976 at 1145.114 kb on - strand, within Pf6N2E2_976 at 1145.269 kb on + strand at 1145.270 kb on - strand at 1145.270 kb on - strand at 1145.270 kb on - strand at 1145.337 kb on - strand at 1145.337 kb on - strand at 1145.342 kb on - strand at 1145.342 kb on - strand at 1145.442 kb on + strand, within Pf6N2E2_977 at 1145.442 kb on + strand, within Pf6N2E2_977 at 1145.517 kb on + strand, within Pf6N2E2_977 at 1145.575 kb on + strand, within Pf6N2E2_977 at 1145.575 kb on + strand, within Pf6N2E2_977 at 1145.585 kb on + strand, within Pf6N2E2_977 at 1145.586 kb on - strand, within Pf6N2E2_977 at 1145.631 kb on - strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.758 kb on + strand, within Pf6N2E2_977 at 1145.759 kb on - strand, within Pf6N2E2_977 at 1145.906 kb on - strand at 1145.906 kb on - strand at 1145.912 kb on + strand at 1145.957 kb on - strand at 1146.027 kb on + strand at 1146.039 kb on - strand, within Pf6N2E2_978 at 1146.125 kb on + strand, within Pf6N2E2_978 at 1146.125 kb on + strand, within Pf6N2E2_978 at 1146.125 kb on + strand at 1146.125 kb on + strand, within Pf6N2E2_978 at 1146.126 kb on - strand, within Pf6N2E2_978 at 1146.419 kb on + strand, within Pf6N2E2_978 at 1146.419 kb on + strand, within Pf6N2E2_978 at 1146.420 kb on - strand, within Pf6N2E2_978 at 1146.575 kb on + strand, within Pf6N2E2_978 at 1146.648 kb on - strand, within Pf6N2E2_978 at 1146.836 kb on + strand, within Pf6N2E2_978 at 1146.837 kb on - strand, within Pf6N2E2_978 at 1146.872 kb on + strand, within Pf6N2E2_978 at 1146.872 kb on + strand, within Pf6N2E2_978 at 1147.203 kb on + strand, within Pf6N2E2_978 at 1147.203 kb on + strand, within Pf6N2E2_978 at 1147.204 kb on - strand, within Pf6N2E2_978 at 1147.204 kb on - strand, within Pf6N2E2_978 at 1147.281 kb on + strand, within Pf6N2E2_978 at 1147.348 kb on + strand at 1147.349 kb on - strand at 1147.355 kb on - strand at 1147.355 kb on - strand at 1147.588 kb on + strand, within Pf6N2E2_979 at 1147.659 kb on + strand at 1147.659 kb on + strand at 1147.660 kb on - strand at 1147.660 kb on - strand at 1147.743 kb on + strand at 1147.743 kb on + strand at 1147.791 kb on + strand at 1147.791 kb on + strand at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.951 kb on + strand, within Pf6N2E2_980 at 1147.952 kb on - strand, within Pf6N2E2_980 at 1147.961 kb on - strand, within Pf6N2E2_980 at 1148.053 kb on + strand, within Pf6N2E2_980 at 1148.163 kb on + strand, within Pf6N2E2_980 at 1148.163 kb on + strand, within Pf6N2E2_980 at 1148.164 kb on - strand, within Pf6N2E2_980 at 1148.212 kb on + strand, within Pf6N2E2_980 at 1148.212 kb on + strand, within Pf6N2E2_980 at 1148.217 kb on + strand, within Pf6N2E2_980 at 1148.217 kb on + strand, within Pf6N2E2_980 at 1148.218 kb on - strand, within Pf6N2E2_980 at 1148.390 kb on - strand, within Pf6N2E2_980
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 1,144,910 + Pf6N2E2_976 0.65 +0.8 1,144,910 + Pf6N2E2_976 0.65 -0.2 1,144,911 - Pf6N2E2_976 0.65 +0.0 1,144,925 - Pf6N2E2_976 0.66 -2.1 1,144,925 - Pf6N2E2_976 0.66 -0.2 1,144,958 - Pf6N2E2_976 0.69 -0.2 1,144,961 - Pf6N2E2_976 0.69 +0.8 1,144,961 - Pf6N2E2_976 0.69 +0.8 1,145,052 - Pf6N2E2_976 0.77 +0.8 1,145,114 - Pf6N2E2_976 0.82 +0.8 1,145,114 - Pf6N2E2_976 0.82 +1.8 1,145,114 - Pf6N2E2_976 0.82 +0.8 1,145,114 - Pf6N2E2_976 0.82 -0.2 1,145,269 + +0.8 1,145,270 - +0.8 1,145,270 - +0.8 1,145,270 - -0.2 1,145,337 - +0.0 1,145,337 - +0.8 1,145,342 - +0.8 1,145,342 - -0.2 1,145,442 + Pf6N2E2_977 0.21 -0.2 1,145,442 + Pf6N2E2_977 0.21 -1.9 1,145,517 + Pf6N2E2_977 0.35 +0.8 1,145,575 + Pf6N2E2_977 0.46 -0.2 1,145,575 + Pf6N2E2_977 0.46 -0.2 1,145,585 + Pf6N2E2_977 0.47 +0.8 1,145,586 - Pf6N2E2_977 0.48 -0.2 1,145,631 - Pf6N2E2_977 0.56 -0.6 1,145,758 + Pf6N2E2_977 0.79 -3.1 1,145,758 + Pf6N2E2_977 0.79 +0.2 1,145,758 + Pf6N2E2_977 0.79 -1.0 1,145,758 + Pf6N2E2_977 0.79 +0.8 1,145,758 + Pf6N2E2_977 0.79 +0.8 1,145,758 + Pf6N2E2_977 0.79 +0.8 1,145,758 + Pf6N2E2_977 0.79 +0.8 1,145,758 + Pf6N2E2_977 0.79 -0.2 1,145,759 - Pf6N2E2_977 0.79 +0.8 1,145,906 - +0.8 1,145,906 - +0.8 1,145,912 + +0.8 1,145,957 - +0.8 1,146,027 + -1.5 1,146,039 - Pf6N2E2_978 0.11 -3.9 1,146,125 + Pf6N2E2_978 0.16 -1.2 1,146,125 + Pf6N2E2_978 0.16 -0.5 1,146,125 + +0.8 1,146,125 + Pf6N2E2_978 0.16 +0.8 1,146,126 - Pf6N2E2_978 0.16 -0.5 1,146,419 + Pf6N2E2_978 0.35 +0.8 1,146,419 + Pf6N2E2_978 0.35 -1.9 1,146,420 - Pf6N2E2_978 0.35 +0.8 1,146,575 + Pf6N2E2_978 0.45 +0.8 1,146,648 - Pf6N2E2_978 0.49 -2.7 1,146,836 + Pf6N2E2_978 0.61 +0.8 1,146,837 - Pf6N2E2_978 0.61 -0.2 1,146,872 + Pf6N2E2_978 0.63 +0.8 1,146,872 + Pf6N2E2_978 0.63 +0.8 1,147,203 + Pf6N2E2_978 0.84 -0.2 1,147,203 + Pf6N2E2_978 0.84 -0.2 1,147,204 - Pf6N2E2_978 0.84 -0.2 1,147,204 - Pf6N2E2_978 0.84 -2.2 1,147,281 + Pf6N2E2_978 0.89 -0.2 1,147,348 + +0.8 1,147,349 - +0.8 1,147,355 - +0.8 1,147,355 - +0.8 1,147,588 + Pf6N2E2_979 0.89 +0.8 1,147,659 + +0.8 1,147,659 + +0.8 1,147,660 - +1.2 1,147,660 - -0.2 1,147,743 + +0.8 1,147,743 + -1.5 1,147,791 + +0.8 1,147,791 + +0.8 1,147,951 + Pf6N2E2_980 0.23 +0.8 1,147,951 + Pf6N2E2_980 0.23 +0.8 1,147,951 + Pf6N2E2_980 0.23 +0.8 1,147,951 + Pf6N2E2_980 0.23 +0.8 1,147,952 - Pf6N2E2_980 0.23 -2.9 1,147,961 - Pf6N2E2_980 0.24 +0.8 1,148,053 + Pf6N2E2_980 0.35 +0.8 1,148,163 + Pf6N2E2_980 0.48 +0.8 1,148,163 + Pf6N2E2_980 0.48 +0.8 1,148,164 - Pf6N2E2_980 0.48 +0.8 1,148,212 + Pf6N2E2_980 0.54 +0.8 1,148,212 + Pf6N2E2_980 0.54 +0.8 1,148,217 + Pf6N2E2_980 0.54 -0.8 1,148,217 + Pf6N2E2_980 0.54 +0.8 1,148,218 - Pf6N2E2_980 0.54 -1.7 1,148,390 - Pf6N2E2_980 0.75 -0.2
Or see this region's nucleotide sequence