Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotides Pf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotides Pf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides
Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593
_519
Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720
_520
Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888
_521
Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866
_522
Position (kb)
590
591 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 589.998 kb on - strand, within Pf6N2E2_519 at 590.121 kb on - strand, within Pf6N2E2_519 at 590.261 kb on + strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.262 kb on - strand, within Pf6N2E2_519 at 590.429 kb on + strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.430 kb on - strand, within Pf6N2E2_519 at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.724 kb on + strand at 590.725 kb on - strand at 590.729 kb on - strand at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.763 kb on + strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.764 kb on - strand, within Pf6N2E2_521 at 590.907 kb on + strand at 591.060 kb on + strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.061 kb on - strand, within Pf6N2E2_522 at 591.478 kb on - strand, within Pf6N2E2_522 at 591.658 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522 at 591.770 kb on + strand, within Pf6N2E2_522
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 589,998 - Pf6N2E2_519 0.43 +0.9 590,121 - Pf6N2E2_519 0.55 -1.4 590,261 + Pf6N2E2_519 0.68 -0.7 590,262 - Pf6N2E2_519 0.68 -2.6 590,262 - Pf6N2E2_519 0.68 +0.9 590,262 - Pf6N2E2_519 0.68 +0.9 590,429 + Pf6N2E2_519 0.84 +0.9 590,430 - Pf6N2E2_519 0.84 +0.9 590,430 - Pf6N2E2_519 0.84 -0.1 590,430 - Pf6N2E2_519 0.84 +0.9 590,430 - Pf6N2E2_519 0.84 -0.1 590,430 - Pf6N2E2_519 0.84 +0.3 590,430 - Pf6N2E2_519 0.84 +0.9 590,724 + -0.1 590,724 + -0.7 590,724 + +0.9 590,724 + +0.9 590,725 - +0.9 590,729 - +0.9 590,763 + Pf6N2E2_521 0.18 +0.9 590,763 + Pf6N2E2_521 0.18 +0.9 590,763 + Pf6N2E2_521 0.18 -2.7 590,763 + Pf6N2E2_521 0.18 +0.9 590,764 - Pf6N2E2_521 0.18 -4.0 590,764 - Pf6N2E2_521 0.18 -0.7 590,907 + -0.1 591,060 + Pf6N2E2_522 0.13 +0.9 591,061 - Pf6N2E2_522 0.13 -0.1 591,061 - Pf6N2E2_522 0.13 +0.9 591,478 - Pf6N2E2_522 0.58 +0.9 591,658 + Pf6N2E2_522 0.77 +0.9 591,770 + Pf6N2E2_522 0.90 -2.8 591,770 + Pf6N2E2_522 0.90 -0.1
Or see this region's nucleotide sequence