Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4587 and Pf6N2E2_4588 are separated by 73 nucleotides Pf6N2E2_4588 and Pf6N2E2_4589 are separated by 79 nucleotides Pf6N2E2_4589 and Pf6N2E2_4590 are separated by 54 nucleotides Pf6N2E2_4590 and Pf6N2E2_4591 are separated by 9 nucleotides Pf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotides
Pf6N2E2_4587 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 5,317,569 to 5,318,291
_4587
Pf6N2E2_4588 - COG2363, at 5,318,365 to 5,318,745
_4588
Pf6N2E2_4589 - Sulfur carrier protein ThiS, at 5,318,825 to 5,319,043
_4589
Pf6N2E2_4590 - Thiazole biosynthesis protein ThiG, at 5,319,098 to 5,319,892
_4590
Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627
_4591
Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095
_4592
Position (kb) 5319
5320 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5318.280 kb on + strand at 5318.281 kb on - strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.292 kb on - strand at 5318.327 kb on + strand at 5318.328 kb on - strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.373 kb on + strand at 5318.373 kb on + strand at 5318.374 kb on - strand at 5318.374 kb on - strand at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.468 kb on - strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.759 kb on + strand at 5318.759 kb on + strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.805 kb on + strand at 5318.805 kb on + strand at 5319.142 kb on + strand at 5319.142 kb on + strand at 5319.143 kb on - strand at 5319.180 kb on - strand, within Pf6N2E2_4590 at 5319.314 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.821 kb on + strand at 5319.852 kb on - strand at 5319.890 kb on + strand at 5319.890 kb on + strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.466 kb on + strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.626 kb on - strand at 5320.690 kb on + strand at 5320.690 kb on + strand at 5320.691 kb on - strand at 5320.691 kb on - strand at 5320.716 kb on + strand at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 5,318,280 + -0.5 5,318,281 - -1.7 5,318,291 + +1.1 5,318,291 + -0.6 5,318,291 + -0.6 5,318,291 + -1.6 5,318,291 + -1.2 5,318,292 - -0.8 5,318,327 + +1.1 5,318,328 - -1.5 5,318,329 + +1.1 5,318,329 + +1.1 5,318,329 + +1.1 5,318,329 + +0.1 5,318,329 + -0.8 5,318,329 + -1.4 5,318,329 + -0.5 5,318,329 + -0.0 5,318,329 + -0.9 5,318,329 + +1.1 5,318,329 + -0.6 5,318,329 + +1.9 5,318,329 + +1.1 5,318,330 - -0.5 5,318,330 - -1.5 5,318,330 - +0.1 5,318,330 - +1.1 5,318,330 - +1.1 5,318,330 - -0.9 5,318,330 - +0.1 5,318,330 - -1.0 5,318,330 - +0.1 5,318,330 - +1.1 5,318,373 + -0.1 5,318,373 + +0.1 5,318,374 - +1.1 5,318,374 - +0.1 5,318,467 + Pf6N2E2_4588 0.27 +0.1 5,318,467 + Pf6N2E2_4588 0.27 +0.1 5,318,468 - Pf6N2E2_4588 0.27 +1.1 5,318,497 + Pf6N2E2_4588 0.35 +1.1 5,318,497 + Pf6N2E2_4588 0.35 +1.1 5,318,497 + Pf6N2E2_4588 0.35 +1.1 5,318,497 + Pf6N2E2_4588 0.35 -0.9 5,318,497 + Pf6N2E2_4588 0.35 +1.1 5,318,498 - Pf6N2E2_4588 0.35 +0.4 5,318,498 - Pf6N2E2_4588 0.35 +1.1 5,318,498 - Pf6N2E2_4588 0.35 +1.1 5,318,498 - Pf6N2E2_4588 0.35 -2.2 5,318,498 - Pf6N2E2_4588 0.35 -0.5 5,318,512 - Pf6N2E2_4588 0.39 +0.1 5,318,512 - Pf6N2E2_4588 0.39 +1.1 5,318,603 + Pf6N2E2_4588 0.62 +0.1 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,603 + Pf6N2E2_4588 0.62 +0.1 5,318,603 + Pf6N2E2_4588 0.62 -0.9 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,603 + Pf6N2E2_4588 0.62 -0.5 5,318,603 + Pf6N2E2_4588 0.62 +2.1 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,603 + Pf6N2E2_4588 0.62 +1.1 5,318,604 - Pf6N2E2_4588 0.63 -0.2 5,318,604 - Pf6N2E2_4588 0.63 -0.6 5,318,604 - Pf6N2E2_4588 0.63 +0.1 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 +0.1 5,318,604 - Pf6N2E2_4588 0.63 -1.1 5,318,604 - Pf6N2E2_4588 0.63 -0.4 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 -0.9 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 -0.6 5,318,623 + Pf6N2E2_4588 0.68 -1.2 5,318,623 + Pf6N2E2_4588 0.68 -0.2 5,318,623 + Pf6N2E2_4588 0.68 +0.1 5,318,624 - Pf6N2E2_4588 0.68 -0.7 5,318,624 - Pf6N2E2_4588 0.68 -0.9 5,318,624 - Pf6N2E2_4588 0.68 +0.1 5,318,759 + -1.7 5,318,759 + +0.1 5,318,760 - +0.1 5,318,760 - -0.5 5,318,760 - +0.1 5,318,805 + -2.0 5,318,805 + -0.5 5,319,142 + +1.1 5,319,142 + -3.4 5,319,143 - -2.4 5,319,180 - Pf6N2E2_4590 0.10 +0.1 5,319,314 + Pf6N2E2_4590 0.27 +1.1 5,319,515 + Pf6N2E2_4590 0.52 -3.5 5,319,515 + Pf6N2E2_4590 0.52 +1.1 5,319,515 + Pf6N2E2_4590 0.52 -2.8 5,319,516 - Pf6N2E2_4590 0.53 +0.1 5,319,516 - Pf6N2E2_4590 0.53 +1.1 5,319,516 - Pf6N2E2_4590 0.53 +1.1 5,319,624 - Pf6N2E2_4590 0.66 -2.5 5,319,624 - Pf6N2E2_4590 0.66 -1.7 5,319,821 + -0.5 5,319,852 - +1.1 5,319,890 + -0.5 5,319,890 + +1.1 5,319,891 - +1.1 5,319,891 - +1.1 5,319,891 - +0.1 5,319,891 - +1.1 5,319,891 - +0.1 5,319,891 - -2.1 5,320,457 + Pf6N2E2_4591 0.76 +1.1 5,320,457 + Pf6N2E2_4591 0.76 +1.1 5,320,457 + Pf6N2E2_4591 0.76 -0.8 5,320,457 + Pf6N2E2_4591 0.76 -0.7 5,320,458 - Pf6N2E2_4591 0.77 +0.1 5,320,458 - Pf6N2E2_4591 0.77 +1.1 5,320,466 + Pf6N2E2_4591 0.78 -0.4 5,320,467 - Pf6N2E2_4591 0.78 +1.1 5,320,467 - Pf6N2E2_4591 0.78 -0.5 5,320,467 - Pf6N2E2_4591 0.78 +1.1 5,320,467 - Pf6N2E2_4591 0.78 +1.1 5,320,467 - Pf6N2E2_4591 0.78 +0.1 5,320,467 - Pf6N2E2_4591 0.78 +1.1 5,320,467 - Pf6N2E2_4591 0.78 +0.1 5,320,626 - +1.1 5,320,690 + -1.2 5,320,690 + +1.1 5,320,691 - +0.9 5,320,691 - +0.1 5,320,716 + +1.1 5,320,836 + Pf6N2E2_4592 0.20 -0.9 5,320,836 + Pf6N2E2_4592 0.20 -0.5 5,320,836 + Pf6N2E2_4592 0.20 +0.1 5,320,836 + Pf6N2E2_4592 0.20 +0.4 5,320,836 + Pf6N2E2_4592 0.20 -0.7 5,320,836 + Pf6N2E2_4592 0.20 +0.1 5,320,837 - Pf6N2E2_4592 0.20 -0.3 5,320,837 - Pf6N2E2_4592 0.20 -0.5 5,320,837 - Pf6N2E2_4592 0.20 -0.6 5,320,837 - Pf6N2E2_4592 0.20 +0.1 5,320,837 - Pf6N2E2_4592 0.20 +1.1 5,320,837 - Pf6N2E2_4592 0.20 -2.1 5,320,837 - Pf6N2E2_4592 0.20 +1.1 5,320,837 - Pf6N2E2_4592 0.20 -0.5 5,320,881 + Pf6N2E2_4592 0.34 +0.1 5,320,881 + Pf6N2E2_4592 0.34 +0.1 5,320,881 + Pf6N2E2_4592 0.34 +0.8 5,320,882 - Pf6N2E2_4592 0.34 +1.1 5,320,882 - Pf6N2E2_4592 0.34 +0.1 5,320,882 - Pf6N2E2_4592 0.34 -0.9 5,320,882 - Pf6N2E2_4592 0.34 +0.1
Or see this region's nucleotide sequence