Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1339

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1338 and Pf6N2E2_1339 are separated by 181 nucleotidesPf6N2E2_1339 and Pf6N2E2_1340 overlap by 23 nucleotidesPf6N2E2_1340 and Pf6N2E2_1341 are separated by 10 nucleotides Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016 _1338 Pf6N2E2_1339 - Acetylornithine deacetylase (EC 3.5.1.16), at 1,575,198 to 1,576,382 _1339 Pf6N2E2_1340 - major pilin protein fimA, at 1,576,360 to 1,577,034 _1340 Pf6N2E2_1341 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 1,577,045 to 1,577,431 _1341 Position (kb) 1575 1576 1577Strain fitness (log2 ratio) -3 -2 -1 0 1at 1574.469 kb on + strand, within Pf6N2E2_1338at 1574.469 kb on + strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.470 kb on - strand, within Pf6N2E2_1338at 1574.520 kb on + strand, within Pf6N2E2_1338at 1574.521 kb on - strand, within Pf6N2E2_1338at 1574.521 kb on - strand, within Pf6N2E2_1338at 1574.619 kb on + strand, within Pf6N2E2_1338at 1574.620 kb on - strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.643 kb on + strand, within Pf6N2E2_1338at 1574.644 kb on - strand, within Pf6N2E2_1338at 1574.644 kb on - strand, within Pf6N2E2_1338at 1574.682 kb on + strand, within Pf6N2E2_1338at 1574.682 kb on + strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.683 kb on - strand, within Pf6N2E2_1338at 1574.835 kb on + strand, within Pf6N2E2_1338at 1574.835 kb on + strand, within Pf6N2E2_1338at 1574.836 kb on - strand, within Pf6N2E2_1338at 1574.887 kb on - strand, within Pf6N2E2_1338at 1574.993 kb on - strandat 1575.082 kb on - strandat 1575.082 kb on - strandat 1575.085 kb on + strandat 1575.102 kb on - strandat 1575.223 kb on + strandat 1575.223 kb on + strandat 1575.224 kb on - strandat 1575.224 kb on - strandat 1575.224 kb on - strandat 1575.280 kb on + strandat 1575.280 kb on + strandat 1575.281 kb on - strandat 1575.539 kb on - strand, within Pf6N2E2_1339at 1576.231 kb on + strand, within Pf6N2E2_1339at 1576.231 kb on + strand, within Pf6N2E2_1339at 1576.267 kb on + strandat 1576.268 kb on - strandat 1576.268 kb on - strandat 1576.899 kb on + strand, within Pf6N2E2_1340at 1576.900 kb on - strand, within Pf6N2E2_1340at 1576.990 kb on + strandat 1577.199 kb on + strand, within Pf6N2E2_1341at 1577.199 kb on + strand, within Pf6N2E2_1341at 1577.199 kb on + strand, within Pf6N2E2_1341at 1577.199 kb on + strand, within Pf6N2E2_1341at 1577.200 kb on - strand, within Pf6N2E2_1341at 1577.200 kb on - strand, within Pf6N2E2_1341at 1577.200 kb on - strand, within Pf6N2E2_1341at 1577.349 kb on + strandat 1577.349 kb on + strand, within Pf6N2E2_1341at 1577.350 kb on - strand, within Pf6N2E2_1341at 1577.350 kb on - strand, within Pf6N2E2_1341

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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1,574,469 + Pf6N2E2_1338 0.47 +0.8
1,574,469 + Pf6N2E2_1338 0.47 -0.8
1,574,470 - Pf6N2E2_1338 0.47 +0.8
1,574,470 - Pf6N2E2_1338 0.47 -0.9
1,574,470 - Pf6N2E2_1338 0.47 +0.8
1,574,470 - Pf6N2E2_1338 0.47 -2.0
1,574,520 + Pf6N2E2_1338 0.52 -0.2
1,574,521 - Pf6N2E2_1338 0.52 +0.8
1,574,521 - Pf6N2E2_1338 0.52 -3.0
1,574,619 + Pf6N2E2_1338 0.62 +0.8
1,574,620 - Pf6N2E2_1338 0.62 +0.8
1,574,643 + Pf6N2E2_1338 0.64 +0.8
1,574,643 + Pf6N2E2_1338 0.64 +0.8
1,574,643 + Pf6N2E2_1338 0.64 -1.2
1,574,643 + Pf6N2E2_1338 0.64 -0.2
1,574,644 - Pf6N2E2_1338 0.64 -0.8
1,574,644 - Pf6N2E2_1338 0.64 +0.8
1,574,682 + Pf6N2E2_1338 0.68 -1.3
1,574,682 + Pf6N2E2_1338 0.68 +0.8
1,574,683 - Pf6N2E2_1338 0.68 -0.2
1,574,683 - Pf6N2E2_1338 0.68 +0.8
1,574,683 - Pf6N2E2_1338 0.68 -1.5
1,574,683 - Pf6N2E2_1338 0.68 -0.2
1,574,683 - Pf6N2E2_1338 0.68 +0.8
1,574,835 + Pf6N2E2_1338 0.83 +0.8
1,574,835 + Pf6N2E2_1338 0.83 +0.8
1,574,836 - Pf6N2E2_1338 0.83 -1.2
1,574,887 - Pf6N2E2_1338 0.88 +0.8
1,574,993 - -0.2
1,575,082 - +0.8
1,575,082 - -0.8
1,575,085 + +0.8
1,575,102 - -0.2
1,575,223 + +0.8
1,575,223 + +0.8
1,575,224 - -1.5
1,575,224 - -0.2
1,575,224 - -1.1
1,575,280 + +0.8
1,575,280 + -0.2
1,575,281 - -0.2
1,575,539 - Pf6N2E2_1339 0.29 -2.1
1,576,231 + Pf6N2E2_1339 0.87 -1.5
1,576,231 + Pf6N2E2_1339 0.87 -0.5
1,576,267 + -0.2
1,576,268 - -2.5
1,576,268 - +0.8
1,576,899 + Pf6N2E2_1340 0.80 -1.3
1,576,900 - Pf6N2E2_1340 0.80 +0.8
1,576,990 + +0.8
1,577,199 + Pf6N2E2_1341 0.40 +0.8
1,577,199 + Pf6N2E2_1341 0.40 +0.8
1,577,199 + Pf6N2E2_1341 0.40 -0.2
1,577,199 + Pf6N2E2_1341 0.40 -0.2
1,577,200 - Pf6N2E2_1341 0.40 -0.2
1,577,200 - Pf6N2E2_1341 0.40 -0.7
1,577,200 - Pf6N2E2_1341 0.40 +0.8
1,577,349 + +0.8
1,577,349 + Pf6N2E2_1341 0.79 -2.0
1,577,350 - Pf6N2E2_1341 0.79 -0.8
1,577,350 - Pf6N2E2_1341 0.79 +0.8

Or see this region's nucleotide sequence