Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_32 and Pf6N2E2_33 are separated by 179 nucleotides Pf6N2E2_33 and Pf6N2E2_34 are separated by 56 nucleotides
Pf6N2E2_32 - Membrane protein mosC, at 70,667 to 71,818
_32
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.018 kb on + strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.019 kb on - strand, within Pf6N2E2_32 at 71.073 kb on + strand, within Pf6N2E2_32 at 71.282 kb on + strand, within Pf6N2E2_32 at 71.283 kb on - strand, within Pf6N2E2_32 at 71.834 kb on + strand at 71.835 kb on - strand at 71.865 kb on + strand at 71.865 kb on + strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.866 kb on - strand at 71.959 kb on + strand at 71.959 kb on + strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.960 kb on - strand at 71.964 kb on + strand at 72.011 kb on - strand at 72.011 kb on - strand at 72.027 kb on + strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.028 kb on - strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.031 kb on + strand at 72.032 kb on - strand at 72.032 kb on - strand at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33 at 73.130 kb on - strand at 73.162 kb on + strand at 73.163 kb on - strand at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 71,018 + Pf6N2E2_32 0.30 +1.0 71,018 + Pf6N2E2_32 0.30 +1.0 71,019 - Pf6N2E2_32 0.31 -0.6 71,019 - Pf6N2E2_32 0.31 -0.0 71,019 - Pf6N2E2_32 0.31 +0.4 71,019 - Pf6N2E2_32 0.31 -1.0 71,073 + Pf6N2E2_32 0.35 +1.0 71,282 + Pf6N2E2_32 0.53 +1.0 71,283 - Pf6N2E2_32 0.53 -0.6 71,834 + +1.0 71,835 - +1.0 71,865 + +1.0 71,865 + -1.4 71,866 - -0.0 71,866 - -1.4 71,866 - -1.4 71,959 + +1.0 71,959 + -0.0 71,960 - -0.6 71,960 - -0.4 71,960 - -1.0 71,960 - +1.0 71,960 - -0.0 71,964 + -1.8 72,011 - -1.2 72,011 - -1.0 72,027 + -1.3 72,028 - -2.0 72,028 - +1.0 72,028 - +1.0 72,028 - -0.0 72,028 - -0.5 72,028 - +1.0 72,028 - +1.0 72,028 - -0.0 72,031 + -0.9 72,031 + +1.0 72,031 + -0.6 72,031 + +1.0 72,032 - +1.0 72,032 - -1.7 72,255 + Pf6N2E2_33 0.26 +0.6 72,694 + Pf6N2E2_33 0.69 +1.0 72,695 - Pf6N2E2_33 0.70 -0.6 73,130 - +1.0 73,162 + -0.0 73,163 - +1.0 73,340 + Pf6N2E2_34 0.23 +1.0 73,458 - Pf6N2E2_34 0.32 +1.0 73,549 + Pf6N2E2_34 0.40 +1.0 73,550 - Pf6N2E2_34 0.40 -0.9 73,561 + Pf6N2E2_34 0.40 +1.0 73,562 - Pf6N2E2_34 0.41 +1.0 73,756 + Pf6N2E2_34 0.56 +1.0 73,756 + Pf6N2E2_34 0.56 -1.0
Or see this region's nucleotide sequence