Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4518

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4517 and Pf6N2E2_4518 are separated by 235 nucleotidesPf6N2E2_4518 and Pf6N2E2_4519 are separated by 102 nucleotides Pf6N2E2_4517 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 5,250,180 to 5,251,067 _4517 Pf6N2E2_4518 - Carboxymuconolactone decarboxylase family protein, at 5,251,303 to 5,251,644 _4518 Pf6N2E2_4519 - Penicillin acylase II (EC 3.5.1.11), at 5,251,747 to 5,254,155 _4519 Position (kb) 5251 5252Strain fitness (log2 ratio) -2 -1 0 1at 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.319 kb on - strand, within Pf6N2E2_4517at 5250.385 kb on - strand, within Pf6N2E2_4517at 5250.624 kb on + strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.625 kb on - strand, within Pf6N2E2_4517at 5250.978 kb on - strand, within Pf6N2E2_4517at 5251.066 kb on - strandat 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.346 kb on + strand, within Pf6N2E2_4518at 5251.347 kb on - strand, within Pf6N2E2_4518at 5251.350 kb on + strand, within Pf6N2E2_4518at 5251.350 kb on + strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.351 kb on - strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.352 kb on + strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.353 kb on - strand, within Pf6N2E2_4518at 5251.444 kb on + strand, within Pf6N2E2_4518at 5251.445 kb on - strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.556 kb on + strand, within Pf6N2E2_4518at 5251.557 kb on - strand, within Pf6N2E2_4518at 5251.615 kb on - strandat 5251.615 kb on - strandat 5251.636 kb on - strandat 5251.636 kb on - strandat 5251.687 kb on + strandat 5251.687 kb on + strandat 5251.688 kb on - strandat 5251.688 kb on - strandat 5251.688 kb on - strandat 5251.722 kb on + strandat 5251.734 kb on - strandat 5251.771 kb on + strandat 5251.772 kb on - strandat 5251.832 kb on + strandat 5251.832 kb on + strandat 5251.832 kb on + strandat 5251.833 kb on - strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.847 kb on + strandat 5251.848 kb on - strandat 5251.848 kb on - strandat 5251.848 kb on - strandat 5251.869 kb on - strandat 5251.956 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.967 kb on + strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5251.968 kb on - strandat 5252.109 kb on + strand, within Pf6N2E2_4519at 5252.110 kb on - strand, within Pf6N2E2_4519at 5252.110 kb on - strand, within Pf6N2E2_4519at 5252.110 kb on - strand, within Pf6N2E2_4519at 5252.621 kb on + strand, within Pf6N2E2_4519at 5252.621 kb on + strand, within Pf6N2E2_4519at 5252.621 kb on + strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519at 5252.622 kb on - strand, within Pf6N2E2_4519

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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5,250,319 - Pf6N2E2_4517 0.16 +0.0
5,250,319 - Pf6N2E2_4517 0.16 +1.0
5,250,385 - Pf6N2E2_4517 0.23 +0.0
5,250,624 + Pf6N2E2_4517 0.50 +0.0
5,250,625 - Pf6N2E2_4517 0.50 +1.0
5,250,625 - Pf6N2E2_4517 0.50 -0.5
5,250,625 - Pf6N2E2_4517 0.50 -0.6
5,250,978 - Pf6N2E2_4517 0.90 -0.6
5,251,066 - +0.0
5,251,346 + Pf6N2E2_4518 0.13 +1.0
5,251,346 + Pf6N2E2_4518 0.13 +1.0
5,251,346 + Pf6N2E2_4518 0.13 -1.3
5,251,346 + Pf6N2E2_4518 0.13 +1.0
5,251,346 + Pf6N2E2_4518 0.13 +1.0
5,251,346 + Pf6N2E2_4518 0.13 +0.0
5,251,347 - Pf6N2E2_4518 0.13 -0.5
5,251,350 + Pf6N2E2_4518 0.14 +0.0
5,251,350 + Pf6N2E2_4518 0.14 -0.2
5,251,351 - Pf6N2E2_4518 0.14 +1.0
5,251,351 - Pf6N2E2_4518 0.14 -0.6
5,251,351 - Pf6N2E2_4518 0.14 -1.3
5,251,351 - Pf6N2E2_4518 0.14 +0.3
5,251,352 + Pf6N2E2_4518 0.14 +1.0
5,251,352 + Pf6N2E2_4518 0.14 -0.9
5,251,352 + Pf6N2E2_4518 0.14 -1.1
5,251,352 + Pf6N2E2_4518 0.14 +0.0
5,251,353 - Pf6N2E2_4518 0.15 -0.6
5,251,353 - Pf6N2E2_4518 0.15 -0.6
5,251,353 - Pf6N2E2_4518 0.15 +0.0
5,251,353 - Pf6N2E2_4518 0.15 -0.9
5,251,353 - Pf6N2E2_4518 0.15 +1.0
5,251,353 - Pf6N2E2_4518 0.15 +0.0
5,251,353 - Pf6N2E2_4518 0.15 -1.0
5,251,444 + Pf6N2E2_4518 0.41 +0.0
5,251,445 - Pf6N2E2_4518 0.42 +1.0
5,251,556 + Pf6N2E2_4518 0.74 -0.6
5,251,556 + Pf6N2E2_4518 0.74 -1.4
5,251,556 + Pf6N2E2_4518 0.74 -0.6
5,251,557 - Pf6N2E2_4518 0.74 +0.0
5,251,615 - +0.0
5,251,615 - +1.0
5,251,636 - -1.3
5,251,636 - -0.2
5,251,687 + +0.0
5,251,687 + -0.6
5,251,688 - -1.8
5,251,688 - +0.0
5,251,688 - +0.0
5,251,722 + -1.0
5,251,734 - +0.0
5,251,771 + -0.6
5,251,772 - -0.5
5,251,832 + +1.0
5,251,832 + +0.0
5,251,832 + -0.3
5,251,833 - +1.0
5,251,847 + -0.6
5,251,847 + -0.6
5,251,847 + +0.0
5,251,847 + -1.0
5,251,848 - +1.0
5,251,848 - +1.0
5,251,848 - +1.0
5,251,869 - -0.6
5,251,956 + -0.6
5,251,967 + +1.0
5,251,967 + +0.0
5,251,967 + +0.0
5,251,967 + +1.0
5,251,967 + +0.0
5,251,967 + -0.7
5,251,967 + +0.0
5,251,967 + +1.0
5,251,967 + +1.0
5,251,967 + -0.6
5,251,968 - -0.6
5,251,968 - +0.0
5,251,968 - -0.6
5,251,968 - -0.9
5,251,968 - +0.0
5,251,968 - -1.2
5,251,968 - -1.6
5,251,968 - +0.0
5,251,968 - +0.0
5,251,968 - -0.7
5,251,968 - -0.5
5,252,109 + Pf6N2E2_4519 0.15 -0.6
5,252,110 - Pf6N2E2_4519 0.15 +1.0
5,252,110 - Pf6N2E2_4519 0.15 +1.0
5,252,110 - Pf6N2E2_4519 0.15 +1.0
5,252,621 + Pf6N2E2_4519 0.36 +1.0
5,252,621 + Pf6N2E2_4519 0.36 -0.6
5,252,621 + Pf6N2E2_4519 0.36 -1.0
5,252,622 - Pf6N2E2_4519 0.36 +0.0
5,252,622 - Pf6N2E2_4519 0.36 -0.7
5,252,622 - Pf6N2E2_4519 0.36 -0.6
5,252,622 - Pf6N2E2_4519 0.36 +0.0
5,252,622 - Pf6N2E2_4519 0.36 +1.0
5,252,622 - Pf6N2E2_4519 0.36 -1.3
5,252,622 - Pf6N2E2_4519 0.36 -0.9

Or see this region's nucleotide sequence