Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_188

Experiment: Ying_Nucleobase14 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_186 and Pf6N2E2_187 are separated by 10 nucleotidesPf6N2E2_187 and Pf6N2E2_188 are separated by 132 nucleotidesPf6N2E2_188 and Pf6N2E2_189 are separated by 150 nucleotidesPf6N2E2_189 and Pf6N2E2_190 are separated by 14 nucleotides Pf6N2E2_186 - Urea ABC transporter, ATPase protein UrtD, at 239,149 to 239,907 _186 Pf6N2E2_187 - Urea ABC transporter, ATPase protein UrtE, at 239,918 to 240,607 _187 Pf6N2E2_188 - Formamidase (EC 3.5.1.49), at 240,740 to 241,969 _188 Pf6N2E2_189 - regulatory protein, putative, at 242,120 to 242,353 _189 Pf6N2E2_190 - Aliphatic amidase AmiE (EC 3.5.1.4), at 242,368 to 243,411 _190 Position (kb) 240 241 242Strain fitness (log2 ratio) -2 -1 0 1at 239.808 kb on + strand, within Pf6N2E2_186at 239.893 kb on + strandat 239.893 kb on + strandat 239.894 kb on - strandat 239.897 kb on + strandat 239.928 kb on + strandat 239.928 kb on + strandat 239.929 kb on - strandat 240.155 kb on + strand, within Pf6N2E2_187at 240.155 kb on + strand, within Pf6N2E2_187at 240.156 kb on - strand, within Pf6N2E2_187at 240.156 kb on - strand, within Pf6N2E2_187at 240.185 kb on - strand, within Pf6N2E2_187at 240.207 kb on + strand, within Pf6N2E2_187at 240.207 kb on + strand, within Pf6N2E2_187at 240.207 kb on + strand, within Pf6N2E2_187at 240.257 kb on + strand, within Pf6N2E2_187at 240.606 kb on - strandat 240.626 kb on + strandat 240.626 kb on + strandat 240.626 kb on + strandat 240.627 kb on - strandat 240.627 kb on - strandat 240.627 kb on - strandat 240.627 kb on - strandat 240.636 kb on + strandat 240.654 kb on + strandat 240.654 kb on + strandat 240.669 kb on - strandat 240.712 kb on + strandat 240.712 kb on + strandat 240.859 kb on + strandat 240.859 kb on + strandat 240.873 kb on - strand, within Pf6N2E2_188at 240.873 kb on - strand, within Pf6N2E2_188at 240.873 kb on - strand, within Pf6N2E2_188at 240.950 kb on + strand, within Pf6N2E2_188at 240.950 kb on + strand, within Pf6N2E2_188at 240.951 kb on - strand, within Pf6N2E2_188at 241.405 kb on + strand, within Pf6N2E2_188at 241.406 kb on - strand, within Pf6N2E2_188at 241.457 kb on + strand, within Pf6N2E2_188at 241.558 kb on + strand, within Pf6N2E2_188at 241.644 kb on - strand, within Pf6N2E2_188at 241.652 kb on + strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.653 kb on - strand, within Pf6N2E2_188at 241.669 kb on + strand, within Pf6N2E2_188at 241.752 kb on - strand, within Pf6N2E2_188at 241.752 kb on - strand, within Pf6N2E2_188at 241.788 kb on - strand, within Pf6N2E2_188at 241.822 kb on + strand, within Pf6N2E2_188at 242.015 kb on + strandat 242.015 kb on + strandat 242.015 kb on + strandat 242.016 kb on - strandat 242.021 kb on + strandat 242.022 kb on - strandat 242.222 kb on + strand, within Pf6N2E2_189at 242.222 kb on + strand, within Pf6N2E2_189at 242.318 kb on - strand, within Pf6N2E2_189at 242.318 kb on - strand, within Pf6N2E2_189at 242.351 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.360 kb on + strandat 242.383 kb on - strandat 242.425 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.445 kb on + strandat 242.446 kb on - strandat 242.545 kb on + strand, within Pf6N2E2_190at 242.566 kb on + strand, within Pf6N2E2_190at 242.566 kb on + strand, within Pf6N2E2_190at 242.567 kb on - strand, within Pf6N2E2_190at 242.567 kb on - strand, within Pf6N2E2_190at 242.601 kb on + strand, within Pf6N2E2_190at 242.713 kb on + strand, within Pf6N2E2_190at 242.714 kb on - strand, within Pf6N2E2_190at 242.789 kb on + strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.790 kb on - strand, within Pf6N2E2_190at 242.818 kb on + strand, within Pf6N2E2_190at 242.819 kb on - strand, within Pf6N2E2_190at 242.819 kb on - strand, within Pf6N2E2_190at 242.879 kb on - strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.941 kb on + strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.942 kb on - strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.943 kb on + strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190at 242.944 kb on - strand, within Pf6N2E2_190

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 1
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239,808 + Pf6N2E2_186 0.87 +0.3
239,893 + +0.9
239,893 + +0.2
239,894 - +0.9
239,897 + +0.9
239,928 + +0.9
239,928 + -0.4
239,929 - -0.1
240,155 + Pf6N2E2_187 0.34 -1.2
240,155 + Pf6N2E2_187 0.34 -0.1
240,156 - Pf6N2E2_187 0.34 +0.9
240,156 - Pf6N2E2_187 0.34 +0.9
240,185 - Pf6N2E2_187 0.39 +0.9
240,207 + Pf6N2E2_187 0.42 -1.1
240,207 + Pf6N2E2_187 0.42 -0.1
240,207 + Pf6N2E2_187 0.42 +0.9
240,257 + Pf6N2E2_187 0.49 -0.5
240,606 - -1.2
240,626 + +1.7
240,626 + +0.9
240,626 + -0.7
240,627 - +0.3
240,627 - +0.0
240,627 - -0.1
240,627 - +0.5
240,636 + -0.5
240,654 + +0.9
240,654 + +0.9
240,669 - +0.9
240,712 + +0.9
240,712 + -0.1
240,859 + +0.9
240,859 + -0.9
240,873 - Pf6N2E2_188 0.11 -0.9
240,873 - Pf6N2E2_188 0.11 -0.5
240,873 - Pf6N2E2_188 0.11 -0.7
240,950 + Pf6N2E2_188 0.17 -0.4
240,950 + Pf6N2E2_188 0.17 -0.1
240,951 - Pf6N2E2_188 0.17 +0.9
241,405 + Pf6N2E2_188 0.54 +0.9
241,406 - Pf6N2E2_188 0.54 -0.8
241,457 + Pf6N2E2_188 0.58 +0.9
241,558 + Pf6N2E2_188 0.67 +0.9
241,644 - Pf6N2E2_188 0.73 -0.1
241,652 + Pf6N2E2_188 0.74 -0.1
241,653 - Pf6N2E2_188 0.74 -0.1
241,653 - Pf6N2E2_188 0.74 +0.9
241,653 - Pf6N2E2_188 0.74 +0.9
241,669 + Pf6N2E2_188 0.76 +1.2
241,752 - Pf6N2E2_188 0.82 +0.9
241,752 - Pf6N2E2_188 0.82 +0.9
241,788 - Pf6N2E2_188 0.85 +0.9
241,822 + Pf6N2E2_188 0.88 -0.1
242,015 + +0.9
242,015 + +0.9
242,015 + -1.4
242,016 - +0.9
242,021 + -0.5
242,022 - +0.9
242,222 + Pf6N2E2_189 0.44 -0.7
242,222 + Pf6N2E2_189 0.44 -0.7
242,318 - Pf6N2E2_189 0.85 -0.6
242,318 - Pf6N2E2_189 0.85 +0.9
242,351 + +0.9
242,360 + -0.9
242,360 + -0.8
242,360 + +0.9
242,360 + -0.7
242,383 - +0.1
242,425 + +0.9
242,445 + -0.3
242,445 + +0.9
242,445 + +0.9
242,445 + -1.1
242,446 - +0.9
242,545 + Pf6N2E2_190 0.17 -0.1
242,566 + Pf6N2E2_190 0.19 -0.1
242,566 + Pf6N2E2_190 0.19 +0.9
242,567 - Pf6N2E2_190 0.19 +0.9
242,567 - Pf6N2E2_190 0.19 +0.9
242,601 + Pf6N2E2_190 0.22 -0.8
242,713 + Pf6N2E2_190 0.33 -0.1
242,714 - Pf6N2E2_190 0.33 +0.9
242,789 + Pf6N2E2_190 0.40 -0.1
242,790 - Pf6N2E2_190 0.40 -2.2
242,790 - Pf6N2E2_190 0.40 +0.9
242,790 - Pf6N2E2_190 0.40 -0.1
242,818 + Pf6N2E2_190 0.43 +0.9
242,819 - Pf6N2E2_190 0.43 -2.1
242,819 - Pf6N2E2_190 0.43 -1.0
242,879 - Pf6N2E2_190 0.49 +0.9
242,941 + Pf6N2E2_190 0.55 -0.7
242,941 + Pf6N2E2_190 0.55 -1.2
242,941 + Pf6N2E2_190 0.55 -0.2
242,941 + Pf6N2E2_190 0.55 -1.1
242,941 + Pf6N2E2_190 0.55 +0.9
242,942 - Pf6N2E2_190 0.55 +0.9
242,942 - Pf6N2E2_190 0.55 +0.9
242,942 - Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 -0.1
242,943 + Pf6N2E2_190 0.55 -0.6
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 -0.1
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 -0.7
242,943 + Pf6N2E2_190 0.55 -0.1
242,943 + Pf6N2E2_190 0.55 -0.1
242,943 + Pf6N2E2_190 0.55 -0.1
242,943 + Pf6N2E2_190 0.55 +0.9
242,943 + Pf6N2E2_190 0.55 -0.1
242,944 - Pf6N2E2_190 0.55 -0.1
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 -0.7
242,944 - Pf6N2E2_190 0.55 -1.1
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 -0.8
242,944 - Pf6N2E2_190 0.55 -0.9
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 +0.9
242,944 - Pf6N2E2_190 0.55 -0.1
242,944 - Pf6N2E2_190 0.55 +0.9

Or see this region's nucleotide sequence