Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_261

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_259 and Pf6N2E2_260 are separated by 56 nucleotidesPf6N2E2_260 and Pf6N2E2_261 are separated by 198 nucleotidesPf6N2E2_261 and Pf6N2E2_262 are separated by 70 nucleotidesPf6N2E2_262 and Pf6N2E2_263 are separated by 263 nucleotides Pf6N2E2_259 - Transcriptional regulator, LysR family, at 323,206 to 324,084 _259 Pf6N2E2_260 - Regulator of cell morphogenesis and NO signaling, at 324,141 to 324,617 _260 Pf6N2E2_261 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 324,816 to 325,925 _261 Pf6N2E2_262 - hypothetical protein, at 325,996 to 326,292 _262 Pf6N2E2_263 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 326,556 to 327,200 _263 Position (kb) 324 325 326Strain fitness (log2 ratio) -2 -1 0 1 2at 323.914 kb on + strand, within Pf6N2E2_259at 323.914 kb on + strand, within Pf6N2E2_259at 323.914 kb on + strand, within Pf6N2E2_259at 323.915 kb on - strand, within Pf6N2E2_259at 323.915 kb on - strand, within Pf6N2E2_259at 323.915 kb on - strand, within Pf6N2E2_259at 323.915 kb on - strand, within Pf6N2E2_259at 323.915 kb on - strand, within Pf6N2E2_259at 324.083 kb on - strandat 324.083 kb on - strandat 324.128 kb on + strandat 324.128 kb on + strandat 324.129 kb on - strandat 324.179 kb on - strandat 324.383 kb on + strand, within Pf6N2E2_260at 324.406 kb on + strand, within Pf6N2E2_260at 324.406 kb on + strand, within Pf6N2E2_260at 324.407 kb on - strand, within Pf6N2E2_260at 324.409 kb on + strand, within Pf6N2E2_260at 324.409 kb on + strand, within Pf6N2E2_260at 324.410 kb on - strand, within Pf6N2E2_260at 324.617 kb on + strandat 324.617 kb on + strandat 324.617 kb on + strandat 324.617 kb on + strandat 324.617 kb on + strandat 324.617 kb on + strandat 324.617 kb on + strandat 324.618 kb on - strandat 324.618 kb on - strandat 324.618 kb on - strandat 324.618 kb on - strandat 324.621 kb on + strandat 324.621 kb on + strandat 324.621 kb on + strandat 324.621 kb on + strandat 324.621 kb on + strandat 324.635 kb on + strandat 325.034 kb on - strand, within Pf6N2E2_261at 325.228 kb on + strand, within Pf6N2E2_261at 325.228 kb on + strand, within Pf6N2E2_261at 325.229 kb on - strand, within Pf6N2E2_261at 325.408 kb on - strand, within Pf6N2E2_261at 325.507 kb on + strand, within Pf6N2E2_261at 325.507 kb on + strand, within Pf6N2E2_261at 325.508 kb on - strand, within Pf6N2E2_261at 325.609 kb on - strand, within Pf6N2E2_261at 325.609 kb on - strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.696 kb on + strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.697 kb on - strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.702 kb on + strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.703 kb on - strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.759 kb on + strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.760 kb on - strand, within Pf6N2E2_261at 325.965 kb on + strandat 326.096 kb on + strand, within Pf6N2E2_262at 326.097 kb on - strand, within Pf6N2E2_262at 326.253 kb on - strand, within Pf6N2E2_262at 326.265 kb on + strandat 326.266 kb on - strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.465 kb on + strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.466 kb on - strandat 326.553 kb on + strandat 326.553 kb on + strandat 326.554 kb on - strandat 326.554 kb on - strandat 326.554 kb on - strandat 326.558 kb on - strandat 326.558 kb on - strandat 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.644 kb on + strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.645 kb on - strand, within Pf6N2E2_263at 326.737 kb on + strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.738 kb on - strand, within Pf6N2E2_263at 326.784 kb on + strand, within Pf6N2E2_263at 326.849 kb on + strand, within Pf6N2E2_263at 326.850 kb on - strand, within Pf6N2E2_263

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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323,914 + Pf6N2E2_259 0.81 -0.9
323,914 + Pf6N2E2_259 0.81 -0.1
323,914 + Pf6N2E2_259 0.81 -0.2
323,915 - Pf6N2E2_259 0.81 +0.9
323,915 - Pf6N2E2_259 0.81 +0.3
323,915 - Pf6N2E2_259 0.81 +0.9
323,915 - Pf6N2E2_259 0.81 -0.6
323,915 - Pf6N2E2_259 0.81 -1.9
324,083 - -0.3
324,083 - +0.9
324,128 + +0.9
324,128 + +0.9
324,129 - -0.1
324,179 - -0.7
324,383 + Pf6N2E2_260 0.51 +0.9
324,406 + Pf6N2E2_260 0.56 -0.1
324,406 + Pf6N2E2_260 0.56 +0.9
324,407 - Pf6N2E2_260 0.56 +0.9
324,409 + Pf6N2E2_260 0.56 +0.9
324,409 + Pf6N2E2_260 0.56 -1.4
324,410 - Pf6N2E2_260 0.56 +0.9
324,617 + +0.9
324,617 + -0.1
324,617 + +0.9
324,617 + +0.9
324,617 + -0.7
324,617 + +0.9
324,617 + -0.1
324,618 - -0.1
324,618 - -0.1
324,618 - +0.9
324,618 - -0.1
324,621 + +0.9
324,621 + +0.9
324,621 + +0.9
324,621 + +0.9
324,621 + -0.7
324,635 + -0.1
325,034 - Pf6N2E2_261 0.20 +0.9
325,228 + Pf6N2E2_261 0.37 +0.9
325,228 + Pf6N2E2_261 0.37 +0.9
325,229 - Pf6N2E2_261 0.37 -0.3
325,408 - Pf6N2E2_261 0.53 -0.1
325,507 + Pf6N2E2_261 0.62 -0.4
325,507 + Pf6N2E2_261 0.62 -0.3
325,508 - Pf6N2E2_261 0.62 +0.9
325,609 - Pf6N2E2_261 0.71 -0.7
325,609 - Pf6N2E2_261 0.71 +0.9
325,696 + Pf6N2E2_261 0.79 -1.4
325,696 + Pf6N2E2_261 0.79 +0.9
325,696 + Pf6N2E2_261 0.79 -0.1
325,697 - Pf6N2E2_261 0.79 +0.9
325,697 - Pf6N2E2_261 0.79 +0.9
325,697 - Pf6N2E2_261 0.79 -0.1
325,702 + Pf6N2E2_261 0.80 -0.7
325,702 + Pf6N2E2_261 0.80 +0.9
325,702 + Pf6N2E2_261 0.80 +0.9
325,702 + Pf6N2E2_261 0.80 -0.9
325,703 - Pf6N2E2_261 0.80 -1.1
325,703 - Pf6N2E2_261 0.80 -2.4
325,703 - Pf6N2E2_261 0.80 +0.9
325,703 - Pf6N2E2_261 0.80 +0.9
325,759 + Pf6N2E2_261 0.85 +0.9
325,759 + Pf6N2E2_261 0.85 -0.7
325,759 + Pf6N2E2_261 0.85 +0.9
325,759 + Pf6N2E2_261 0.85 -1.3
325,759 + Pf6N2E2_261 0.85 +0.9
325,759 + Pf6N2E2_261 0.85 -1.1
325,759 + Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 -0.1
325,760 - Pf6N2E2_261 0.85 -1.9
325,760 - Pf6N2E2_261 0.85 -0.1
325,760 - Pf6N2E2_261 0.85 -0.7
325,760 - Pf6N2E2_261 0.85 -0.1
325,760 - Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 -0.7
325,760 - Pf6N2E2_261 0.85 -0.7
325,760 - Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 +0.9
325,760 - Pf6N2E2_261 0.85 -0.7
325,965 + -0.7
326,096 + Pf6N2E2_262 0.34 -0.1
326,097 - Pf6N2E2_262 0.34 -0.7
326,253 - Pf6N2E2_262 0.87 +0.9
326,265 + -0.1
326,266 - +1.9
326,465 + -0.7
326,465 + +0.7
326,465 + -0.1
326,465 + -0.1
326,466 - +0.9
326,466 - -0.7
326,466 - +0.9
326,466 - -0.9
326,553 + -0.6
326,553 + -0.1
326,554 - -1.4
326,554 - +0.9
326,554 - +0.9
326,558 - +0.5
326,558 - -0.9
326,644 + Pf6N2E2_263 0.14 +0.9
326,644 + Pf6N2E2_263 0.14 -1.1
326,644 + Pf6N2E2_263 0.14 -0.7
326,644 + Pf6N2E2_263 0.14 +0.9
326,645 - Pf6N2E2_263 0.14 +0.9
326,645 - Pf6N2E2_263 0.14 +0.9
326,645 - Pf6N2E2_263 0.14 +0.9
326,645 - Pf6N2E2_263 0.14 -0.7
326,737 + Pf6N2E2_263 0.28 +0.9
326,738 - Pf6N2E2_263 0.28 +0.9
326,738 - Pf6N2E2_263 0.28 +0.9
326,738 - Pf6N2E2_263 0.28 -0.5
326,738 - Pf6N2E2_263 0.28 +0.9
326,784 + Pf6N2E2_263 0.35 +0.9
326,849 + Pf6N2E2_263 0.45 -0.1
326,850 - Pf6N2E2_263 0.46 +0.9

Or see this region's nucleotide sequence