Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_515 and Pf6N2E2_516 are separated by 35 nucleotides Pf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotides
Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) <small>(from data)</small>, at 584,020 to 585,522
_515
Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379
_516
Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) <small>(from data)</small>, at 586,528 to 588,459
_517
Position (kb) 585
586
587 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.722 kb on + strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 584.723 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.082 kb on - strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.163 kb on + strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.164 kb on - strand, within Pf6N2E2_515 at 585.330 kb on - strand, within Pf6N2E2_515 at 585.415 kb on + strand at 585.440 kb on - strand at 585.448 kb on + strand at 585.520 kb on + strand at 585.521 kb on - strand at 585.521 kb on - strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.727 kb on + strand, within Pf6N2E2_516 at 585.728 kb on - strand, within Pf6N2E2_516 at 585.829 kb on - strand, within Pf6N2E2_516 at 585.969 kb on + strand, within Pf6N2E2_516 at 585.970 kb on - strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.064 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.441 kb on - strand at 586.441 kb on - strand at 586.585 kb on + strand at 586.585 kb on + strand at 586.586 kb on - strand at 586.780 kb on + strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.868 kb on + strand, within Pf6N2E2_517 at 586.869 kb on - strand at 586.869 kb on - strand, within Pf6N2E2_517 at 586.869 kb on - strand, within Pf6N2E2_517 at 586.885 kb on + strand, within Pf6N2E2_517 at 586.982 kb on - strand, within Pf6N2E2_517 at 587.111 kb on - strand, within Pf6N2E2_517 at 587.126 kb on - strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 584,722 + Pf6N2E2_515 0.47 +0.9 584,722 + Pf6N2E2_515 0.47 -3.7 584,722 + Pf6N2E2_515 0.47 +0.9 584,722 + Pf6N2E2_515 0.47 -3.3 584,723 - Pf6N2E2_515 0.47 +0.9 584,723 - Pf6N2E2_515 0.47 -2.6 585,082 - Pf6N2E2_515 0.71 -1.3 585,082 - Pf6N2E2_515 0.71 -2.6 585,163 + Pf6N2E2_515 0.76 -2.6 585,163 + Pf6N2E2_515 0.76 -1.1 585,164 - Pf6N2E2_515 0.76 -2.7 585,164 - Pf6N2E2_515 0.76 -0.1 585,330 - Pf6N2E2_515 0.87 -3.0 585,415 + +0.9 585,440 - -0.7 585,448 + -2.3 585,520 + -0.1 585,521 - +0.9 585,521 - +0.9 585,553 + +0.9 585,553 + -0.7 585,553 + -3.4 585,554 - +0.9 585,554 - +0.9 585,554 - -0.7 585,554 - -2.2 585,554 - -1.1 585,554 - +0.9 585,554 - -0.7 585,554 - -2.7 585,727 + Pf6N2E2_516 0.21 -1.1 585,728 - Pf6N2E2_516 0.21 -0.1 585,829 - Pf6N2E2_516 0.33 +0.9 585,969 + Pf6N2E2_516 0.50 +0.9 585,970 - Pf6N2E2_516 0.50 -0.1 586,063 + Pf6N2E2_516 0.61 +0.9 586,063 + Pf6N2E2_516 0.61 -0.1 586,063 + Pf6N2E2_516 0.61 -2.9 586,064 - Pf6N2E2_516 0.62 +0.9 586,279 - Pf6N2E2_516 0.88 +0.9 586,279 - Pf6N2E2_516 0.88 -3.5 586,279 - Pf6N2E2_516 0.88 -0.1 586,441 - +0.9 586,441 - +0.9 586,585 + +0.9 586,585 + +0.9 586,586 - -0.1 586,780 + Pf6N2E2_517 0.13 -0.1 586,781 - Pf6N2E2_517 0.13 +0.9 586,781 - Pf6N2E2_517 0.13 +0.9 586,868 + Pf6N2E2_517 0.18 +0.9 586,869 - +0.9 586,869 - Pf6N2E2_517 0.18 -2.6 586,869 - Pf6N2E2_517 0.18 +0.9 586,885 + Pf6N2E2_517 0.18 +0.9 586,982 - Pf6N2E2_517 0.23 -1.1 587,111 - Pf6N2E2_517 0.30 -1.6 587,126 - Pf6N2E2_517 0.31 +0.9 587,183 + Pf6N2E2_517 0.34 -2.8 587,183 + Pf6N2E2_517 0.34 +0.9
Or see this region's nucleotide sequence