Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1318 and Pf6N2E2_1319 are separated by 182 nucleotides Pf6N2E2_1319 and Pf6N2E2_1320 are separated by 51 nucleotides Pf6N2E2_1320 and Pf6N2E2_1321 are separated by 78 nucleotides Pf6N2E2_1321 and Pf6N2E2_1322 overlap by 8 nucleotides
Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502
_1318
Pf6N2E2_1319 - FIG074102: hypothetical protein, at 1,553,685 to 1,554,050
_1319
Pf6N2E2_1320 - Putative response regulator, at 1,554,102 to 1,554,761
_1320
Pf6N2E2_1321 - MoaF protein precursor, at 1,554,840 to 1,555,661
_1321
Pf6N2E2_1322 - MoaE protein, at 1,555,654 to 1,556,454
_1322
Position (kb)
1554
1555 Strain fitness (log2 ratio)
-1
0
1
2 at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.839 kb on - strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.952 kb on + strand, within Pf6N2E2_1319 at 1554.033 kb on + strand at 1554.033 kb on + strand at 1554.153 kb on - strand at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.271 kb on - strand, within Pf6N2E2_1320 at 1554.379 kb on - strand, within Pf6N2E2_1320 at 1554.716 kb on - strand at 1554.779 kb on - strand at 1554.838 kb on + strand at 1554.839 kb on - strand at 1554.839 kb on - strand at 1555.073 kb on - strand, within Pf6N2E2_1321 at 1555.073 kb on - strand, within Pf6N2E2_1321 at 1555.075 kb on - strand, within Pf6N2E2_1321 at 1555.075 kb on - strand, within Pf6N2E2_1321 at 1555.366 kb on - strand, within Pf6N2E2_1321 at 1555.378 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.630 kb on + strand at 1555.648 kb on - strand at 1555.669 kb on - strand at 1555.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 1,553,519 + -1.2 1,553,519 + -0.2 1,553,519 + +0.8 1,553,519 + +0.8 1,553,519 + +0.8 1,553,520 - +0.8 1,553,520 - -0.2 1,553,520 - +0.6 1,553,520 - -0.7 1,553,520 - +0.8 1,553,520 - -0.5 1,553,839 - Pf6N2E2_1319 0.42 +0.8 1,553,874 + Pf6N2E2_1319 0.52 -0.2 1,553,874 + Pf6N2E2_1319 0.52 +0.8 1,553,874 + Pf6N2E2_1319 0.52 -0.7 1,553,952 + Pf6N2E2_1319 0.73 +0.8 1,554,033 + +0.8 1,554,033 + +0.8 1,554,153 - -0.2 1,554,187 - Pf6N2E2_1320 0.13 +0.8 1,554,187 - Pf6N2E2_1320 0.13 +0.8 1,554,258 + Pf6N2E2_1320 0.24 +0.8 1,554,258 + Pf6N2E2_1320 0.24 -0.2 1,554,271 - Pf6N2E2_1320 0.26 +2.3 1,554,379 - Pf6N2E2_1320 0.42 +0.8 1,554,716 - -0.2 1,554,779 - -0.2 1,554,838 + +0.8 1,554,839 - -0.8 1,554,839 - +0.8 1,555,073 - Pf6N2E2_1321 0.28 -0.7 1,555,073 - Pf6N2E2_1321 0.28 -0.7 1,555,075 - Pf6N2E2_1321 0.29 +0.8 1,555,075 - Pf6N2E2_1321 0.29 +0.8 1,555,366 - Pf6N2E2_1321 0.64 -1.0 1,555,378 - Pf6N2E2_1321 0.65 +0.8 1,555,459 - Pf6N2E2_1321 0.75 -0.2 1,555,459 - Pf6N2E2_1321 0.75 -0.2 1,555,480 - Pf6N2E2_1321 0.78 -0.2 1,555,480 - Pf6N2E2_1321 0.78 -0.4 1,555,630 + +0.8 1,555,648 - -1.5 1,555,669 - +0.8 1,555,723 + +0.8
Or see this region's nucleotide sequence