Experiment: Ying_Others16 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_303 and Pf6N2E2_304 are separated by 88 nucleotides Pf6N2E2_304 and Pf6N2E2_305 overlap by 4 nucleotides Pf6N2E2_305 and Pf6N2E2_306 overlap by 4 nucleotides Pf6N2E2_306 and Pf6N2E2_307 overlap by 1 nucleotides Pf6N2E2_307 and Pf6N2E2_308 overlap by 1 nucleotides
Pf6N2E2_303 - Glutathione S-transferase, omega (EC 2.5.1.18), at 373,917 to 374,915
_303
Pf6N2E2_304 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 375,004 to 376,005
_304
Pf6N2E2_305 - tRNA 2-thiouridine synthesizing protein E (EC 2.8.1.-), at 376,002 to 376,337
_305
Pf6N2E2_306 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 376,334 to 376,627
_306
Pf6N2E2_307 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 376,627 to 376,989
_307
Pf6N2E2_308 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 376,989 to 377,381
_308
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.035 kb on + strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.036 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.039 kb on - strand, within Pf6N2E2_303 at 374.052 kb on - strand, within Pf6N2E2_303 at 374.092 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.167 kb on + strand, within Pf6N2E2_303 at 374.168 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.225 kb on - strand, within Pf6N2E2_303 at 374.239 kb on + strand, within Pf6N2E2_303 at 374.308 kb on + strand, within Pf6N2E2_303 at 374.309 kb on - strand, within Pf6N2E2_303 at 374.366 kb on - strand, within Pf6N2E2_303 at 374.545 kb on + strand, within Pf6N2E2_303 at 374.546 kb on - strand, within Pf6N2E2_303 at 374.566 kb on + strand, within Pf6N2E2_303 at 374.656 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.670 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.749 kb on + strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.750 kb on - strand, within Pf6N2E2_303 at 374.875 kb on + strand at 374.875 kb on + strand at 374.875 kb on + strand at 374.876 kb on - strand at 374.876 kb on - strand at 374.904 kb on + strand at 374.953 kb on + strand at 374.973 kb on - strand at 375.005 kb on + strand at 375.032 kb on + strand at 375.123 kb on - strand, within Pf6N2E2_304 at 375.563 kb on - strand, within Pf6N2E2_304 at 375.599 kb on + strand, within Pf6N2E2_304 at 375.684 kb on - strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.713 kb on + strand, within Pf6N2E2_304 at 375.820 kb on + strand, within Pf6N2E2_304
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1 remove 374,035 + Pf6N2E2_303 0.12 +0.9 374,035 + Pf6N2E2_303 0.12 +0.9 374,035 + Pf6N2E2_303 0.12 -0.8 374,036 - Pf6N2E2_303 0.12 +0.9 374,036 - Pf6N2E2_303 0.12 -0.0 374,036 - Pf6N2E2_303 0.12 +0.9 374,036 - Pf6N2E2_303 0.12 -0.6 374,039 - Pf6N2E2_303 0.12 -0.1 374,039 - Pf6N2E2_303 0.12 -1.8 374,039 - Pf6N2E2_303 0.12 -0.1 374,052 - Pf6N2E2_303 0.14 -0.1 374,092 + Pf6N2E2_303 0.18 -0.9 374,167 + Pf6N2E2_303 0.25 -0.8 374,167 + Pf6N2E2_303 0.25 -0.1 374,168 - Pf6N2E2_303 0.25 +0.9 374,225 - Pf6N2E2_303 0.31 -0.1 374,225 - Pf6N2E2_303 0.31 -0.1 374,239 + Pf6N2E2_303 0.32 +0.9 374,308 + Pf6N2E2_303 0.39 +0.9 374,309 - Pf6N2E2_303 0.39 +0.9 374,366 - Pf6N2E2_303 0.45 +0.9 374,545 + Pf6N2E2_303 0.63 -1.9 374,546 - Pf6N2E2_303 0.63 -1.7 374,566 + Pf6N2E2_303 0.65 +0.9 374,656 + Pf6N2E2_303 0.74 +0.9 374,670 + Pf6N2E2_303 0.75 -0.1 374,670 + Pf6N2E2_303 0.75 -1.1 374,749 + Pf6N2E2_303 0.83 -1.1 374,749 + Pf6N2E2_303 0.83 +0.9 374,749 + Pf6N2E2_303 0.83 +0.9 374,749 + Pf6N2E2_303 0.83 +0.9 374,749 + Pf6N2E2_303 0.83 -1.3 374,749 + Pf6N2E2_303 0.83 +0.9 374,749 + Pf6N2E2_303 0.83 -0.6 374,749 + Pf6N2E2_303 0.83 -0.1 374,749 + Pf6N2E2_303 0.83 +0.9 374,749 + Pf6N2E2_303 0.83 +0.9 374,750 - Pf6N2E2_303 0.83 -0.3 374,750 - Pf6N2E2_303 0.83 -1.4 374,750 - Pf6N2E2_303 0.83 +0.9 374,750 - Pf6N2E2_303 0.83 -1.0 374,750 - Pf6N2E2_303 0.83 +0.9 374,750 - Pf6N2E2_303 0.83 -0.1 374,875 + -0.7 374,875 + +0.9 374,875 + -2.2 374,876 - +0.9 374,876 - +0.9 374,904 + -0.1 374,953 + -0.1 374,973 - -0.6 375,005 + -0.7 375,032 + +0.9 375,123 - Pf6N2E2_304 0.12 -0.1 375,563 - Pf6N2E2_304 0.56 -0.7 375,599 + Pf6N2E2_304 0.59 +0.9 375,684 - Pf6N2E2_304 0.68 -0.1 375,713 + Pf6N2E2_304 0.71 -1.9 375,713 + Pf6N2E2_304 0.71 +0.9 375,820 + Pf6N2E2_304 0.81 +0.9
Or see this region's nucleotide sequence