Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_613

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_612 and Pf6N2E2_613 are separated by 118 nucleotidesPf6N2E2_613 and Pf6N2E2_614 overlap by 14 nucleotides Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data), at 695,832 to 697,412 _612 Pf6N2E2_613 - lipoprotein, putative, at 697,531 to 697,869 _613 Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031 _614 Position (kb) 697 698Strain fitness (log2 ratio) -3 -2 -1 0 1at 696.735 kb on - strand, within Pf6N2E2_612at 696.858 kb on + strand, within Pf6N2E2_612at 697.705 kb on - strand, within Pf6N2E2_613at 697.985 kb on + strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 697.986 kb on - strand, within Pf6N2E2_614at 698.153 kb on + strand, within Pf6N2E2_614at 698.153 kb on + strand, within Pf6N2E2_614at 698.266 kb on + strand, within Pf6N2E2_614at 698.266 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.270 kb on + strand, within Pf6N2E2_614at 698.271 kb on - strand, within Pf6N2E2_614at 698.271 kb on - strand, within Pf6N2E2_614at 698.438 kb on + strand, within Pf6N2E2_614at 698.438 kb on + strand, within Pf6N2E2_614at 698.438 kb on + strand, within Pf6N2E2_614at 698.438 kb on + strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.439 kb on - strand, within Pf6N2E2_614at 698.472 kb on + strand, within Pf6N2E2_614at 698.472 kb on + strand, within Pf6N2E2_614at 698.472 kb on + strand, within Pf6N2E2_614at 698.472 kb on + strand, within Pf6N2E2_614at 698.472 kb on + strand, within Pf6N2E2_614at 698.473 kb on - strand, within Pf6N2E2_614at 698.473 kb on - strand, within Pf6N2E2_614at 698.756 kb on + strand, within Pf6N2E2_614at 698.756 kb on + strand, within Pf6N2E2_614

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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696,735 - Pf6N2E2_612 0.57 -0.1
696,858 + Pf6N2E2_612 0.65 +0.9
697,705 - Pf6N2E2_613 0.51 -3.7
697,985 + Pf6N2E2_614 0.11 +0.9
697,986 - Pf6N2E2_614 0.11 +0.9
697,986 - Pf6N2E2_614 0.11 +0.9
697,986 - Pf6N2E2_614 0.11 -2.5
697,986 - Pf6N2E2_614 0.11 +0.9
698,153 + Pf6N2E2_614 0.25 +0.9
698,153 + Pf6N2E2_614 0.25 +0.9
698,266 + Pf6N2E2_614 0.35 -0.1
698,266 + Pf6N2E2_614 0.35 +0.9
698,270 + Pf6N2E2_614 0.35 +0.9
698,270 + Pf6N2E2_614 0.35 +0.9
698,270 + Pf6N2E2_614 0.35 +0.9
698,271 - Pf6N2E2_614 0.35 -0.7
698,271 - Pf6N2E2_614 0.35 -2.3
698,438 + Pf6N2E2_614 0.49 +0.9
698,438 + Pf6N2E2_614 0.49 +0.9
698,438 + Pf6N2E2_614 0.49 -0.7
698,438 + Pf6N2E2_614 0.49 -0.8
698,439 - Pf6N2E2_614 0.50 +0.9
698,439 - Pf6N2E2_614 0.50 +0.9
698,439 - Pf6N2E2_614 0.50 -0.1
698,439 - Pf6N2E2_614 0.50 +0.1
698,439 - Pf6N2E2_614 0.50 -1.6
698,439 - Pf6N2E2_614 0.50 +0.9
698,472 + Pf6N2E2_614 0.52 +0.9
698,472 + Pf6N2E2_614 0.52 -0.7
698,472 + Pf6N2E2_614 0.52 +0.9
698,472 + Pf6N2E2_614 0.52 -0.7
698,472 + Pf6N2E2_614 0.52 -3.1
698,473 - Pf6N2E2_614 0.52 -1.5
698,473 - Pf6N2E2_614 0.52 +0.9
698,756 + Pf6N2E2_614 0.77 -0.1
698,756 + Pf6N2E2_614 0.77 -2.0

Or see this region's nucleotide sequence