Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3230

Experiment: Ying_Nucleobase14 rep C; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3228 and Pf6N2E2_3229 are separated by 132 nucleotidesPf6N2E2_3229 and Pf6N2E2_3230 are separated by 10 nucleotidesPf6N2E2_3230 and Pf6N2E2_3231 are separated by 10 nucleotidesPf6N2E2_3231 and Pf6N2E2_3232 overlap by 1 nucleotides Pf6N2E2_3228 - N-acetylneuraminic acid outer membrane channel protein NanC, at 3,733,122 to 3,733,844 _3228 Pf6N2E2_3229 - Putative glutathione transporter, ATP-binding component, at 3,733,977 to 3,734,744 _3229 Pf6N2E2_3230 - Putative glutathione transporter, ATP-binding component, at 3,734,755 to 3,735,594 _3230 Pf6N2E2_3231 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 3,735,605 to 3,736,507 _3231 Pf6N2E2_3232 - Dipeptide transport system permease protein DppB (TC 3.A.1.5.2), at 3,736,507 to 3,737,544 _3232 Position (kb) 3734 3735 3736Strain fitness (log2 ratio) -2 -1 0 1at 3733.824 kb on + strandat 3733.828 kb on - strandat 3733.872 kb on + strandat 3733.873 kb on - strandat 3733.876 kb on + strandat 3733.921 kb on - strandat 3733.949 kb on - strandat 3733.957 kb on + strandat 3733.957 kb on + strandat 3733.957 kb on + strandat 3733.958 kb on - strandat 3733.963 kb on + strandat 3733.967 kb on + strandat 3733.967 kb on + strandat 3733.970 kb on + strandat 3733.970 kb on + strandat 3734.001 kb on + strandat 3734.001 kb on + strandat 3734.001 kb on + strandat 3734.001 kb on + strandat 3734.001 kb on + strandat 3734.001 kb on + strandat 3734.002 kb on - strandat 3734.002 kb on - strandat 3734.036 kb on - strandat 3734.164 kb on + strand, within Pf6N2E2_3229at 3734.164 kb on + strand, within Pf6N2E2_3229at 3734.164 kb on + strand, within Pf6N2E2_3229at 3734.507 kb on - strand, within Pf6N2E2_3229at 3734.849 kb on + strand, within Pf6N2E2_3230at 3734.850 kb on - strand, within Pf6N2E2_3230at 3734.850 kb on - strand, within Pf6N2E2_3230at 3735.152 kb on + strand, within Pf6N2E2_3230at 3735.152 kb on + strand, within Pf6N2E2_3230at 3735.153 kb on - strand, within Pf6N2E2_3230at 3735.153 kb on - strand, within Pf6N2E2_3230at 3735.174 kb on + strand, within Pf6N2E2_3230at 3735.245 kb on + strand, within Pf6N2E2_3230at 3735.245 kb on + strand, within Pf6N2E2_3230at 3735.245 kb on + strand, within Pf6N2E2_3230at 3735.246 kb on - strand, within Pf6N2E2_3230at 3735.293 kb on + strand, within Pf6N2E2_3230at 3735.293 kb on + strand, within Pf6N2E2_3230at 3735.430 kb on + strand, within Pf6N2E2_3230at 3735.571 kb on + strandat 3735.583 kb on + strandat 3735.584 kb on - strandat 3735.726 kb on + strand, within Pf6N2E2_3231at 3735.727 kb on - strand, within Pf6N2E2_3231at 3735.727 kb on - strand, within Pf6N2E2_3231at 3735.727 kb on - strand, within Pf6N2E2_3231at 3736.200 kb on + strand, within Pf6N2E2_3231at 3736.200 kb on + strand, within Pf6N2E2_3231at 3736.200 kb on + strand, within Pf6N2E2_3231at 3736.200 kb on + strand, within Pf6N2E2_3231at 3736.201 kb on - strand, within Pf6N2E2_3231at 3736.201 kb on - strand, within Pf6N2E2_3231at 3736.201 kb on - strand, within Pf6N2E2_3231at 3736.201 kb on - strand, within Pf6N2E2_3231at 3736.222 kb on - strand, within Pf6N2E2_3231at 3736.222 kb on - strand, within Pf6N2E2_3231at 3736.251 kb on + strand, within Pf6N2E2_3231at 3736.252 kb on - strand, within Pf6N2E2_3231at 3736.457 kb on - strandat 3736.535 kb on + strandat 3736.536 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1
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3,733,824 + +1.0
3,733,828 - -1.0
3,733,872 + +1.0
3,733,873 - -2.3
3,733,876 + -0.0
3,733,921 - -1.1
3,733,949 - -0.5
3,733,957 + +1.0
3,733,957 + -1.6
3,733,957 + +1.0
3,733,958 - +1.0
3,733,963 + -0.7
3,733,967 + -0.7
3,733,967 + -1.6
3,733,970 + +1.0
3,733,970 + +1.0
3,734,001 + +1.0
3,734,001 + +1.0
3,734,001 + +1.0
3,734,001 + -0.0
3,734,001 + +1.0
3,734,001 + +1.0
3,734,002 - +1.0
3,734,002 - +1.0
3,734,036 - +1.0
3,734,164 + Pf6N2E2_3229 0.24 -1.3
3,734,164 + Pf6N2E2_3229 0.24 +1.0
3,734,164 + Pf6N2E2_3229 0.24 +1.0
3,734,507 - Pf6N2E2_3229 0.69 -0.7
3,734,849 + Pf6N2E2_3230 0.11 -1.2
3,734,850 - Pf6N2E2_3230 0.11 -0.0
3,734,850 - Pf6N2E2_3230 0.11 -1.9
3,735,152 + Pf6N2E2_3230 0.47 +1.0
3,735,152 + Pf6N2E2_3230 0.47 -0.6
3,735,153 - Pf6N2E2_3230 0.47 -0.6
3,735,153 - Pf6N2E2_3230 0.47 -0.9
3,735,174 + Pf6N2E2_3230 0.50 +0.2
3,735,245 + Pf6N2E2_3230 0.58 -0.6
3,735,245 + Pf6N2E2_3230 0.58 -0.6
3,735,245 + Pf6N2E2_3230 0.58 -0.6
3,735,246 - Pf6N2E2_3230 0.58 -1.0
3,735,293 + Pf6N2E2_3230 0.64 +1.0
3,735,293 + Pf6N2E2_3230 0.64 +1.0
3,735,430 + Pf6N2E2_3230 0.80 -0.6
3,735,571 + -0.6
3,735,583 + +1.0
3,735,584 - +1.0
3,735,726 + Pf6N2E2_3231 0.13 -1.0
3,735,727 - Pf6N2E2_3231 0.14 +1.0
3,735,727 - Pf6N2E2_3231 0.14 +1.0
3,735,727 - Pf6N2E2_3231 0.14 +1.0
3,736,200 + Pf6N2E2_3231 0.66 -0.8
3,736,200 + Pf6N2E2_3231 0.66 -0.3
3,736,200 + Pf6N2E2_3231 0.66 -0.8
3,736,200 + Pf6N2E2_3231 0.66 +1.0
3,736,201 - Pf6N2E2_3231 0.66 -0.3
3,736,201 - Pf6N2E2_3231 0.66 -0.9
3,736,201 - Pf6N2E2_3231 0.66 -0.0
3,736,201 - Pf6N2E2_3231 0.66 -0.0
3,736,222 - Pf6N2E2_3231 0.68 +1.0
3,736,222 - Pf6N2E2_3231 0.68 -0.0
3,736,251 + Pf6N2E2_3231 0.72 -0.0
3,736,252 - Pf6N2E2_3231 0.72 -0.0
3,736,457 - -0.0
3,736,535 + -0.6
3,736,536 - +1.0

Or see this region's nucleotide sequence