Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1877

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1876 and Pf6N2E2_1877 are separated by 23 nucleotides Pf6N2E2_1876 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,224,414 to 2,225,805 _1876 Pf6N2E2_1877 - putative transmembrane efflux protein., at 2,225,829 to 2,227,007 _1877 Position (kb) 2225 2226 2227 2228Strain fitness (log2 ratio) -2 -1 0 1at 2225.113 kb on - strand, within Pf6N2E2_1876at 2225.230 kb on - strand, within Pf6N2E2_1876at 2225.372 kb on - strand, within Pf6N2E2_1876at 2225.542 kb on - strand, within Pf6N2E2_1876at 2225.868 kb on - strandat 2225.870 kb on - strandat 2225.953 kb on - strand, within Pf6N2E2_1877at 2226.071 kb on - strand, within Pf6N2E2_1877at 2226.534 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.654 kb on + strand, within Pf6N2E2_1877at 2226.655 kb on - strand, within Pf6N2E2_1877at 2227.200 kb on + strandat 2227.200 kb on + strandat 2227.225 kb on - strandat 2227.361 kb on + strandat 2227.379 kb on - strandat 2227.379 kb on - strandat 2227.453 kb on + strandat 2227.458 kb on + strandat 2227.458 kb on + strandat 2227.517 kb on - strandat 2227.517 kb on - strandat 2227.643 kb on + strandat 2227.644 kb on - strandat 2227.644 kb on - strandat 2227.867 kb on + strandat 2227.900 kb on + strandat 2227.924 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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2,225,113 - Pf6N2E2_1876 0.50 +0.8
2,225,230 - Pf6N2E2_1876 0.59 +0.8
2,225,372 - Pf6N2E2_1876 0.69 -1.8
2,225,542 - Pf6N2E2_1876 0.81 +0.8
2,225,868 - -2.5
2,225,870 - -0.2
2,225,953 - Pf6N2E2_1877 0.11 -2.4
2,226,071 - Pf6N2E2_1877 0.21 +0.8
2,226,534 + Pf6N2E2_1877 0.60 -0.8
2,226,654 + Pf6N2E2_1877 0.70 -2.5
2,226,654 + Pf6N2E2_1877 0.70 -1.2
2,226,654 + Pf6N2E2_1877 0.70 -0.8
2,226,654 + Pf6N2E2_1877 0.70 +0.8
2,226,655 - Pf6N2E2_1877 0.70 +0.8
2,227,200 + +0.8
2,227,200 + -1.2
2,227,225 - +0.8
2,227,361 + +0.8
2,227,379 - -0.2
2,227,379 - +0.8
2,227,453 + -0.8
2,227,458 + -0.8
2,227,458 + -0.2
2,227,517 - +0.8
2,227,517 - +0.8
2,227,643 + +0.8
2,227,644 - +0.8
2,227,644 - +0.8
2,227,867 + +0.8
2,227,900 + -0.2
2,227,924 + +0.8

Or see this region's nucleotide sequence