Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_521

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotidesPf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotidesPf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593 _519 Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720 _520 Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888 _521 Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866 _522 Position (kb) 590 591Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 589.998 kb on - strand, within Pf6N2E2_519at 590.121 kb on - strand, within Pf6N2E2_519at 590.261 kb on + strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.429 kb on + strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.725 kb on - strandat 590.729 kb on - strandat 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.907 kb on + strandat 591.060 kb on + strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.478 kb on - strand, within Pf6N2E2_522at 591.658 kb on + strand, within Pf6N2E2_522at 591.770 kb on + strand, within Pf6N2E2_522at 591.770 kb on + strand, within Pf6N2E2_522

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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589,998 - Pf6N2E2_519 0.43 +0.9
590,121 - Pf6N2E2_519 0.55 -1.4
590,261 + Pf6N2E2_519 0.68 -0.7
590,262 - Pf6N2E2_519 0.68 -2.6
590,262 - Pf6N2E2_519 0.68 +0.9
590,262 - Pf6N2E2_519 0.68 +0.9
590,429 + Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 -0.1
590,430 - Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 -0.1
590,430 - Pf6N2E2_519 0.84 +0.3
590,430 - Pf6N2E2_519 0.84 +0.9
590,724 + -0.1
590,724 + -0.7
590,724 + +0.9
590,724 + +0.9
590,725 - +0.9
590,729 - +0.9
590,763 + Pf6N2E2_521 0.18 +0.9
590,763 + Pf6N2E2_521 0.18 +0.9
590,763 + Pf6N2E2_521 0.18 -2.7
590,763 + Pf6N2E2_521 0.18 +0.9
590,764 - Pf6N2E2_521 0.18 -4.0
590,764 - Pf6N2E2_521 0.18 -0.7
590,907 + -0.1
591,060 + Pf6N2E2_522 0.13 +0.9
591,061 - Pf6N2E2_522 0.13 -0.1
591,061 - Pf6N2E2_522 0.13 +0.9
591,478 - Pf6N2E2_522 0.58 +0.9
591,658 + Pf6N2E2_522 0.77 +0.9
591,770 + Pf6N2E2_522 0.90 -2.8
591,770 + Pf6N2E2_522 0.90 -0.1

Or see this region's nucleotide sequence