Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_474

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_473 and Pf6N2E2_474 are separated by 82 nucleotidesPf6N2E2_474 and Pf6N2E2_475 are separated by 76 nucleotidesPf6N2E2_475 and Pf6N2E2_476 are separated by 110 nucleotides Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272 _473 Pf6N2E2_474 - Transcriptional regulator, ArsR family, at 539,355 to 540,110 _474 Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981 _475 Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021 _476 Position (kb) 539 540 541Strain fitness (log2 ratio) -2 -1 0 1at 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.617 kb on + strand, within Pf6N2E2_473at 538.618 kb on - strand, within Pf6N2E2_473at 538.655 kb on - strand, within Pf6N2E2_473at 538.655 kb on - strand, within Pf6N2E2_473at 538.736 kb on + strand, within Pf6N2E2_473at 539.227 kb on - strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.309 kb on + strandat 539.310 kb on - strandat 539.310 kb on - strandat 539.312 kb on + strandat 539.577 kb on + strand, within Pf6N2E2_474at 539.577 kb on + strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.578 kb on - strand, within Pf6N2E2_474at 539.697 kb on + strand, within Pf6N2E2_474at 539.705 kb on + strand, within Pf6N2E2_474at 539.706 kb on - strand, within Pf6N2E2_474at 540.198 kb on + strandat 540.198 kb on + strandat 540.710 kb on + strand, within Pf6N2E2_475at 540.711 kb on - strand, within Pf6N2E2_475at 540.711 kb on - strand, within Pf6N2E2_475at 540.800 kb on + strand, within Pf6N2E2_475at 540.801 kb on - strand, within Pf6N2E2_475at 540.801 kb on - strand, within Pf6N2E2_475at 540.839 kb on + strand, within Pf6N2E2_475at 540.839 kb on + strand, within Pf6N2E2_475at 540.839 kb on + strand, within Pf6N2E2_475at 540.840 kb on - strand, within Pf6N2E2_475at 540.840 kb on - strand, within Pf6N2E2_475at 540.840 kb on - strand, within Pf6N2E2_475at 540.840 kb on - strand, within Pf6N2E2_475at 540.840 kb on - strand, within Pf6N2E2_475at 540.845 kb on + strand, within Pf6N2E2_475at 540.882 kb on - strand, within Pf6N2E2_475at 540.981 kb on + strandat 541.028 kb on + strandat 541.028 kb on + strandat 541.029 kb on - strandat 541.029 kb on - strandat 541.029 kb on - strandat 541.029 kb on - strandat 541.051 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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538,617 + Pf6N2E2_473 0.42 +0.9
538,617 + Pf6N2E2_473 0.42 -0.7
538,617 + Pf6N2E2_473 0.42 -0.1
538,617 + Pf6N2E2_473 0.42 +0.2
538,618 - Pf6N2E2_473 0.42 -0.1
538,655 - Pf6N2E2_473 0.46 +0.9
538,655 - Pf6N2E2_473 0.46 +0.9
538,736 + Pf6N2E2_473 0.53 +0.9
539,227 - -1.0
539,309 + -0.9
539,309 + +0.9
539,309 + -2.5
539,309 + +0.9
539,309 + +0.2
539,309 + -0.5
539,309 + +0.9
539,310 - -0.1
539,310 - +0.9
539,312 + +0.9
539,577 + Pf6N2E2_474 0.29 -1.4
539,577 + Pf6N2E2_474 0.29 -2.7
539,578 - Pf6N2E2_474 0.29 +0.9
539,578 - Pf6N2E2_474 0.29 +0.9
539,578 - Pf6N2E2_474 0.29 -0.9
539,697 + Pf6N2E2_474 0.45 -1.7
539,705 + Pf6N2E2_474 0.46 +0.9
539,706 - Pf6N2E2_474 0.46 -0.1
540,198 + +0.9
540,198 + -0.1
540,710 + Pf6N2E2_475 0.66 +0.9
540,711 - Pf6N2E2_475 0.66 +0.9
540,711 - Pf6N2E2_475 0.66 +0.9
540,800 + Pf6N2E2_475 0.77 -0.1
540,801 - Pf6N2E2_475 0.77 -0.1
540,801 - Pf6N2E2_475 0.77 -0.6
540,839 + Pf6N2E2_475 0.82 -1.6
540,839 + Pf6N2E2_475 0.82 +0.9
540,839 + Pf6N2E2_475 0.82 +0.9
540,840 - Pf6N2E2_475 0.82 +0.9
540,840 - Pf6N2E2_475 0.82 -0.1
540,840 - Pf6N2E2_475 0.82 +0.9
540,840 - Pf6N2E2_475 0.82 -0.7
540,840 - Pf6N2E2_475 0.82 +0.9
540,845 + Pf6N2E2_475 0.83 -0.1
540,882 - Pf6N2E2_475 0.87 +0.9
540,981 + +0.9
541,028 + +0.9
541,028 + +0.9
541,029 - -0.6
541,029 - +0.9
541,029 - +0.9
541,029 - -0.1
541,051 - -0.7

Or see this region's nucleotide sequence