Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1432

Experiment: Ying_Others16 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotidesPf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotidesPf6N2E2_1433 and Pf6N2E2_1434 are separated by 13 nucleotides Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765 _1431 Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658 _1432 Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508 _1433 Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682 _1434 Position (kb) 1681 1682 1683Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1681.371 kb on + strand, within Pf6N2E2_1431at 1681.372 kb on - strand, within Pf6N2E2_1431at 1681.492 kb on - strand, within Pf6N2E2_1431at 1681.779 kb on - strandat 1681.895 kb on + strand, within Pf6N2E2_1432at 1681.896 kb on - strand, within Pf6N2E2_1432at 1681.963 kb on - strand, within Pf6N2E2_1432at 1682.351 kb on + strand, within Pf6N2E2_1432at 1682.351 kb on + strand, within Pf6N2E2_1432at 1682.352 kb on - strand, within Pf6N2E2_1432at 1682.573 kb on + strandat 1682.606 kb on + strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.638 kb on + strandat 1682.638 kb on + strandat 1682.911 kb on + strand, within Pf6N2E2_1433at 1683.031 kb on - strand, within Pf6N2E2_1433at 1683.233 kb on - strand, within Pf6N2E2_1433at 1683.334 kb on - strand, within Pf6N2E2_1433at 1683.466 kb on + strandat 1683.466 kb on + strandat 1683.467 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Others16 rep A; time point 1
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1,681,371 + Pf6N2E2_1431 0.63 +0.8
1,681,372 - Pf6N2E2_1431 0.63 -0.8
1,681,492 - Pf6N2E2_1431 0.74 -0.2
1,681,779 - -0.2
1,681,895 + Pf6N2E2_1432 0.13 +0.8
1,681,896 - Pf6N2E2_1432 0.13 +0.8
1,681,963 - Pf6N2E2_1432 0.21 -0.2
1,682,351 + Pf6N2E2_1432 0.65 -0.2
1,682,351 + Pf6N2E2_1432 0.65 -2.0
1,682,352 - Pf6N2E2_1432 0.65 -1.0
1,682,573 + -1.7
1,682,606 + +0.8
1,682,607 - -0.8
1,682,607 - -0.8
1,682,607 - -2.2
1,682,607 - +0.8
1,682,607 - +2.8
1,682,607 - -0.5
1,682,638 + +0.8
1,682,638 + -0.2
1,682,911 + Pf6N2E2_1433 0.24 -0.5
1,683,031 - Pf6N2E2_1433 0.39 -2.0
1,683,233 - Pf6N2E2_1433 0.65 -0.9
1,683,334 - Pf6N2E2_1433 0.78 +0.8
1,683,466 + +0.8
1,683,466 + +0.8
1,683,467 - +0.8

Or see this region's nucleotide sequence