Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5264

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5262 and Pf6N2E2_5263 are separated by 121 nucleotidesPf6N2E2_5263 and Pf6N2E2_5264 are separated by 215 nucleotidesPf6N2E2_5264 and Pf6N2E2_5265 overlap by 33 nucleotides Pf6N2E2_5262 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 6,057,501 to 6,057,986 _5262 Pf6N2E2_5263 - Glutathione peroxidase family protein, at 6,058,108 to 6,058,590 _5263 Pf6N2E2_5264 - hypothetical protein, at 6,058,806 to 6,058,940 _5264 Pf6N2E2_5265 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 6,058,908 to 6,060,011 _5265 Position (kb) 6058 6059Strain fitness (log2 ratio) -2 -1 0 1at 6057.997 kb on - strandat 6058.044 kb on + strandat 6058.058 kb on + strandat 6058.058 kb on + strandat 6058.059 kb on - strandat 6058.059 kb on - strandat 6058.128 kb on + strandat 6058.128 kb on + strandat 6058.129 kb on - strandat 6058.232 kb on - strand, within Pf6N2E2_5263at 6058.253 kb on - strand, within Pf6N2E2_5263at 6058.253 kb on - strand, within Pf6N2E2_5263at 6058.253 kb on - strand, within Pf6N2E2_5263at 6058.261 kb on + strand, within Pf6N2E2_5263at 6058.261 kb on + strand, within Pf6N2E2_5263at 6058.261 kb on + strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.262 kb on - strand, within Pf6N2E2_5263at 6058.470 kb on + strand, within Pf6N2E2_5263at 6058.471 kb on - strand, within Pf6N2E2_5263at 6058.471 kb on - strand, within Pf6N2E2_5263at 6058.672 kb on + strandat 6058.690 kb on + strandat 6058.807 kb on + strandat 6058.808 kb on - strandat 6058.808 kb on - strandat 6058.808 kb on - strandat 6058.815 kb on - strandat 6058.830 kb on + strand, within Pf6N2E2_5264at 6058.837 kb on - strand, within Pf6N2E2_5264at 6058.859 kb on + strand, within Pf6N2E2_5264at 6058.859 kb on + strand, within Pf6N2E2_5264at 6058.859 kb on + strand, within Pf6N2E2_5264at 6058.859 kb on + strand, within Pf6N2E2_5264at 6058.860 kb on - strand, within Pf6N2E2_5264at 6058.860 kb on - strand, within Pf6N2E2_5264at 6058.860 kb on - strand, within Pf6N2E2_5264at 6058.863 kb on - strand, within Pf6N2E2_5264at 6058.915 kb on + strandat 6058.916 kb on - strandat 6058.916 kb on - strandat 6059.034 kb on + strand, within Pf6N2E2_5265at 6059.034 kb on + strand, within Pf6N2E2_5265at 6059.035 kb on - strand, within Pf6N2E2_5265at 6059.035 kb on - strand, within Pf6N2E2_5265at 6059.038 kb on - strand, within Pf6N2E2_5265at 6059.136 kb on + strand, within Pf6N2E2_5265at 6059.136 kb on + strand, within Pf6N2E2_5265at 6059.137 kb on - strand, within Pf6N2E2_5265at 6059.183 kb on - strand, within Pf6N2E2_5265at 6059.598 kb on + strand, within Pf6N2E2_5265at 6059.598 kb on + strand, within Pf6N2E2_5265at 6059.673 kb on - strand, within Pf6N2E2_5265

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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6,057,997 - +0.1
6,058,044 + +1.1
6,058,058 + -1.2
6,058,058 + +0.1
6,058,059 - -0.5
6,058,059 - -1.2
6,058,128 + -0.4
6,058,128 + +1.1
6,058,129 - +1.1
6,058,232 - Pf6N2E2_5263 0.26 -0.5
6,058,253 - Pf6N2E2_5263 0.30 +1.1
6,058,253 - Pf6N2E2_5263 0.30 +0.1
6,058,253 - Pf6N2E2_5263 0.30 -0.9
6,058,261 + Pf6N2E2_5263 0.32 +1.1
6,058,261 + Pf6N2E2_5263 0.32 +1.1
6,058,261 + Pf6N2E2_5263 0.32 +1.1
6,058,262 - Pf6N2E2_5263 0.32 +0.1
6,058,262 - Pf6N2E2_5263 0.32 +0.1
6,058,262 - Pf6N2E2_5263 0.32 -1.8
6,058,262 - Pf6N2E2_5263 0.32 +1.1
6,058,262 - Pf6N2E2_5263 0.32 +1.1
6,058,262 - Pf6N2E2_5263 0.32 +1.1
6,058,262 - Pf6N2E2_5263 0.32 +0.1
6,058,262 - Pf6N2E2_5263 0.32 +0.1
6,058,470 + Pf6N2E2_5263 0.75 -2.3
6,058,471 - Pf6N2E2_5263 0.75 +1.1
6,058,471 - Pf6N2E2_5263 0.75 +1.1
6,058,672 + +1.1
6,058,690 + +0.1
6,058,807 + -0.9
6,058,808 - +0.1
6,058,808 - +1.1
6,058,808 - -1.2
6,058,815 - +0.1
6,058,830 + Pf6N2E2_5264 0.18 +1.1
6,058,837 - Pf6N2E2_5264 0.23 -0.5
6,058,859 + Pf6N2E2_5264 0.39 -2.0
6,058,859 + Pf6N2E2_5264 0.39 +1.1
6,058,859 + Pf6N2E2_5264 0.39 +0.1
6,058,859 + Pf6N2E2_5264 0.39 +1.1
6,058,860 - Pf6N2E2_5264 0.40 -2.5
6,058,860 - Pf6N2E2_5264 0.40 -1.9
6,058,860 - Pf6N2E2_5264 0.40 -1.5
6,058,863 - Pf6N2E2_5264 0.42 -1.5
6,058,915 + -1.8
6,058,916 - +0.1
6,058,916 - +1.1
6,059,034 + Pf6N2E2_5265 0.11 +0.1
6,059,034 + Pf6N2E2_5265 0.11 -1.0
6,059,035 - Pf6N2E2_5265 0.12 -1.6
6,059,035 - Pf6N2E2_5265 0.12 +1.1
6,059,038 - Pf6N2E2_5265 0.12 -1.5
6,059,136 + Pf6N2E2_5265 0.21 -0.5
6,059,136 + Pf6N2E2_5265 0.21 +1.1
6,059,137 - Pf6N2E2_5265 0.21 -2.3
6,059,183 - Pf6N2E2_5265 0.25 +1.1
6,059,598 + Pf6N2E2_5265 0.62 -1.4
6,059,598 + Pf6N2E2_5265 0.62 +1.1
6,059,673 - Pf6N2E2_5265 0.69 -1.6

Or see this region's nucleotide sequence