Experiment: Ying_Nucleobase14 rep C; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_100 and Pf6N2E2_101 are separated by 128 nucleotides Pf6N2E2_101 and Pf6N2E2_102 are separated by 338 nucleotides
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Pf6N2E2_101 - CigR (Putative inner membrane protein), at 145,493 to 146,005
_101
Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188
_102
Position (kb) 145
146
147 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.600 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.631 kb on + strand, within Pf6N2E2_100 at 144.827 kb on - strand, within Pf6N2E2_100 at 144.900 kb on + strand, within Pf6N2E2_100 at 144.901 kb on - strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.961 kb on + strand, within Pf6N2E2_100 at 144.979 kb on + strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 145.000 kb on + strand, within Pf6N2E2_100 at 145.001 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.136 kb on - strand, within Pf6N2E2_100 at 145.352 kb on - strand at 145.352 kb on - strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.393 kb on + strand at 145.393 kb on + strand at 145.433 kb on + strand at 145.434 kb on - strand at 145.434 kb on - strand at 145.436 kb on + strand at 145.437 kb on - strand at 145.437 kb on - strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.460 kb on + strand at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.631 kb on - strand, within Pf6N2E2_101 at 145.907 kb on + strand, within Pf6N2E2_101 at 145.908 kb on - strand, within Pf6N2E2_101 at 145.917 kb on - strand, within Pf6N2E2_101 at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 146.004 kb on - strand at 146.014 kb on - strand at 146.027 kb on + strand at 146.027 kb on + strand at 146.028 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.342 kb on + strand at 146.372 kb on + strand at 146.372 kb on + strand at 146.372 kb on + strand at 146.372 kb on + strand at 146.373 kb on - strand at 146.373 kb on - strand at 146.373 kb on - strand at 146.647 kb on + strand, within Pf6N2E2_102 at 146.647 kb on + strand, within Pf6N2E2_102 at 146.647 kb on + strand, within Pf6N2E2_102 at 146.648 kb on - strand, within Pf6N2E2_102 at 146.774 kb on + strand, within Pf6N2E2_102 at 146.775 kb on - strand, within Pf6N2E2_102 at 146.777 kb on + strand, within Pf6N2E2_102 at 146.777 kb on + strand, within Pf6N2E2_102 at 146.778 kb on - strand, within Pf6N2E2_102 at 146.778 kb on - strand, within Pf6N2E2_102 at 146.887 kb on + strand, within Pf6N2E2_102
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep C; time point 1 remove 144,511 + Pf6N2E2_100 0.30 +1.0 144,511 + Pf6N2E2_100 0.30 +1.0 144,512 - Pf6N2E2_100 0.30 +1.0 144,512 - Pf6N2E2_100 0.30 +1.0 144,600 + Pf6N2E2_100 0.37 -1.5 144,610 + Pf6N2E2_100 0.38 -0.0 144,610 + Pf6N2E2_100 0.38 +1.4 144,610 + Pf6N2E2_100 0.38 +1.0 144,631 + Pf6N2E2_100 0.39 +1.0 144,827 - Pf6N2E2_100 0.56 -0.6 144,900 + Pf6N2E2_100 0.62 +1.0 144,901 - Pf6N2E2_100 0.62 +1.0 144,951 + Pf6N2E2_100 0.66 -1.4 144,951 + Pf6N2E2_100 0.66 +1.0 144,961 + Pf6N2E2_100 0.67 +0.3 144,979 + Pf6N2E2_100 0.68 +1.0 144,980 - Pf6N2E2_100 0.68 -0.6 144,980 - Pf6N2E2_100 0.68 -3.2 144,980 - Pf6N2E2_100 0.68 -0.0 144,985 + Pf6N2E2_100 0.69 +1.0 144,985 + Pf6N2E2_100 0.69 +1.0 144,986 - Pf6N2E2_100 0.69 -0.0 144,986 - Pf6N2E2_100 0.69 -0.6 144,986 - Pf6N2E2_100 0.69 -0.0 144,986 - Pf6N2E2_100 0.69 -0.4 144,986 - Pf6N2E2_100 0.69 -0.0 145,000 + Pf6N2E2_100 0.70 -0.1 145,001 - Pf6N2E2_100 0.70 +1.0 145,079 - Pf6N2E2_100 0.76 -0.0 145,079 - Pf6N2E2_100 0.76 -0.5 145,136 - Pf6N2E2_100 0.81 -0.6 145,352 - +1.0 145,352 - -0.0 145,391 + -0.5 145,391 + +1.0 145,391 + +1.0 145,391 + +1.0 145,391 + +1.0 145,392 - -1.4 145,392 - +1.0 145,392 - -0.0 145,392 - +1.0 145,393 + +1.0 145,393 + -0.4 145,433 + -2.1 145,434 - +1.0 145,434 - +1.0 145,436 + -1.0 145,437 - +1.0 145,437 - -0.0 145,452 + +1.0 145,452 + +1.0 145,452 + -1.5 145,452 + +1.0 145,452 + -1.5 145,452 + -1.0 145,453 - +1.0 145,453 - +1.5 145,453 - +1.0 145,453 - -0.6 145,454 + +1.0 145,454 + -0.0 145,454 + +1.0 145,454 + +1.0 145,455 - +1.0 145,455 - +1.0 145,455 - -0.0 145,460 + +1.0 145,630 + Pf6N2E2_101 0.27 +1.0 145,630 + Pf6N2E2_101 0.27 -0.0 145,630 + Pf6N2E2_101 0.27 -1.0 145,631 - Pf6N2E2_101 0.27 -0.6 145,907 + Pf6N2E2_101 0.81 -1.5 145,908 - Pf6N2E2_101 0.81 +1.0 145,917 - Pf6N2E2_101 0.83 -0.0 145,976 + +1.0 145,976 + +1.0 145,976 + +1.0 145,976 + +1.0 145,976 + -0.0 145,977 - -1.0 145,977 - -1.4 145,977 - -0.4 145,977 - +1.0 145,977 - +0.1 145,977 - +1.0 146,004 - +1.5 146,014 - +1.0 146,027 + +1.0 146,027 + -0.8 146,028 - -0.4 146,143 - -0.5 146,143 - +1.0 146,143 - -0.0 146,268 + +1.0 146,268 + +1.0 146,268 + -0.0 146,294 + -2.4 146,294 + -0.6 146,294 + +1.0 146,298 + +0.2 146,298 + -0.6 146,298 + -0.6 146,298 + -0.0 146,298 + -0.0 146,298 + -2.9 146,342 + +1.0 146,372 + -0.6 146,372 + -1.4 146,372 + -1.0 146,372 + +1.0 146,373 - +1.0 146,373 - +1.0 146,373 - -0.6 146,647 + Pf6N2E2_102 0.16 -1.0 146,647 + Pf6N2E2_102 0.16 +1.0 146,647 + Pf6N2E2_102 0.16 -0.0 146,648 - Pf6N2E2_102 0.16 -0.0 146,774 + Pf6N2E2_102 0.23 -0.0 146,775 - Pf6N2E2_102 0.23 +1.0 146,777 + Pf6N2E2_102 0.23 -1.1 146,777 + Pf6N2E2_102 0.23 -0.0 146,778 - Pf6N2E2_102 0.24 -0.0 146,778 - Pf6N2E2_102 0.24 +1.0 146,887 + Pf6N2E2_102 0.29 -0.0
Or see this region's nucleotide sequence