Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_518

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_517 and Pf6N2E2_518 are separated by 31 nucleotidesPf6N2E2_518 and Pf6N2E2_519 are separated by 39 nucleotides Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) (from data), at 586,528 to 588,459 _517 Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501 _518 Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593 _519 Position (kb) 588 589 590Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 587.512 kb on - strand, within Pf6N2E2_517at 587.675 kb on - strand, within Pf6N2E2_517at 587.686 kb on + strand, within Pf6N2E2_517at 587.847 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.899 kb on + strandat 587.899 kb on + strand, within Pf6N2E2_517at 587.899 kb on + strand, within Pf6N2E2_517at 587.900 kb on - strand, within Pf6N2E2_517at 587.911 kb on + strand, within Pf6N2E2_517at 587.912 kb on - strand, within Pf6N2E2_517at 587.933 kb on - strand, within Pf6N2E2_517at 587.986 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.227 kb on - strand, within Pf6N2E2_517at 588.241 kb on + strand, within Pf6N2E2_517at 588.242 kb on - strand, within Pf6N2E2_517at 588.242 kb on - strand, within Pf6N2E2_517at 588.352 kb on + strandat 588.352 kb on + strandat 588.352 kb on + strandat 588.353 kb on - strandat 588.353 kb on - strandat 588.454 kb on + strandat 588.472 kb on + strandat 588.473 kb on - strandat 588.473 kb on - strandat 588.473 kb on - strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.541 kb on + strandat 588.653 kb on + strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.188 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518at 589.529 kb on + strandat 589.529 kb on + strandat 589.529 kb on + strandat 589.529 kb on + strandat 589.530 kb on - strandat 589.530 kb on - strandat 589.530 kb on - strandat 589.618 kb on + strandat 589.618 kb on + strandat 589.619 kb on - strandat 589.619 kb on - strandat 589.998 kb on - strand, within Pf6N2E2_519at 590.121 kb on - strand, within Pf6N2E2_519at 590.261 kb on + strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.429 kb on + strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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587,512 - Pf6N2E2_517 0.51 +0.9
587,675 - Pf6N2E2_517 0.59 -0.7
587,686 + Pf6N2E2_517 0.60 +0.9
587,847 + Pf6N2E2_517 0.68 +0.9
587,895 + Pf6N2E2_517 0.71 -0.1
587,895 + Pf6N2E2_517 0.71 +0.9
587,895 + Pf6N2E2_517 0.71 -1.8
587,895 + Pf6N2E2_517 0.71 +0.9
587,895 + Pf6N2E2_517 0.71 -0.7
587,896 - Pf6N2E2_517 0.71 +0.9
587,896 - Pf6N2E2_517 0.71 -0.1
587,896 - Pf6N2E2_517 0.71 -1.1
587,896 - Pf6N2E2_517 0.71 +0.9
587,896 - Pf6N2E2_517 0.71 +0.9
587,896 - Pf6N2E2_517 0.71 +0.9
587,896 - Pf6N2E2_517 0.71 -0.1
587,899 + -0.1
587,899 + Pf6N2E2_517 0.71 -0.1
587,899 + Pf6N2E2_517 0.71 +0.9
587,900 - Pf6N2E2_517 0.71 -2.6
587,911 + Pf6N2E2_517 0.72 -1.1
587,912 - Pf6N2E2_517 0.72 -1.9
587,933 - Pf6N2E2_517 0.73 +0.9
587,986 + Pf6N2E2_517 0.75 +0.9
588,010 + Pf6N2E2_517 0.77 -2.0
588,010 + Pf6N2E2_517 0.77 -0.1
588,010 + Pf6N2E2_517 0.77 -0.1
588,010 + Pf6N2E2_517 0.77 -1.1
588,010 + Pf6N2E2_517 0.77 -3.8
588,011 - Pf6N2E2_517 0.77 -0.1
588,011 - Pf6N2E2_517 0.77 +0.9
588,011 - Pf6N2E2_517 0.77 -0.1
588,011 - Pf6N2E2_517 0.77 -0.7
588,011 - Pf6N2E2_517 0.77 +0.9
588,227 - Pf6N2E2_517 0.88 -0.1
588,241 + Pf6N2E2_517 0.89 -0.8
588,242 - Pf6N2E2_517 0.89 -0.7
588,242 - Pf6N2E2_517 0.89 +0.9
588,352 + -3.7
588,352 + -1.4
588,352 + -0.1
588,353 - -1.1
588,353 - +0.9
588,454 + -0.7
588,472 + -2.1
588,473 - +0.9
588,473 - -0.1
588,473 - +0.9
588,488 + +0.9
588,488 + +0.9
588,488 + -0.7
588,488 + +0.9
588,488 + -2.9
588,488 + -2.6
588,488 + +0.9
588,489 - -0.1
588,489 - -0.7
588,489 - +0.9
588,489 - +0.9
588,489 - +0.9
588,489 - +0.9
588,489 - -0.1
588,489 - -3.8
588,489 - -3.3
588,541 + -1.4
588,653 + Pf6N2E2_518 0.16 -0.1
588,876 - Pf6N2E2_518 0.38 -1.7
588,876 - Pf6N2E2_518 0.38 -3.8
588,876 - Pf6N2E2_518 0.38 +0.9
588,981 - Pf6N2E2_518 0.48 +0.9
588,981 - Pf6N2E2_518 0.48 +0.9
588,981 - Pf6N2E2_518 0.48 -0.1
589,055 + Pf6N2E2_518 0.56 +0.9
589,055 + Pf6N2E2_518 0.56 -0.1
589,055 + Pf6N2E2_518 0.56 -0.4
589,055 + Pf6N2E2_518 0.56 -2.8
589,055 + Pf6N2E2_518 0.56 -0.1
589,055 + Pf6N2E2_518 0.56 -3.1
589,055 + Pf6N2E2_518 0.56 -0.1
589,055 + Pf6N2E2_518 0.56 +0.9
589,056 - Pf6N2E2_518 0.56 -0.1
589,056 - Pf6N2E2_518 0.56 -0.7
589,056 - Pf6N2E2_518 0.56 +0.9
589,188 - Pf6N2E2_518 0.69 +0.9
589,329 - Pf6N2E2_518 0.83 -0.1
589,329 - Pf6N2E2_518 0.83 -3.5
589,529 + -1.4
589,529 + -0.1
589,529 + -0.1
589,529 + +0.9
589,530 - +0.9
589,530 - -2.0
589,530 - -1.7
589,618 + +0.9
589,618 + -1.9
589,619 - -0.4
589,619 - -0.7
589,998 - Pf6N2E2_519 0.43 +0.9
590,121 - Pf6N2E2_519 0.55 -1.4
590,261 + Pf6N2E2_519 0.68 -0.7
590,262 - Pf6N2E2_519 0.68 -2.6
590,262 - Pf6N2E2_519 0.68 +0.9
590,262 - Pf6N2E2_519 0.68 +0.9
590,429 + Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 -0.1
590,430 - Pf6N2E2_519 0.84 +0.9
590,430 - Pf6N2E2_519 0.84 -0.1
590,430 - Pf6N2E2_519 0.84 +0.3
590,430 - Pf6N2E2_519 0.84 +0.9

Or see this region's nucleotide sequence