Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_515

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_513 and Pf6N2E2_514 overlap by 4 nucleotidesPf6N2E2_514 and Pf6N2E2_515 are separated by 30 nucleotidesPf6N2E2_515 and Pf6N2E2_516 are separated by 35 nucleotides Pf6N2E2_513 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data), at 582,290 to 583,183 _513 Pf6N2E2_514 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data), at 583,180 to 583,989 _514 Pf6N2E2_515 - malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (from data), at 584,020 to 585,522 _515 Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379 _516 Position (kb) 584 585 586Strain fitness (log2 ratio) -3 -2 -1 0 1at 583.064 kb on + strand, within Pf6N2E2_513at 583.107 kb on + strandat 583.108 kb on - strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.118 kb on + strandat 583.119 kb on - strandat 583.119 kb on - strandat 583.140 kb on - strandat 583.249 kb on + strandat 583.249 kb on + strandat 583.249 kb on + strandat 583.250 kb on - strandat 583.250 kb on - strandat 583.435 kb on + strand, within Pf6N2E2_514at 583.436 kb on - strand, within Pf6N2E2_514at 583.490 kb on + strand, within Pf6N2E2_514at 583.491 kb on - strand, within Pf6N2E2_514at 583.534 kb on + strand, within Pf6N2E2_514at 583.535 kb on - strand, within Pf6N2E2_514at 583.535 kb on - strand, within Pf6N2E2_514at 583.604 kb on - strand, within Pf6N2E2_514at 583.750 kb on + strand, within Pf6N2E2_514at 583.750 kb on + strandat 583.751 kb on - strand, within Pf6N2E2_514at 583.751 kb on - strand, within Pf6N2E2_514at 583.798 kb on + strand, within Pf6N2E2_514at 583.798 kb on + strand, within Pf6N2E2_514at 583.799 kb on - strand, within Pf6N2E2_514at 583.799 kb on - strand, within Pf6N2E2_514at 583.906 kb on + strand, within Pf6N2E2_514at 583.907 kb on - strand, within Pf6N2E2_514at 583.907 kb on - strand, within Pf6N2E2_514at 583.909 kb on + strandat 584.003 kb on + strandat 584.047 kb on + strandat 584.047 kb on + strandat 584.048 kb on - strandat 584.048 kb on - strandat 584.048 kb on - strandat 584.722 kb on + strand, within Pf6N2E2_515at 584.722 kb on + strand, within Pf6N2E2_515at 584.722 kb on + strand, within Pf6N2E2_515at 584.722 kb on + strand, within Pf6N2E2_515at 584.723 kb on - strand, within Pf6N2E2_515at 584.723 kb on - strand, within Pf6N2E2_515at 585.082 kb on - strand, within Pf6N2E2_515at 585.082 kb on - strand, within Pf6N2E2_515at 585.163 kb on + strand, within Pf6N2E2_515at 585.163 kb on + strand, within Pf6N2E2_515at 585.164 kb on - strand, within Pf6N2E2_515at 585.164 kb on - strand, within Pf6N2E2_515at 585.330 kb on - strand, within Pf6N2E2_515at 585.415 kb on + strandat 585.440 kb on - strandat 585.448 kb on + strandat 585.520 kb on + strandat 585.521 kb on - strandat 585.521 kb on - strandat 585.553 kb on + strandat 585.553 kb on + strandat 585.553 kb on + strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.727 kb on + strand, within Pf6N2E2_516at 585.728 kb on - strand, within Pf6N2E2_516at 585.829 kb on - strand, within Pf6N2E2_516at 585.969 kb on + strand, within Pf6N2E2_516at 585.970 kb on - strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.064 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.441 kb on - strandat 586.441 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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583,064 + Pf6N2E2_513 0.87 -0.7
583,107 + +0.9
583,108 - +0.5
583,118 + +0.9
583,118 + -3.0
583,118 + -0.1
583,118 + +0.9
583,119 - -0.1
583,119 - +0.9
583,140 - -2.7
583,249 + +0.9
583,249 + +0.9
583,249 + -1.6
583,250 - +0.9
583,250 - -1.4
583,435 + Pf6N2E2_514 0.31 -2.2
583,436 - Pf6N2E2_514 0.32 -3.1
583,490 + Pf6N2E2_514 0.38 +0.9
583,491 - Pf6N2E2_514 0.38 +0.9
583,534 + Pf6N2E2_514 0.44 -0.9
583,535 - Pf6N2E2_514 0.44 +0.9
583,535 - Pf6N2E2_514 0.44 +0.9
583,604 - Pf6N2E2_514 0.52 -1.4
583,750 + Pf6N2E2_514 0.70 +0.9
583,750 + +0.9
583,751 - Pf6N2E2_514 0.70 +0.9
583,751 - Pf6N2E2_514 0.70 +0.9
583,798 + Pf6N2E2_514 0.76 +0.9
583,798 + Pf6N2E2_514 0.76 -0.1
583,799 - Pf6N2E2_514 0.76 -2.3
583,799 - Pf6N2E2_514 0.76 -2.6
583,906 + Pf6N2E2_514 0.90 -1.1
583,907 - Pf6N2E2_514 0.90 +0.9
583,907 - Pf6N2E2_514 0.90 -3.7
583,909 + +0.9
584,003 + -0.2
584,047 + +0.9
584,047 + +0.9
584,048 - +0.9
584,048 - -0.1
584,048 - +0.9
584,722 + Pf6N2E2_515 0.47 +0.9
584,722 + Pf6N2E2_515 0.47 -3.7
584,722 + Pf6N2E2_515 0.47 +0.9
584,722 + Pf6N2E2_515 0.47 -3.3
584,723 - Pf6N2E2_515 0.47 +0.9
584,723 - Pf6N2E2_515 0.47 -2.6
585,082 - Pf6N2E2_515 0.71 -1.3
585,082 - Pf6N2E2_515 0.71 -2.6
585,163 + Pf6N2E2_515 0.76 -2.6
585,163 + Pf6N2E2_515 0.76 -1.1
585,164 - Pf6N2E2_515 0.76 -2.7
585,164 - Pf6N2E2_515 0.76 -0.1
585,330 - Pf6N2E2_515 0.87 -3.0
585,415 + +0.9
585,440 - -0.7
585,448 + -2.3
585,520 + -0.1
585,521 - +0.9
585,521 - +0.9
585,553 + +0.9
585,553 + -0.7
585,553 + -3.4
585,554 - +0.9
585,554 - +0.9
585,554 - -0.7
585,554 - -2.2
585,554 - -1.1
585,554 - +0.9
585,554 - -0.7
585,554 - -2.7
585,727 + Pf6N2E2_516 0.21 -1.1
585,728 - Pf6N2E2_516 0.21 -0.1
585,829 - Pf6N2E2_516 0.33 +0.9
585,969 + Pf6N2E2_516 0.50 +0.9
585,970 - Pf6N2E2_516 0.50 -0.1
586,063 + Pf6N2E2_516 0.61 +0.9
586,063 + Pf6N2E2_516 0.61 -0.1
586,063 + Pf6N2E2_516 0.61 -2.9
586,064 - Pf6N2E2_516 0.62 +0.9
586,279 - Pf6N2E2_516 0.88 +0.9
586,279 - Pf6N2E2_516 0.88 -3.5
586,279 - Pf6N2E2_516 0.88 -0.1
586,441 - +0.9
586,441 - +0.9

Or see this region's nucleotide sequence