Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1075 and Pf6N2E2_1076 overlap by 4 nucleotides Pf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotides
Pf6N2E2_1075 - Malto-oligosyltrehalose synthase (EC 5.4.99.15), at 1,254,640 to 1,257,426
_1075
Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501
_1076
Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258
_1077
Position (kb)
1257
1258
1259
1260 Strain fitness (log2 ratio)
-2
-1
0
1 at 1256.522 kb on - strand, within Pf6N2E2_1075 at 1256.681 kb on + strand, within Pf6N2E2_1075 at 1256.787 kb on - strand, within Pf6N2E2_1075 at 1256.921 kb on + strand, within Pf6N2E2_1075 at 1256.922 kb on - strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.068 kb on + strand, within Pf6N2E2_1075 at 1257.169 kb on - strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.302 kb on + strand at 1257.303 kb on - strand at 1257.465 kb on + strand at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.072 kb on + strand, within Pf6N2E2_1076 at 1258.073 kb on - strand, within Pf6N2E2_1076 at 1258.457 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1258.874 kb on - strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.023 kb on + strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.024 kb on - strand, within Pf6N2E2_1076 at 1259.087 kb on - strand, within Pf6N2E2_1076 at 1259.251 kb on + strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1259.252 kb on - strand, within Pf6N2E2_1076 at 1260.005 kb on - strand, within Pf6N2E2_1077 at 1260.071 kb on + strand, within Pf6N2E2_1077 at 1260.307 kb on - strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.315 kb on + strand, within Pf6N2E2_1077 at 1260.318 kb on + strand, within Pf6N2E2_1077 at 1260.319 kb on - strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.387 kb on + strand, within Pf6N2E2_1077 at 1260.388 kb on - strand, within Pf6N2E2_1077
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 1,256,522 - Pf6N2E2_1075 0.68 +0.2 1,256,681 + Pf6N2E2_1075 0.73 -1.5 1,256,787 - Pf6N2E2_1075 0.77 +0.8 1,256,921 + Pf6N2E2_1075 0.82 +0.8 1,256,922 - Pf6N2E2_1075 0.82 +0.8 1,257,068 + Pf6N2E2_1075 0.87 -0.8 1,257,068 + Pf6N2E2_1075 0.87 +0.8 1,257,169 - +0.8 1,257,302 + +0.8 1,257,302 + -0.5 1,257,302 + -0.8 1,257,302 + +0.8 1,257,303 - +0.8 1,257,465 + +0.8 1,258,072 + Pf6N2E2_1076 0.31 -2.1 1,258,072 + Pf6N2E2_1076 0.31 +0.8 1,258,072 + Pf6N2E2_1076 0.31 +0.8 1,258,073 - Pf6N2E2_1076 0.31 +0.8 1,258,457 - Pf6N2E2_1076 0.50 -0.2 1,258,874 - Pf6N2E2_1076 0.70 -2.0 1,258,874 - Pf6N2E2_1076 0.70 +0.8 1,259,023 + Pf6N2E2_1076 0.77 +0.8 1,259,023 + Pf6N2E2_1076 0.77 -2.0 1,259,024 - Pf6N2E2_1076 0.77 +0.8 1,259,024 - Pf6N2E2_1076 0.77 -1.2 1,259,024 - Pf6N2E2_1076 0.77 +0.8 1,259,087 - Pf6N2E2_1076 0.80 +0.8 1,259,251 + Pf6N2E2_1076 0.88 +0.8 1,259,252 - Pf6N2E2_1076 0.88 -0.5 1,259,252 - Pf6N2E2_1076 0.88 +0.8 1,260,005 - Pf6N2E2_1077 0.29 +0.8 1,260,071 + Pf6N2E2_1077 0.33 -0.2 1,260,307 - Pf6N2E2_1077 0.46 -2.2 1,260,315 + Pf6N2E2_1077 0.46 +0.8 1,260,315 + Pf6N2E2_1077 0.46 -0.2 1,260,318 + Pf6N2E2_1077 0.47 -1.3 1,260,319 - Pf6N2E2_1077 0.47 +0.8 1,260,387 + Pf6N2E2_1077 0.50 -1.7 1,260,387 + Pf6N2E2_1077 0.50 +0.8 1,260,388 - Pf6N2E2_1077 0.51 +0.8
Or see this region's nucleotide sequence