Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1122

Experiment: Ying_sugar7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1121 and Pf6N2E2_1122 are separated by 64 nucleotidesPf6N2E2_1122 and Pf6N2E2_1123 are separated by 13 nucleotidesPf6N2E2_1123 and Pf6N2E2_1124 overlap by 4 nucleotides Pf6N2E2_1121 - 3-phytase precursor (EC 3.1.3.8), at 1,316,791 to 1,318,734 _1121 Pf6N2E2_1122 - MotA/TolQ/ExbB proton channel family protein, at 1,318,799 to 1,319,506 _1122 Pf6N2E2_1123 - Tol biopolymer transport system, TolR protein, at 1,319,520 to 1,319,966 _1123 Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778 _1124 Position (kb) 1318 1319 1320Strain fitness (log2 ratio) -3 -2 -1 0 1at 1317.913 kb on + strand, within Pf6N2E2_1121at 1317.914 kb on - strand, within Pf6N2E2_1121at 1317.914 kb on - strand, within Pf6N2E2_1121at 1318.162 kb on + strand, within Pf6N2E2_1121at 1318.162 kb on + strand, within Pf6N2E2_1121at 1318.162 kb on + strand, within Pf6N2E2_1121at 1318.163 kb on - strand, within Pf6N2E2_1121at 1318.163 kb on - strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.328 kb on + strand, within Pf6N2E2_1121at 1318.329 kb on - strand, within Pf6N2E2_1121at 1318.329 kb on - strand, within Pf6N2E2_1121at 1318.432 kb on + strand, within Pf6N2E2_1121at 1318.432 kb on + strand, within Pf6N2E2_1121at 1318.432 kb on + strand, within Pf6N2E2_1121at 1318.453 kb on + strand, within Pf6N2E2_1121at 1318.486 kb on + strand, within Pf6N2E2_1121at 1318.486 kb on + strand, within Pf6N2E2_1121at 1318.514 kb on - strand, within Pf6N2E2_1121at 1318.514 kb on - strand, within Pf6N2E2_1121at 1318.775 kb on - strandat 1318.775 kb on - strandat 1318.907 kb on + strand, within Pf6N2E2_1122at 1318.908 kb on - strand, within Pf6N2E2_1122at 1319.006 kb on + strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.223 kb on - strandat 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.737 kb on - strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.856 kb on + strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1320.207 kb on + strand, within Pf6N2E2_1124

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2
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1,317,913 + Pf6N2E2_1121 0.58 +0.7
1,317,914 - Pf6N2E2_1121 0.58 -2.5
1,317,914 - Pf6N2E2_1121 0.58 -1.4
1,318,162 + Pf6N2E2_1121 0.71 +0.7
1,318,162 + Pf6N2E2_1121 0.71 +0.7
1,318,162 + Pf6N2E2_1121 0.71 -1.5
1,318,163 - Pf6N2E2_1121 0.71 -0.3
1,318,163 - Pf6N2E2_1121 0.71 +0.7
1,318,328 + Pf6N2E2_1121 0.79 -0.3
1,318,328 + Pf6N2E2_1121 0.79 +0.7
1,318,328 + Pf6N2E2_1121 0.79 -2.1
1,318,328 + Pf6N2E2_1121 0.79 -0.7
1,318,328 + Pf6N2E2_1121 0.79 -0.3
1,318,328 + Pf6N2E2_1121 0.79 -1.8
1,318,329 - Pf6N2E2_1121 0.79 +0.7
1,318,329 - Pf6N2E2_1121 0.79 -0.3
1,318,432 + Pf6N2E2_1121 0.84 -0.3
1,318,432 + Pf6N2E2_1121 0.84 -0.8
1,318,432 + Pf6N2E2_1121 0.84 +0.7
1,318,453 + Pf6N2E2_1121 0.85 +0.7
1,318,486 + Pf6N2E2_1121 0.87 -0.3
1,318,486 + Pf6N2E2_1121 0.87 +0.7
1,318,514 - Pf6N2E2_1121 0.89 -2.1
1,318,514 - Pf6N2E2_1121 0.89 +0.7
1,318,775 - -0.3
1,318,775 - +0.7
1,318,907 + Pf6N2E2_1122 0.15 +0.7
1,318,908 - Pf6N2E2_1122 0.15 -1.3
1,319,006 + Pf6N2E2_1122 0.29 +0.7
1,319,170 - Pf6N2E2_1122 0.52 -3.1
1,319,170 - Pf6N2E2_1122 0.52 +0.7
1,319,170 - Pf6N2E2_1122 0.52 -0.3
1,319,170 - Pf6N2E2_1122 0.52 -1.6
1,319,223 - -0.7
1,319,381 + Pf6N2E2_1122 0.82 -0.3
1,319,381 + Pf6N2E2_1122 0.82 -0.3
1,319,427 - Pf6N2E2_1122 0.89 -3.1
1,319,427 - Pf6N2E2_1122 0.89 -0.8
1,319,427 - Pf6N2E2_1122 0.89 +0.7
1,319,577 + Pf6N2E2_1123 0.13 +0.7
1,319,577 + Pf6N2E2_1123 0.13 +0.7
1,319,737 - Pf6N2E2_1123 0.49 +0.7
1,319,739 + Pf6N2E2_1123 0.49 +0.7
1,319,739 + Pf6N2E2_1123 0.49 -0.3
1,319,740 - Pf6N2E2_1123 0.49 -0.3
1,319,740 - Pf6N2E2_1123 0.49 -0.5
1,319,740 - Pf6N2E2_1123 0.49 -0.3
1,319,856 + Pf6N2E2_1123 0.75 +0.7
1,319,857 - Pf6N2E2_1123 0.75 +1.7
1,319,857 - Pf6N2E2_1123 0.75 +0.7
1,320,207 + Pf6N2E2_1124 0.30 +0.7

Or see this region's nucleotide sequence