Experiment: Ying_Nucleobase14 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5918 and Pf6N2E2_5919 are separated by 22 nucleotides Pf6N2E2_5919 and Pf6N2E2_5920 overlap by 4 nucleotides Pf6N2E2_5920 and Pf6N2E2_5921 are separated by 478 nucleotides
Pf6N2E2_5918 - hypothetical protein, at 6,737,805 to 6,738,074
_5918
Pf6N2E2_5919 - Flp pilus assembly protein TadD, contains TPR repeats, at 6,738,097 to 6,738,870
_5919
Pf6N2E2_5920 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 6,738,867 to 6,740,090
_5920
Pf6N2E2_5921 - Metal-dependent hydrolase related to alanyl-tRNA synthetase, at 6,740,569 to 6,741,195
_5921
Position (kb)
6738
6739
6740
6741 Strain fitness (log2 ratio)
-2
-1
0
1 at 6738.091 kb on - strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.098 kb on + strand at 6738.099 kb on - strand at 6738.099 kb on - strand at 6738.276 kb on - strand, within Pf6N2E2_5919 at 6738.384 kb on - strand, within Pf6N2E2_5919 at 6738.449 kb on + strand, within Pf6N2E2_5919 at 6738.653 kb on + strand, within Pf6N2E2_5919 at 6738.654 kb on - strand, within Pf6N2E2_5919 at 6738.815 kb on + strand at 6738.816 kb on - strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.916 kb on + strand at 6738.917 kb on - strand at 6738.917 kb on - strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.920 kb on + strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.921 kb on - strand at 6738.940 kb on + strand at 6738.941 kb on - strand at 6739.343 kb on - strand, within Pf6N2E2_5920 at 6739.457 kb on + strand, within Pf6N2E2_5920 at 6739.459 kb on + strand, within Pf6N2E2_5920 at 6739.505 kb on + strand, within Pf6N2E2_5920 at 6739.506 kb on - strand, within Pf6N2E2_5920 at 6739.722 kb on + strand, within Pf6N2E2_5920 at 6739.723 kb on - strand, within Pf6N2E2_5920 at 6739.789 kb on - strand, within Pf6N2E2_5920 at 6740.064 kb on + strand at 6740.064 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.078 kb on + strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.079 kb on - strand at 6740.120 kb on + strand at 6740.121 kb on - strand at 6740.130 kb on + strand at 6740.130 kb on + strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.131 kb on - strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.148 kb on + strand at 6740.149 kb on - strand at 6740.149 kb on - strand at 6740.381 kb on + strand at 6740.382 kb on - strand at 6740.426 kb on + strand at 6740.582 kb on + strand at 6740.583 kb on - strand at 6740.583 kb on - strand at 6740.596 kb on + strand at 6740.596 kb on + strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.597 kb on - strand at 6740.679 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.825 kb on - strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.990 kb on + strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6740.991 kb on - strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921 at 6741.025 kb on + strand, within Pf6N2E2_5921
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1 remove 6,738,091 - -2.0 6,738,098 + -0.5 6,738,098 + -0.4 6,738,098 + -1.0 6,738,098 + +1.0 6,738,098 + +1.0 6,738,099 - +1.0 6,738,099 - +1.0 6,738,276 - Pf6N2E2_5919 0.23 -1.3 6,738,384 - Pf6N2E2_5919 0.37 -0.2 6,738,449 + Pf6N2E2_5919 0.45 +1.0 6,738,653 + Pf6N2E2_5919 0.72 +0.8 6,738,654 - Pf6N2E2_5919 0.72 +1.0 6,738,815 + +1.0 6,738,816 - -0.8 6,738,916 + -1.6 6,738,916 + -0.5 6,738,916 + +0.2 6,738,916 + -0.5 6,738,916 + -0.3 6,738,917 - +1.0 6,738,917 - +0.0 6,738,920 + -0.2 6,738,920 + -0.8 6,738,920 + -1.0 6,738,920 + +0.0 6,738,920 + +0.0 6,738,920 + -1.0 6,738,920 + -1.8 6,738,920 + -0.4 6,738,920 + +1.0 6,738,921 - +0.0 6,738,921 - +1.0 6,738,921 - +1.0 6,738,921 - +1.0 6,738,921 - -0.6 6,738,921 - +1.0 6,738,921 - +0.0 6,738,921 - +1.0 6,738,921 - -1.0 6,738,921 - +0.5 6,738,940 + -1.6 6,738,941 - +1.0 6,739,343 - Pf6N2E2_5920 0.39 +0.0 6,739,457 + Pf6N2E2_5920 0.48 -1.0 6,739,459 + Pf6N2E2_5920 0.48 +1.0 6,739,505 + Pf6N2E2_5920 0.52 -1.0 6,739,506 - Pf6N2E2_5920 0.52 +1.0 6,739,722 + Pf6N2E2_5920 0.70 -1.2 6,739,723 - Pf6N2E2_5920 0.70 -0.6 6,739,789 - Pf6N2E2_5920 0.75 -1.0 6,740,064 + +1.0 6,740,064 + +1.0 6,740,078 + +1.0 6,740,078 + +0.0 6,740,078 + +0.0 6,740,079 - -1.4 6,740,079 - +1.0 6,740,079 - -0.6 6,740,120 + -0.7 6,740,121 - +1.0 6,740,130 + -0.3 6,740,130 + +0.4 6,740,131 - +1.0 6,740,131 - +0.0 6,740,131 - +0.0 6,740,148 + +1.0 6,740,148 + -1.0 6,740,148 + +1.0 6,740,148 + +0.0 6,740,148 + +1.0 6,740,149 - -0.1 6,740,149 - -1.3 6,740,381 + +1.0 6,740,382 - +0.0 6,740,426 + -0.7 6,740,582 + +1.0 6,740,583 - -0.4 6,740,583 - -0.5 6,740,596 + -0.3 6,740,596 + +0.0 6,740,597 - -0.6 6,740,597 - +0.0 6,740,597 - -0.2 6,740,679 - Pf6N2E2_5921 0.18 -1.0 6,740,825 - Pf6N2E2_5921 0.41 -1.2 6,740,825 - Pf6N2E2_5921 0.41 +0.0 6,740,990 + Pf6N2E2_5921 0.67 +1.0 6,740,990 + Pf6N2E2_5921 0.67 -0.6 6,740,990 + Pf6N2E2_5921 0.67 +1.0 6,740,991 - Pf6N2E2_5921 0.67 +1.0 6,740,991 - Pf6N2E2_5921 0.67 -1.3 6,741,025 + Pf6N2E2_5921 0.73 +0.0 6,741,025 + Pf6N2E2_5921 0.73 +1.0 6,741,025 + Pf6N2E2_5921 0.73 -0.6
Or see this region's nucleotide sequence