Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4520 and Pf6N2E2_4521 are separated by 11 nucleotides Pf6N2E2_4521 and Pf6N2E2_4522 are separated by 185 nucleotides
Pf6N2E2_4520 - hypothetical protein, at 5,254,513 to 5,256,636
_4520
Pf6N2E2_4521 - hypothetical protein, at 5,256,648 to 5,257,136
_4521
Pf6N2E2_4522 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 5,257,322 to 5,258,446
_4522
Position (kb) 5256
5257
5258 Strain fitness (log2 ratio) -2
-1
0
1 at 5255.717 kb on - strand, within Pf6N2E2_4520 at 5255.750 kb on + strand, within Pf6N2E2_4520 at 5255.750 kb on + strand, within Pf6N2E2_4520 at 5255.751 kb on - strand, within Pf6N2E2_4520 at 5255.751 kb on - strand, within Pf6N2E2_4520 at 5255.751 kb on - strand, within Pf6N2E2_4520 at 5255.988 kb on + strand, within Pf6N2E2_4520 at 5255.988 kb on + strand, within Pf6N2E2_4520 at 5255.988 kb on + strand, within Pf6N2E2_4520 at 5255.988 kb on + strand, within Pf6N2E2_4520 at 5255.988 kb on + strand, within Pf6N2E2_4520 at 5256.102 kb on + strand, within Pf6N2E2_4520 at 5256.102 kb on + strand, within Pf6N2E2_4520 at 5256.102 kb on + strand, within Pf6N2E2_4520 at 5256.102 kb on + strand, within Pf6N2E2_4520 at 5256.103 kb on - strand, within Pf6N2E2_4520 at 5256.103 kb on - strand, within Pf6N2E2_4520 at 5256.103 kb on - strand, within Pf6N2E2_4520 at 5256.131 kb on + strand, within Pf6N2E2_4520 at 5256.131 kb on + strand, within Pf6N2E2_4520 at 5256.144 kb on + strand, within Pf6N2E2_4520 at 5256.145 kb on - strand, within Pf6N2E2_4520 at 5256.228 kb on - strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.319 kb on + strand, within Pf6N2E2_4520 at 5256.320 kb on - strand, within Pf6N2E2_4520 at 5256.320 kb on - strand, within Pf6N2E2_4520 at 5256.320 kb on - strand, within Pf6N2E2_4520 at 5256.330 kb on - strand, within Pf6N2E2_4520 at 5256.355 kb on + strand, within Pf6N2E2_4520 at 5256.356 kb on - strand, within Pf6N2E2_4520 at 5256.389 kb on + strand, within Pf6N2E2_4520 at 5256.390 kb on - strand, within Pf6N2E2_4520 at 5256.510 kb on - strand at 5256.619 kb on + strand at 5256.625 kb on + strand at 5256.625 kb on + strand at 5256.626 kb on - strand at 5256.626 kb on - strand at 5256.626 kb on - strand at 5256.626 kb on - strand at 5256.649 kb on + strand at 5256.649 kb on + strand at 5256.650 kb on - strand at 5256.650 kb on - strand at 5256.869 kb on - strand, within Pf6N2E2_4521 at 5256.904 kb on + strand, within Pf6N2E2_4521 at 5256.905 kb on - strand, within Pf6N2E2_4521 at 5256.905 kb on - strand, within Pf6N2E2_4521 at 5256.905 kb on - strand, within Pf6N2E2_4521 at 5256.976 kb on + strand, within Pf6N2E2_4521 at 5256.976 kb on + strand, within Pf6N2E2_4521 at 5256.980 kb on + strand, within Pf6N2E2_4521 at 5256.980 kb on + strand, within Pf6N2E2_4521 at 5256.981 kb on - strand, within Pf6N2E2_4521 at 5256.981 kb on - strand, within Pf6N2E2_4521 at 5256.981 kb on - strand, within Pf6N2E2_4521 at 5257.060 kb on + strand, within Pf6N2E2_4521 at 5257.060 kb on + strand, within Pf6N2E2_4521 at 5257.061 kb on - strand, within Pf6N2E2_4521 at 5257.120 kb on + strand at 5257.120 kb on + strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.121 kb on - strand at 5257.403 kb on + strand at 5257.499 kb on - strand, within Pf6N2E2_4522 at 5257.683 kb on - strand, within Pf6N2E2_4522 at 5257.860 kb on - strand, within Pf6N2E2_4522 at 5257.860 kb on - strand, within Pf6N2E2_4522 at 5257.907 kb on + strand, within Pf6N2E2_4522 at 5257.907 kb on + strand, within Pf6N2E2_4522 at 5257.907 kb on + strand, within Pf6N2E2_4522 at 5257.907 kb on + strand, within Pf6N2E2_4522 at 5257.908 kb on - strand, within Pf6N2E2_4522 at 5257.908 kb on - strand, within Pf6N2E2_4522 at 5257.908 kb on - strand, within Pf6N2E2_4522 at 5257.908 kb on - strand, within Pf6N2E2_4522 at 5257.908 kb on - strand, within Pf6N2E2_4522 at 5257.916 kb on + strand, within Pf6N2E2_4522 at 5257.917 kb on - strand, within Pf6N2E2_4522 at 5257.929 kb on - strand, within Pf6N2E2_4522 at 5257.964 kb on + strand, within Pf6N2E2_4522 at 5257.965 kb on - strand, within Pf6N2E2_4522 at 5257.965 kb on - strand, within Pf6N2E2_4522 at 5258.033 kb on + strand, within Pf6N2E2_4522 at 5258.033 kb on + strand, within Pf6N2E2_4522 at 5258.033 kb on + strand, within Pf6N2E2_4522 at 5258.034 kb on - strand, within Pf6N2E2_4522 at 5258.034 kb on - strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.069 kb on + strand, within Pf6N2E2_4522 at 5258.070 kb on - strand, within Pf6N2E2_4522 at 5258.070 kb on - strand, within Pf6N2E2_4522 at 5258.070 kb on - strand, within Pf6N2E2_4522 at 5258.070 kb on - strand, within Pf6N2E2_4522 at 5258.070 kb on - strand, within Pf6N2E2_4522 at 5258.121 kb on - strand, within Pf6N2E2_4522
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 5,255,717 - Pf6N2E2_4520 0.57 +1.1 5,255,750 + Pf6N2E2_4520 0.58 +0.1 5,255,750 + Pf6N2E2_4520 0.58 -1.3 5,255,751 - Pf6N2E2_4520 0.58 -0.5 5,255,751 - Pf6N2E2_4520 0.58 +0.5 5,255,751 - Pf6N2E2_4520 0.58 -0.5 5,255,988 + Pf6N2E2_4520 0.69 +1.1 5,255,988 + Pf6N2E2_4520 0.69 -0.5 5,255,988 + Pf6N2E2_4520 0.69 -0.5 5,255,988 + Pf6N2E2_4520 0.69 -0.5 5,255,988 + Pf6N2E2_4520 0.69 -1.2 5,256,102 + Pf6N2E2_4520 0.75 +0.1 5,256,102 + Pf6N2E2_4520 0.75 +1.1 5,256,102 + Pf6N2E2_4520 0.75 +0.1 5,256,102 + Pf6N2E2_4520 0.75 +1.1 5,256,103 - Pf6N2E2_4520 0.75 -0.2 5,256,103 - Pf6N2E2_4520 0.75 +0.1 5,256,103 - Pf6N2E2_4520 0.75 +0.1 5,256,131 + Pf6N2E2_4520 0.76 -2.7 5,256,131 + Pf6N2E2_4520 0.76 -1.6 5,256,144 + Pf6N2E2_4520 0.77 +1.1 5,256,145 - Pf6N2E2_4520 0.77 -0.9 5,256,228 - Pf6N2E2_4520 0.81 +0.1 5,256,319 + Pf6N2E2_4520 0.85 -1.7 5,256,319 + Pf6N2E2_4520 0.85 +1.1 5,256,319 + Pf6N2E2_4520 0.85 +1.1 5,256,319 + Pf6N2E2_4520 0.85 +1.1 5,256,319 + Pf6N2E2_4520 0.85 -1.2 5,256,319 + Pf6N2E2_4520 0.85 +1.1 5,256,319 + Pf6N2E2_4520 0.85 -1.5 5,256,320 - Pf6N2E2_4520 0.85 +1.1 5,256,320 - Pf6N2E2_4520 0.85 +1.1 5,256,320 - Pf6N2E2_4520 0.85 -0.5 5,256,330 - Pf6N2E2_4520 0.86 +0.1 5,256,355 + Pf6N2E2_4520 0.87 +0.1 5,256,356 - Pf6N2E2_4520 0.87 +1.1 5,256,389 + Pf6N2E2_4520 0.88 +0.1 5,256,390 - Pf6N2E2_4520 0.88 -0.5 5,256,510 - +0.1 5,256,619 + -1.5 5,256,625 + -1.9 5,256,625 + -0.3 5,256,626 - +1.1 5,256,626 - +1.1 5,256,626 - +1.1 5,256,626 - -1.4 5,256,649 + +0.1 5,256,649 + +1.1 5,256,650 - -1.9 5,256,650 - +1.1 5,256,869 - Pf6N2E2_4521 0.45 +0.1 5,256,904 + Pf6N2E2_4521 0.52 -2.2 5,256,905 - Pf6N2E2_4521 0.53 -0.5 5,256,905 - Pf6N2E2_4521 0.53 -0.2 5,256,905 - Pf6N2E2_4521 0.53 +1.1 5,256,976 + Pf6N2E2_4521 0.67 +1.1 5,256,976 + Pf6N2E2_4521 0.67 -0.5 5,256,980 + Pf6N2E2_4521 0.68 +0.5 5,256,980 + Pf6N2E2_4521 0.68 -1.0 5,256,981 - Pf6N2E2_4521 0.68 +1.1 5,256,981 - Pf6N2E2_4521 0.68 +1.1 5,256,981 - Pf6N2E2_4521 0.68 +1.1 5,257,060 + Pf6N2E2_4521 0.84 +0.1 5,257,060 + Pf6N2E2_4521 0.84 +0.1 5,257,061 - Pf6N2E2_4521 0.84 +1.1 5,257,120 + -1.9 5,257,120 + +1.1 5,257,121 - -1.6 5,257,121 - -1.9 5,257,121 - -0.3 5,257,121 - +1.1 5,257,121 - +0.1 5,257,121 - +1.1 5,257,121 - +1.1 5,257,403 + -1.7 5,257,499 - Pf6N2E2_4522 0.16 -1.4 5,257,683 - Pf6N2E2_4522 0.32 +1.1 5,257,860 - Pf6N2E2_4522 0.48 +0.1 5,257,860 - Pf6N2E2_4522 0.48 +0.1 5,257,907 + Pf6N2E2_4522 0.52 +1.1 5,257,907 + Pf6N2E2_4522 0.52 +1.1 5,257,907 + Pf6N2E2_4522 0.52 -1.2 5,257,907 + Pf6N2E2_4522 0.52 +0.1 5,257,908 - Pf6N2E2_4522 0.52 -1.7 5,257,908 - Pf6N2E2_4522 0.52 +0.1 5,257,908 - Pf6N2E2_4522 0.52 +0.1 5,257,908 - Pf6N2E2_4522 0.52 -1.5 5,257,908 - Pf6N2E2_4522 0.52 +0.1 5,257,916 + Pf6N2E2_4522 0.53 -2.0 5,257,917 - Pf6N2E2_4522 0.53 -0.9 5,257,929 - Pf6N2E2_4522 0.54 +1.1 5,257,964 + Pf6N2E2_4522 0.57 -1.5 5,257,965 - Pf6N2E2_4522 0.57 -1.3 5,257,965 - Pf6N2E2_4522 0.57 +0.1 5,258,033 + Pf6N2E2_4522 0.63 -2.2 5,258,033 + Pf6N2E2_4522 0.63 -0.9 5,258,033 + Pf6N2E2_4522 0.63 -2.0 5,258,034 - Pf6N2E2_4522 0.63 +0.1 5,258,034 - Pf6N2E2_4522 0.63 +0.1 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 -0.5 5,258,069 + Pf6N2E2_4522 0.66 -0.5 5,258,069 + Pf6N2E2_4522 0.66 +1.1 5,258,069 + Pf6N2E2_4522 0.66 -2.2 5,258,070 - Pf6N2E2_4522 0.66 +0.1 5,258,070 - Pf6N2E2_4522 0.66 -0.5 5,258,070 - Pf6N2E2_4522 0.66 -0.9 5,258,070 - Pf6N2E2_4522 0.66 -1.7 5,258,070 - Pf6N2E2_4522 0.66 -0.5 5,258,121 - Pf6N2E2_4522 0.71 +0.1
Or see this region's nucleotide sequence